Enhanced Synthesis of Rare d-Allose from d-Glucose by Positively Pulling and Forcing Reversible Epimerization in Engineered Escherichia coli DOI
Qiang Guo, Mengjun Zhang,

Lingjie Zheng

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

d-Allose has great potential for application in the food and pharmaceutical industries owing to its remarkable physiological properties. Most studies on d-allose production have primarily focused enzyme catalysis using Izumoring strategy, which typically requires use of expensive d-allulose as a substrate. Herein, metabolically engineered strain Escherichia coli was developed synthesize directly from inexpensive d-glucose. The synthesis pathway systematically optimized through modular metabolic engineering. functionality isomerases involved conversion confirmed vivo, while byproduct transporter pathways were blocked positively pull reversible epimerization. Gene knockouts employed weaken glycolytic pathways, redirecting carbon flux toward product synthesis. Additionally, nonphosphorylated transport d-glucose introduced enhance substrate utilization. In fed-batch fermentation, achieved titer 4.17 g/L, with yield 0.103 g/g Our achievements are expected advance industrial d-allose, this strategy is also applicable producing other rare sugars.

Language: Английский

Advances in the bioproduction of d-allulose: A comprehensive review of current status and future prospects DOI
Yang Zhao, Xuguo Duan, Jinbo Zhang

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 202, P. 115767 - 115767

Published: Jan. 18, 2025

Language: Английский

Citations

1
Enhanced Synthesis of Rare d-Allose from d-Glucose by Positively Pulling and Forcing Reversible Epimerization in Engineered Escherichia coli DOI
Qiang Guo, Mengjun Zhang,

Lingjie Zheng

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

d-Allose has great potential for application in the food and pharmaceutical industries owing to its remarkable physiological properties. Most studies on d-allose production have primarily focused enzyme catalysis using Izumoring strategy, which typically requires use of expensive d-allulose as a substrate. Herein, metabolically engineered strain Escherichia coli was developed synthesize directly from inexpensive d-glucose. The synthesis pathway systematically optimized through modular metabolic engineering. functionality isomerases involved conversion confirmed vivo, while byproduct transporter pathways were blocked positively pull reversible epimerization. Gene knockouts employed weaken glycolytic pathways, redirecting carbon flux toward product synthesis. Additionally, nonphosphorylated transport d-glucose introduced enhance substrate utilization. In fed-batch fermentation, achieved titer 4.17 g/L, with yield 0.103 g/g Our achievements are expected advance industrial d-allose, this strategy is also applicable producing other rare sugars.

Language: Английский

Citations

0