Development of a high-speed and ultrasensitive UV/Vis-CM for detecting total triterpenes in traditional Chinese medicine and its application
Heliyon,
Год журнала:
2024,
Номер
10(11), С. e32239 - e32239
Опубликована: Май 31, 2024
This
study
proposes
a
novel
colorimetric
method
based
on
the
ultraviolet/visible
spectrophotometry-colorimetric
(UV/Vis-CM)
for
detecting
and
quantifying
total
triterpenoids
in
traditional
Chinese
medicine.
By
incorporating
colourants
2-hydroxy-5-methylbenzaldehyde
concentrated
sulfuric
acid,
triterpenoid
compounds
colour
development
became
more
sensitive,
detection
accuracy
was
significantly
improved.
acid
were
incorporated
1:3
volume
ratio
at
room
temperature
to
react
with
triterpenes
25
min,
an
ice
bath
5
then
detected
optimal
absorption
wavelength.
The
reliability
of
this
verified
by
comparison
high-performance
liquid
chromatography
four
other
methods.
Additionally,
approach
has
advantages
not
requiring
heating
during
operation,
high
sensitivity,
short
usage
time,
low
solvent
usage,
equipment
costs.
only
offers
reliable
medicine
but
also
rapid
tool
on-site
testing
large-scale
screening,
laying
foundation
modernisation
research,
quality
control,
drug
development.
Язык: Английский
Cadmium and Copper Stress Responses in Soapbark Tree (Quillaja saponaria): Effects on Growth, Metal Accumulation, Saponin Concentration, and Gene Expression
Plants,
Год журнала:
2025,
Номер
14(5), С. 709 - 709
Опубликована: Фев. 26, 2025
Heavy
metals
such
as
Cu
and
Cd
are
important
pollutants.
Quillay
(Quillaja
saponaria)
is
a
tree
species
endemic
to
Chile
that
of
worldwide
commercial
interest
due
its
saponins.
It
can
grow
on
contaminated
sites.
However,
the
biological
mechanisms
underlying
defensive
responses
remain
elusive.
This
study
aimed
characterize
plants
under
stress
identify
controlling
their
interaction
with
these
metals.
We
subjected
six-month-old
(75,
150,
300
μM)
(20,
40,
80
in
hydroponics
for
week
assessed
growth,
metal
accumulation,
saponin
production,
expression
suite
stress-induced
genes.
Those
genes
related
phytochelatins
(PCS)
metallothioneins
(MT),
antioxidant
system
(GS
GR),
transporters
(COPT1).
The
results
indicated
both
were
accumulated
mainly
roots,
339.9
433.8
mg/kg
DW,
Cu,
respectively,
exhibiting
excluder
pattern.
increased
length
principal
root.
Higher
doses
augmented
content
(62.8%
41.2%
compared
control,
respectively).
GS,
GR,
COPT1
modified
transcriptional
levels
depending
organ
evaluated.
These
provide
evidence
specific
this
against
heavy
stress,
which
helpful
guide
new
research
efforts
support
development
strategies
using
phytoremediation.
Язык: Английский
Multiblock NIR and MIR spectralprint through AComDim to evaluate the effects of growing site, harvest season, and clone on yerba mate leaves composition
Food Chemistry,
Год журнала:
2025,
Номер
477, С. 143459 - 143459
Опубликована: Фев. 21, 2025
Язык: Английский
Geographical variations in Polygonatum polysaccharides and saponins biosynthesis in Polygonatum species
Industrial Crops and Products,
Год журнала:
2025,
Номер
227, С. 120814 - 120814
Опубликована: Март 6, 2025
Язык: Английский
A systematic review on the yerba mate (Ilex paraguariensis A. St. Hil.)
Journal of Food Composition and Analysis,
Год журнала:
2025,
Номер
unknown, С. 107466 - 107466
Опубликована: Март 1, 2025
Язык: Английский
Metabolite Profiling and Identification of Sweet/Bitter Taste Compounds in the Growth of Cyclocarya Paliurus Leaves Using Multiplatform Metabolomics
Foods,
Год журнала:
2024,
Номер
13(19), С. 3089 - 3089
Опубликована: Сен. 27, 2024
Cyclocarya
paliurus
tea,
also
known
as
“sweet
tea”,
an
herbal
tea
with
leaves
raw
material,
is
famous
for
its
unique
nutritional
benefits
and
flavor.
However,
due
to
the
“bittersweet”
of
it
still
unable
fully
satisfy
consumers’
high-quality
taste
experience
satisfaction.
Therefore,
this
study
aimed
explore
metabolites
in
during
their
growth
period,
particularly
composition
variation
sweet
bitter
compounds,
by
combining
multi-platform
metabolomics
analysis
electronic
tongue
system
molecular
docking
simulation
technology.
The
results
indicated
that
there
were
significant
differences
contents
total
phenols,
flavonoids,
polysaccharides,
saponins
C.
different
growing
months.
A
575
secondary
identified
potential
active
related
sweet/bitter
using
nontargeted
based
on
UHPLC-MS/MS
analysis.
Moreover,
technology
was
utilized
interactions
between
candidate
receptors
T1R2/T1R3
T2R4/T2R14.
Six
key
compounds
high
sweetness
low
bitterness
successfully
computational
analysis,
including
cis-anethole,
gluconic
acid,
beta-D-Sedoheptulose,
asparagine,
proline,
citrulline,
which
may
serve
candidates
modification
leaves.
These
findings
provide
a
new
perspective
understanding
characteristics
contribute
distinctive
sensory
quality
Язык: Английский