Elucidating the Impact ofHypericum alpestreExtract and L-NAME on the PI3K/Akt Signaling Pathway in A549 Lung Adenocarcinoma Cells DOI Creative Commons
Hayarpi Javrushyan, Mikayel Ginovyan,

Gayane Petrosyan

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 4, 2024

Abstract Plants within the Hypericaceae family have been traditionally used for their medicinal properties, showcasing a wide range of effects such as antibacterial, antiviral, and antioxidant qualities. Hypericum alpestre (HA) extracts exhibited significant cytotoxicity against various cancer cell lines. The phenolic compounds found in HA attracted attention potential prevention. L-NAME, known its ability to inhibit nitric oxide synthase (NOS) activity, has emerged promising approach therapy. However, precise molecular mechanisms underlying anticancer L-NAME remain unclear. This study aims clarify impact on PI3K/Akt signaling pathway A549 lung adenocarcinoma cells, with specific focus TNFa/COX-2 VEGFa/MMP-2 pathways. In silico analysis, they identified highest affinity PI3K/Akt, finding validated by subsequent vitro experiments. Furthermore, combination herbs superior efficacy compared herb 5-FU combination, evidenced promotion apoptosis. Both alone demonstrated inhibitory therapeutic is hypothesized operate through PI3k/Akt pathway. A better understanding interaction between polyphenols could pave way novel strategies cancer, including drug-resistant tumors.

Язык: Английский

Exploring the medicinal significance of l-Arginine mediated nitric oxide in preventing health disorders DOI Creative Commons
Mega Obukohwo Oyovwi, Adedeji David Atere

European Journal of Medicinal Chemistry Reports, Год журнала: 2024, Номер 12, С. 100175 - 100175

Опубликована: Июнь 15, 2024

l-Arginine is an essential amino acid that plays a crucial role in various physiological processes. It serves as precursor for nitric oxide (NO), which has potent antioxidant and anti-inflammatory properties. This review aims to comprehensively examine the medicinal importance of l-arginine natural preventing human health disorders. A comprehensive literature search was conducted using PubMed, Google Scholar, other databases. Studies investigating effects its potential diseases were included. been shown mediate NO production with strong properties, scavenging free radicals reducing oxidative stress. demonstrated therapeutic mitigating conditions, including: Cardiovascular diseases, Neurodegenerative Metabolic disorders, Immune function Anti-aging effects. significant importance. Its ability scavenge radicals, improve endothelial function, support immune makes it promising agent treating wide range Further research warranted fully elucidate mechanisms action optimal dosage specific conditions.

Язык: Английский

Процитировано

18
Royal Jelly–Mediated Silver Nanoparticles Show Promising Anticancer Effect on HeLa and A549 Cells Through Modulation of the VEGFa/PI3K/Akt/MMP‐2 Pathway DOI

Meri Kocharyan,

Syuzan Marutyan, Edita Nadiryan

и другие.

Applied Organometallic Chemistry, Год журнала: 2024, Номер 38(12)

Опубликована: Авг. 27, 2024

ABSTRACT In recent years, nanotechnology has revolutionized various sectors, particularly in nanomedicine, where nanomaterials are used for diagnosis, monitoring, control, prevention, and treatment. Among these, silver nanoparticles (AgNPs) stand out due to their remarkable antimicrobial cytotoxic properties. Traditional chemical synthesis of AgNPs poses significant environmental health risks. This study introduces a novel, eco‐friendly method using royal jelly (RJ), nutrient‐rich secretion from honeybees, produce with potent anticancer effects. Our research provides detailed investigation into RJ‐mediated AgNPs’ modulation the VEGFa/PI3K/Akt/MMP‐2 pathway HeLa A549 cancer cell lines. AgNP characterization was performed by applying UV–Vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM) combination selected area diffraction (SAED). Cell signaling system components were determined ELISA analysis. The cells subjected staining hematoxylin eosin visualize treatment’s focuses on green RJ its bioactivity against cells. It examines effects cells, specifically cervical lung Green synthesized demonstrate underlying molecular mechanisms contributing activity elucidated. findings revealed decrease arginase upon exposure AgNPs, accompanied reductions PI3K phosphorylated total Akt levels, indicative inhibition. Additionally, RJAgNP demonstrated capacity reduce nitric oxide levels suppress angiogenesis‐related factors like VEGF MMP‐2 inflammation‐related TNF‐α COX‐2, thus impeding angiogenesis metastasis. Moreover, our results shed involvement reactive oxygen intermediates (ROIs) mediating apoptotic pathways, as evidenced increase malondialdehyde (MDA) concentration corresponding ultimately promoting death contributes significantly field nanomedicine therapy introducing use providing in‐depth insights action. potential RJ‐AgNPs targeting ROS/RNS

Язык: Английский

Процитировано

3
Elucidating the impact of Hypericum alpestre extract and L-NAME on the PI3K/Akt signaling pathway in A549 lung adenocarcinoma and MDA-MB-231 triple-negative breast cancer cells DOI Creative Commons
Hayarpi Javrushyan, Mikayel Ginovyan, Tigran Harutyunyan

и другие.

PLoS ONE, Год журнала: 2025, Номер 20(4), С. e0303736 - e0303736

Опубликована: Апрель 3, 2025

Plants of the Hypericaceae family have been traditionally used for their medicinal properties, including antibacterial, antiviral, and antioxidant activities. Among these, Hypericum alpestre (HA) extracts shown notable cytotoxicity against various cancer cell lines, drawing attention to phenolic compounds as potential anticancer agents. Similarly, N(G)-Nitro-L-arginine methyl ester (L-NAME), an inhibitor nitric oxide synthase (NOS) activity, has emerged a promising candidate in therapy. However, precise molecular mechanisms underlying effects both HA L-NAME remain unclear. This study aimed clarify impact on phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/ mammalian target rapamycin (mTOR) signaling pathway A549 human lung adenocarcinoma MDA-MB-231 breast triple-negative cells, with particular emphasis tumor necrosis factor-alpha (TNFα)/cyclooxygenase-2 (COX-2) vascular endothelial growth factor A (VEGFα)/matrix metalloproteinase-2 (MMP-2) pathways. In silico analyses identified within highest affinity PI3K/Akt, finding subsequently confirmed by vitro experiments. Notably, combination demonstrated greater efficacy than 5-fluorouracil (5-FU), evidenced enhanced apoptotic activity. Both alone inhibited TNFα/COX-2 VEGFα/MMP-2 These results suggest that therapeutic HA, especially L-NAME, may be mediated through PI3K/Akt pathway. better understanding interaction between polyphenols could pave way novel strategies cancer, drug-resistant tumors.

Язык: Английский

Процитировано

0
Novel Hypericin-loaded nanoparticles as a new drug delivery system ameliorate olanzapine-induced early renal injury and oxidative stress: In vitro and in vivo approaches DOI Creative Commons
Abeer M. Abd El-Hameed,

Fatema A. AbdelAlim,

Amal H. Alsehli

и другие.

Results in Chemistry, Год журнала: 2025, Номер unknown, С. 102233 - 102233

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
L-Arginine and Immune Modulation: A Pharmacological Perspective on Inflammation and Autoimmune Disorders DOI
Igbayilola Yusuff Dimeji,

Kasim Sakran Abass,

Ngabea Murtala Audu

и другие.

European Journal of Pharmacology, Год журнала: 2025, Номер unknown, С. 177615 - 177615

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Impact of Immobilization Stress on Erythropoiesis, Alterations in the Antioxidant System, and Potential Correction with Stevia rebaudiana Bertoni in Rabbit DOI Creative Commons
Hasmik Karapetyan,

Ernestina Kh. Barseghyan,

Anna Karapetyan

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Март 7, 2024

Abstract This study investigates the impact of immobilization stress as a risk factor for pathological processes, with focus on erythropoiesis and oxidative stress. Additionally, potential therapeutic effects Stevia rebaudiana Bertoni in mitigating these stress-induced alterations are explored. Immobilization was induced 30 days, its antioxidant systems were assessed. administered to evaluate preventing correcting observed negative processes. Prolonged led disruptions erythropoiesis, manifested by delay proliferative maturation processes moderate decrease erythrocyte equilibrium. Concurrently, increased duration stimulated lipid peroxidation inhibited enzyme activity. The use demonstrated significant positive restored activity enzymes, particularly peroxidase catalase. adversely affects balance, contributing emerges promising intervention, displaying attenuate through influence mechanisms.

Язык: Английский

Процитировано

0
Royal Jelly-Mediated Silver Nanoparticles Show Promising Anti-Cancer Effect on HeLa and A549 Cells Through Modulation of the VEGFa/PI3K/Akt/MMP-2 Pathway DOI Creative Commons

Meri Kocharyan,

Syuzan Marutyan, Edita Nadiryan

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Март 11, 2024

Abstract Cancer poses a significant challenge in the medical field, requiring thorough investigation into its mechanisms and development of effective treatments. Recently, there has been increasing interest integrating drugs with metal nanoparticles, which are notable for their unique size physicochemical properties, aiming to enhance anticancer efficacy. Silver nanoparticles (AgNPs), especially those obtained through environmentally friendly methods known as green synthesis, have garnered attention. Royal jelly (RJ), substance produced by bees recognized antioxidant, anti-inflammatory, antibacterial is particularly interesting. This study focuses on synthesis AgNPs using royal bioactivity against cancer cells. It provides detailed characterization examines effects cells, specifically Hela cervical A549 lung cell lines. The results highlight cytotoxicity induced HeLa mediated apoptosis via PI3K/AKT signaling pathway. Our research findings demonstrate that one underlying death involves increased concentration ROS/RNS downregulation VEGFa/MMP-2/COX-2 among few elucidate mechanism synthesized this method. Overall, our contributes ongoing exploration biology offers insights potential therapeutic approaches harnessing capabilities green-synthesized nanoparticles.

Язык: Английский

Процитировано

0
Elucidating the Impact ofHypericum alpestreExtract and L-NAME on the PI3K/Akt Signaling Pathway in A549 Lung Adenocarcinoma Cells DOI Creative Commons
Hayarpi Javrushyan, Mikayel Ginovyan,

Gayane Petrosyan

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 4, 2024

Abstract Plants within the Hypericaceae family have been traditionally used for their medicinal properties, showcasing a wide range of effects such as antibacterial, antiviral, and antioxidant qualities. Hypericum alpestre (HA) extracts exhibited significant cytotoxicity against various cancer cell lines. The phenolic compounds found in HA attracted attention potential prevention. L-NAME, known its ability to inhibit nitric oxide synthase (NOS) activity, has emerged promising approach therapy. However, precise molecular mechanisms underlying anticancer L-NAME remain unclear. This study aims clarify impact on PI3K/Akt signaling pathway A549 lung adenocarcinoma cells, with specific focus TNFa/COX-2 VEGFa/MMP-2 pathways. In silico analysis, they identified highest affinity PI3K/Akt, finding validated by subsequent vitro experiments. Furthermore, combination herbs superior efficacy compared herb 5-FU combination, evidenced promotion apoptosis. Both alone demonstrated inhibitory therapeutic is hypothesized operate through PI3k/Akt pathway. A better understanding interaction between polyphenols could pave way novel strategies cancer, including drug-resistant tumors.

Язык: Английский

Процитировано

0