BMC Research Notes,
Год журнала:
2025,
Номер
18(1)
Опубликована: Апрель 8, 2025
Understanding
the
regulation
and
function
of
plant
genes
is
essential
for
addressing
challenges
faced
by
modern
agriculture.
Plant
transformation,
in
conjunction
with
fluorescence
microscopy,
offers
a
powerful
approach
to
investigate
dynamic
behavior
proteins
they
encode.
We
previously
developed
set
Gateway-compatible
tissue-specific
transformation
vectors.
In
this
paper
we
aim
expand
toolkit
vectors
available
Agrobacterium-mediated
protoplast
transfection.
Here,
introduce
new
introducing
additional
fluorophores
create
pJRA
vector
series.
Additionally,
pLCS
series
vectors,
modular
Gateway-
Gibson
assembly-compatible
designed
All
described
are
from
Addgene
serve
as
resource
research
community.
Bioconjugate Chemistry,
Год журнала:
2021,
Номер
32(12), С. 2457 - 2479
Опубликована: Ноя. 30, 2021
Bioorthogonal
chemistry
is
a
set
of
methods
using
the
non-native
functional
groups
to
explore
and
understand
biology
in
living
organisms.
In
this
review,
we
summarize
most
common
reactions
used
bioorthogonal
methods,
their
relative
advantages
disadvantages,
frequency
occurrence
published
literature.
We
also
briefly
discuss
some
less
but
potentially
useful
methods.
then
analyze
bioorthogonal-related
publications
CAS
Content
Collection
determine
how
often
different
types
biomolecules
such
as
proteins,
carbohydrates,
glycans,
lipids
have
been
studied
chemistry.
The
prevalent
biological
chemical
for
attaching
these
are
elaborated.
publication
volume
related
applications
Collection.
use
imaging,
identifying,
characterizing
delivering
drugs
treat
disease
discussed
at
length.
surface
attachment
proteins
modified
carbohydrates
noted.
Finally,
state
art
its
current
limitations
promise
future
productive
biology.
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(5), С. 3075 - 3083
Опубликована: Янв. 30, 2023
The
specific
and
covalent
labeling
of
the
protein
HaloTag
with
fluorescent
probes
in
living
cells
makes
it
a
powerful
tool
for
bioimaging.
However,
irreversible
attachment
probe
to
precludes
imaging
applications
that
require
transient
binding
comes
risk
photobleaching.
Here,
we
introduce
exchangeable
ligands
fluorescence
(xHTLs)
reversibly
bind
can
be
coupled
rhodamines
different
colors.
In
stimulated
emission
depletion
(STED)
microscopy,
exchange
xHTLs
allows
reduced
photobleaching
as
compared
labeling.
Transient
fluorogenic
fusion
proteins
enables
points
accumulation
nanoscale
topography
(PAINT)
MINFLUX
microscopy.
We
furthermore
pairs
mutants
dual-color
PAINT
STED
thus
open
up
new
possibilities
across
microscopy
platforms
widely
used
approach.
Chemical Society Reviews,
Год журнала:
2023,
Номер
52(20), С. 7197 - 7261
Опубликована: Янв. 1, 2023
In
the
realm
of
biological
research,
invention
super-resolution
microscopy
(SRM)
has
enabled
visualization
ultrafine
sub-cellular
structures
and
their
functions
in
live
cells
at
nano-scale
level,
beyond
diffraction
limit,
which
opened
up
a
new
window
for
advanced
biomedical
studies
to
unravel
complex
unknown
details
physiological
disorders
level
with
unprecedented
resolution
clarity.
However,
most
SRM
techniques
are
highly
reliant
on
personalized
special
photophysical
features
fluorophores.
recent
times,
there
been
an
surge
development
robust
fluorophore
systems
various
imaging
techniques.
To
date,
xanthene,
cyanine,
oxazine
BODIPY
cores
have
authoritatively
utilized
as
basic
units
small-molecule-based
organic
fluorescent
probe
designing
strategies
owing
excellent
characteristics
easy
synthetic
acquiescence.
Since
future
next-generation
will
be
decided
by
availability
probes
these
four
building
blocks
play
important
role
progressive
design,
is
urgent
need
review
advancements
fluorophores
different
methods
based
dye
cores.
This
article
not
only
includes
comprehensive
discussion
about
developments
emphasis
effective
integration
into
cell
bio-imaging
applications
but
also
critically
evaluates
background
each
highlight
merits
demerits
towards
developing
newer
SRM.
Chemical Reviews,
Год журнала:
2024,
Номер
124(10), С. 6198 - 6270
Опубликована: Май 8, 2024
Hybrid
small-molecule/protein
fluorescent
probes
are
powerful
tools
for
visualizing
protein
localization
and
function
in
living
cells.
These
hybrid
constructed
by
diverse
site-specific
chemical
labeling
approaches
through
reactions
to
exogenous
peptide/small
tags,
enzymatic
post-translational
modifications,
bioorthogonal
genetically
incorporated
unnatural
amino
acids,
ligand-directed
reactions.
The
employed
imaging
trafficking,
conformational
changes,
bioanalytes
surrounding
proteins.
In
addition,
facilitate
visualization
of
dynamics
at
the
single-molecule
level
defined
structure
with
super-resolution
imaging.
this
review,
we
discuss
development
bioimaging
applications
based
on
hybrids.
Molecules,
Год журнала:
2025,
Номер
30(4), С. 939 - 939
Опубликована: Фев. 18, 2025
Enzyme
immobilization
plays
a
critical
role
in
enhancing
the
efficiency
and
sustainability
of
biocatalysis,
addressing
key
challenges
such
as
limited
enzyme
stability,
short
shelf
life,
difficulties
recovery
recycling,
which
are
pivotal
for
green
chemistry
industrial
applications.
Classical
approaches,
including
adsorption,
entrapment,
encapsulation,
covalent
bonding,
well
advanced
site-specific
methods
that
integrate
engineering
bio-orthogonal
chemistry,
were
discussed.
These
techniques
enable
precise
control
over
orientation
interaction
with
carriers,
optimizing
catalytic
activity
reusability.
Key
findings
highlight
impact
on
improving
performance
under
various
operational
conditions
its
reducing
process
costs
through
enhanced
stability
recyclability.
The
review
presents
numerous
practical
applications
immobilized
enzymes,
their
use
pharmaceutical
industry
drug
synthesis,
food
sector
dairy
processing,
environmental
biotechnology
wastewater
treatment
dye
degradation.
Despite
significant
advantages,
loss
due
to
conformational
changes
mass
transfer
limitations
remain,
necessitating
tailored
protocols
specific
integration
modern
biotechnological
advancements,
site-directed
mutagenesis
recombinant
DNA
technology,
offers
promising
pathway
developing
robust,
efficient,
sustainable
biocatalytic
systems.
This
comprehensive
guide
aims
support
researchers
industries
selecting
diverse
pharmaceuticals,
fine
chemicals.
Neuron,
Год журнала:
2022,
Номер
110(24), С. 4057 - 4073.e8
Опубликована: Окт. 5, 2022
The
lifetime
of
proteins
in
synapses
is
important
for
their
signaling,
maintenance,
and
remodeling,
memory
duration.
We
quantified
the
endogenous
PSD95,
an
abundant
postsynaptic
protein
excitatory
synapses,
at
single-synapse
resolution
across
mouse
brain
lifespan,
generating
Protein
Lifetime
Synaptome
Atlas.
Excitatory
have
a
wide
range
PSD95
lifetimes
extending
from
hours
to
several
months,
with
distinct
spatial
distributions
dendrites,
neurons,
regions.
Synapses
short
are
enriched
young
animals
regions
controlling
innate
behaviors,
whereas
long
accumulate
during
development,
cortex
CA1
where
memories
stored,
preferentially
preserved
old
age.
Synapse
increases
throughout
model
autism
schizophrenia.
adds
further
layer
synapse
diversity
enriches
prevailing
concepts
aging,
disease.
Abstract
Visualizing
the
structure
and
dynamics
of
biomolecules
is
critical
to
understand
biological
function,
requires
methods
fluorescently
label
targets
interest
in
their
cellular
context.
Self‐labelling
proteins,
which
combine
a
genetically
encoded
tag
with
small‐molecule
fluorophore,
have
attracted
considerable
attention
for
this
purpose,
as
they
can
overcome
limitations
fluorescent
proteins.
Among
them,
HaloTag
protein
most
broadly
used,
showing
fast
specific
labelling
small,
easy
functionalize
cell‐permeant
ligand.
Synthetic
chemistry
engineering
provided
portfolio
powerful
imaging
tools
exploiting
HaloTag,
along
general
optimize
adapt
them
applications.
Here,
we
provide
an
overview
reporters
based
on
biosensing,
highlighting
strategies
