Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

d-Allose has great potential for application in the food and pharmaceutical industries owing to its remarkable physiological properties. Most studies on d-allose production have primarily focused enzyme catalysis using Izumoring strategy, which typically requires use of expensive d-allulose as a substrate. Herein, metabolically engineered strain Escherichia coli was developed synthesize directly from inexpensive d-glucose. The synthesis pathway systematically optimized through modular metabolic engineering. functionality isomerases involved conversion confirmed vivo, while byproduct transporter pathways were blocked positively pull reversible epimerization. Gene knockouts employed weaken glycolytic pathways, redirecting carbon flux toward product synthesis. Additionally, nonphosphorylated transport d-glucose introduced enhance substrate utilization. In fed-batch fermentation, achieved titer 4.17 g/L, with yield 0.103 g/g Our achievements are expected advance industrial d-allose, this strategy is also applicable producing other rare sugars.

Язык: Английский