Expanding the Promiscuity of a Copper‐Dependent Oxidase for Enantioselective Cross‐Coupling of Indoles

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(16)

Опубликована: Фев. 15, 2023

Herein, we disclose the highly enantioselective oxidative cross-coupling of 3-hydroxyindole esters with various nucleophilic partners as catalyzed by copper efflux oxidase. The biocatalytic transformation delivers functionalized 2,2-disubstituted indolin-3-ones excellent optical purity (90-99 % ee), which exhibited anticancer activity against MCF-7 cell lines, shown preliminary biological evaluation. Mechanistic studies and molecular docking results suggest formation a phenoxyl radical enantiocontrol facilitated suited enzyme chiral pocket. This study is significant regard to expanding catalytic repertoire natural multicopper oxidases well enlarging synthetic toolbox for sustainable asymmetric coupling.

Язык: Английский

---

Improving Enzyme Fitness with Machine Learning

CHIMIA International Journal for Chemistry, Год журнала: 2023, Номер 77(3), С. 116 - 116

Опубликована: Март 29, 2023

The combinatorial composition of proteins has triggered the application machine learning in enzyme engineering. By predicting how protein sequence encodes function, researchers aim to leverage models select a reduced number optimized sequences for laboratory measurement with lower costs and shorten timelines engineering campaigns. In this review, we will highlight successful algorithm-aided examples, including work carried out within NCCR Catalysis. context, discuss underlying computational methods developed improve properties such as enantioselectivity, regioselectivity, activity, stability. Considering rapid maturing techniques, expect that their continued campaigns be key deliver additional powerful biocatalysts sustainable chemical synthesis.

Язык: Английский

---

Metalloenzyme-inspired approach to the design and applications of phosphatase-mimetic nanozymes. Bridging the inorganic and organic worlds

Environmental Science Nano, Год журнала: 2024, Номер 11(8), С. 3268 - 3285

Опубликована: Янв. 1, 2024

Both nanozymes (cerium oxide) and natural enzymes can destroy toxic organophosphates (pesticide paraoxon ethyl) using analogous reaction mechanisms.

Язык: Английский

---

Advancing sustainable biotechnology through protein engineering

Trends in Biochemical Sciences, Год журнала: 2024, Номер 49(11), С. 955 - 968

Опубликована: Сен. 3, 2024

Язык: Английский

---

Enriching productive mutational paths accelerates enzyme evolution

Nature Chemical Biology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 11, 2024

Язык: Английский

---