A novel and sustainable composite of L@PSAC for superior removal of pharmaceuticals from different water matrices: Production, characterization, and application
Environmental Research,
Год журнала:
2024,
Номер
251, С. 118565 - 118565
Опубликована: Март 1, 2024
This
study
endeavors
to
develop
cost-effective
environmentally
friendly
technology
for
removing
harmful
residual
pharmaceuticals
from
water
and
wastewater
by
utilizing
the
effective
adsorption
of
pistachio
shell
(PS)
biochar
degradation
potency
laccase
immobilized
on
(L@PSAC).
The
carbonatization
activation
shells
were
optimized
regarding
temperature,
time,
NH4NO3/PS
ratio.
step
yielded
an
optimum
PS
(PSAC)
with
highest
porosity
surface
area
treated
at
700
°C
3
h
using
ratio
3%
wt.
immobilization
onto
PSAC
(L@PSAC)
was
its
best
level
pH
5,
60
U/g,
30
°C.
L@PSAC
maintained
a
high
enzyme
activity
over
two
months.
Almost
complete
removal
(>99%)
diclofenac,
carbamazepine,
ciprofloxacin
in
Milli-Q
(MQ)
achieved.
Adsorption
responsible
>80%
rest
facilitated
degradation.
≥60%
up
six
treatment
cycles
underscoring
promising
application
this
material
treatment.
These
results
indicate
that
activated
carbon
derived
could
potentially
be
utilized
as
carrier
adsorbent
efficiently
remove
pharmaceutical
compounds.
enzymatic
physical
elimination
approach
has
potential
used
large-scale.
Язык: Английский
Effects of modified bentonites immobilized crude enzymes on soil properties and microbial community of BaP contaminated soil
Journal of environmental chemical engineering,
Год журнала:
2025,
Номер
unknown, С. 116574 - 116574
Опубликована: Апрель 1, 2025
Язык: Английский
Immobilization of fungal laccase on peanut shell carriers
Biocatalysis and Agricultural Biotechnology,
Год журнала:
2025,
Номер
unknown, С. 103581 - 103581
Опубликована: Апрель 1, 2025
Язык: Английский
Development of a robust enzyme cascade system: co-immobilization of laccase and versatile peroxidase on polyacrylamide hydrogel for enhanced BPA degradation
Biodegradation,
Год журнала:
2025,
Номер
36(3)
Опубликована: Апрель 21, 2025
Язык: Английский
The Role of Mycoremediation in the Circular Economy
Опубликована: Янв. 1, 2025
Язык: Английский
Enhancing Laccase Production by Trametes hirsuta GMA-01 Using Response Surface Methodology and Orange Waste: A Novel Breakthrough in Sugarcane Bagasse Saccharification and Synthetic Dye Decolorization
Reactions,
Год журнала:
2024,
Номер
5(3), С. 635 - 650
Опубликована: Сен. 19, 2024
Trametes
hirsuta
GMA-01
was
cultivated
in
a
culture
medium
supplemented
with
orange
waste,
starch,
wheat
bran,
yeast
extract,
and
salts.
The
fungus
produced
several
holoenzymes,
but
the
laccase
levels
were
surprisingly
high.
Given
highlighted
applicability
of
laccases
various
biotechnological
areas
minimal
environmental
impact,
we
provided
strategy
to
increase
its
production
using
response
surface
methodology.
immobilization
into
ionic
supports
(CM-cellulose,
DEAE-agarose,
DEAE-cellulose,
DEAE-Sephacel,
MANAE-agarose,
MANAE-cellulose,
PEI-agarose)
found
be
efficient
recuperative,
showcasing
technical
prowess
research.
crude
extract
(CE)
CM-cellulose-immobilized
(ICE)
showed
optimum
activity
acidic
conditions
(pH
3.0)
at
70
°C
for
CE
60
ICE.
ICE
significantly
increased
thermostability
which
retained
21.6%
residual
after
240
min.
successfully
applied
sugarcane
bagasse
hydrolysis,
showing
13.83
±
0.02
µmol
mL−1
reducing
sugars
48
h.
Furthermore,
tested
dye
decolorization,
achieving
96.6%,
71.9%,
70.8%
decolorization
bromocresol
green,
bromophenol
blue,
orcein,
respectively
(0.05%
(w/v)
concentration).
properties
versatility
T.
different
purposes
are
interesting
notable,
opening
potential
applications
providing
valuable
insights
future
development.
Язык: Английский
Polysaccharides and Composite Adsorbents in the Spotlight for Effective Agrochemical Residue Removal from Water
Macromol—A Journal of Macromolecular Research,
Год журнала:
2024,
Номер
4(4), С. 785 - 804
Опубликована: Ноя. 6, 2024
Agrochemical
residues,
including
pesticides
and
herbicides,
pose
significant
environmental
health
risks
when
present
in
water
sources.
Conventional
treatment
methods
often
fall
short
effectively
removing
these
persistent
pollutants,
necessitating
innovative
solutions.
This
review
explores
the
use
of
polysaccharides
composite
adsorbents
as
sustainable
alternatives
for
agrochemical
residue
removal
from
water.
Biopolymers
such
chitosan,
alginate,
cellulose
are
highlighted
their
biodegradability,
biocompatibility,
ability
to
be
functionalized
enhanced
adsorption
performance.
Recent
advances
development
materials
incorporating
nanomaterials,
graphene,
oxide,
metal
oxides,
have
shown
promise
enhancing
efficiency
selectivity
adsorption.
The
also
addresses
fundamental
mechanism
adsorption,
electrostatic
interactions,
hydrogen
bonding,
hydrophobic
forces,
that
contribute
effectiveness
materials.
Challenges
associated
with
scalability,
regeneration,
real-world
applications
discussed,
well
future
opportunities
integrating
emerging
technologies
like
3D
printing
machine
learning
into
adsorbent
design.
Overall,
composites
offer
a
promising
pathway
toward
achieving
efficient
removal,
ongoing
research
needed
overcome
current
limitations
optimize
practical
application
treatment.
Язык: Английский
Polymerization potential of a bacterial CotA-laccase for β-naphthol: enzyme structure and comprehensive polymer characterization
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 21, 2024
Introduction
Laccases
are
blue-multicopper
containing
enzymes
that
known
to
play
a
role
in
the
bioconversion
of
recalcitrant
compounds.
Their
free
radical
polymerization
aromatic
compounds
for
their
valorization
remains
underexplored.
In
this
study,
we
used
pBAD
plasmid
previously
characterized
CotA
laccase
gene
(abbreviated
as
Bli
-Lacc)
from
Bacillus
licheniformis
strain
ATCC
9945a
express
enzyme
and
explore
its
biotransformation/polymerization
potential
on
β-naphthol.
Methods
The
protein
was
expressed
TOP10
cells
Escherichia
coli
after
successful
transformation
plasmid.
Immobilized
metal
affinity
chromatography
(IMAC)
generate
pure
protein.
biocatalytic
reaction
optimized
based
temperature,
pH
starting
concentration.
1
H
13
C
solution
nuclear
magnetic
resonance
(NMR),
Fourier
transform
infrared
spectroscopy
(FTIR),
solid-state
NMR
(ssNMR)
were
characterize
formed
polymer.
A
one-gram
conversion
done
applicability
pilot-scale.
Results
generated
brown
precipitate,
chemical
structure
confirmed
using
FTIR.
SsNMR
revealed
presence
two
different
orientational
hydroxyl
functional
groups
polymer
addition
very
small
amount
ether
linkages
(<
2%).
This
analysis
elucidated
occurred
mainly
carbons
rings,
rather
than
attached
groups,
resulting
condensed
ring
or
polynuclear
structure.
optimized,
highest
yield
attained
under
conditions
37°C,
10
concentration
440
nM
50
mM
phosphate
buffer.
yielded
216
mg
dry
mass.
crystal
solved
at
2.7
Å
resolution
X-ray
crystallography
presented
with
hexagonal
space
group.
final
deposited
Protein
Databank
(PDB)
an
ID−9BD5.
Discussion
article
provides
green/enzymatic
pathway
remediation
phenolics
into
useful
polymeric
materials.
comprehensive
insight
moieties
present.
Based
conversion,
synthetic
method
proves
pilot-scale
production
level
opens
opportunities
invest
industrial/environmental
applications.
Язык: Английский
Enhanced Thermostability of Laccase from Myceliophthora thermophila Through Conjugation with mPEG-SC
Catalysts,
Год журнала:
2024,
Номер
14(12), С. 887 - 887
Опубликована: Дек. 3, 2024
The
search
for
more
sustainable
reaction
conditions
has
been
necessary
to
obtain
selective
processes.
In
this
context,
laccases
have
gained
great
notoriety
in
recent
years.
However,
these
enzymes
are
unstable
organic
solvents
and
low
thermal
stability.
Alternatively,
conjugation
with
PEG
(PEGylation)
can
be
essential
overcome
problems.
work,
the
commercial
laccase
from
Myceliophthora
thermophila
(LacMT)
was
subjected
PEGylation
functionalized
as
succinimidyl
carbonate
(mPEG-SC),
followed
by
assessing
its
stability
catalytic
activity.
Mono-PEGylated
LacMT
derivatives
were
obtained,
less
than
50%
of
enzyme
remaining
native
form.
addition,
10%
bi-PEGylated
species
successfully
obtained
according
gel
electrophoresis
analysis.
PEGylated
showed
a
significantly
reduced
ABTS
oxidation
activity
(98
±
3
U/mg)
compared
(407
9
but
higher
control
without
(51
2
U/mg),
demonstrating
that
addition
activated
protein
resulted
better
protection
against
harmful
action
pH
change
required
process.
retained
twice
initial
at
40
°C
during
24
h.
exhibited
kinetic
changes,
whereas
turnover
rate
(kcat)
27%
control.
These
findings
being
reported
first
time.
This
sets
precedents
constructing
efficient
systems
involving
since
no
immobilized
biocatalyst
or
conjugate
contains
proteins.
Язык: Английский