Investigation for Regulation of a DNA-Programmed Bimetallic Nanozyme and Its Biosensing Applications DOI

Jiaxuan Xiao,

Xiaofeng Yang,

Xinshuo Zhang

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(49), С. 19796 - 19802

Опубликована: Ноя. 30, 2024

The DNA-mediated growth strategy of bimetallic nanozymes is considered as an effective approach to regulate their peroxidase activity via tuning the morphology and nanostructure. Albeit important, its biosensing application in rational methods' design performance improvement limited due deficiency a systematic understanding interactions between DNA nanomaterials used. Herein, four homo-oligonucleotides capping ligands were employed functionalize nanozymes, where Pt nanoparticles (PtNPs) situ synthesized onto DNA-bound Au nanorods (AuNRs), effects with different lengths on state investigated detail. It was found that aggregation AuNRs obviously depended variety number oligonucleotides absorbance ratio at 810 525 nm (A810/A525), ranking follows: AuNRs/A10/PtNPs > AuNRs/G10/PtNPs AuNRs/C10/PtNPs ≫ AuNRs/T10/PtNPs, which consistent value Km for TMB, indicating dispersal/aggregation closely related deposition PtNPs, thereby significantly influencing activity. According our discoveries, novel colorimetric array platform fabricated using above types DNA-encoded Pt–Au sensing elements sensitively discriminating five biological thiols (l-cys, GSH, Hcy, DTT, Cys–Gly) identifying normal cells/tumor cells, respectively. Our work provides new insight into DNA-programmed nanozyme regulation broadens applications.

Язык: Английский

Investigation for Regulation of a DNA-Programmed Bimetallic Nanozyme and Its Biosensing Applications DOI

Jiaxuan Xiao,

Xiaofeng Yang,

Xinshuo Zhang

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(49), С. 19796 - 19802

Опубликована: Ноя. 30, 2024

The DNA-mediated growth strategy of bimetallic nanozymes is considered as an effective approach to regulate their peroxidase activity via tuning the morphology and nanostructure. Albeit important, its biosensing application in rational methods' design performance improvement limited due deficiency a systematic understanding interactions between DNA nanomaterials used. Herein, four homo-oligonucleotides capping ligands were employed functionalize nanozymes, where Pt nanoparticles (PtNPs) situ synthesized onto DNA-bound Au nanorods (AuNRs), effects with different lengths on state investigated detail. It was found that aggregation AuNRs obviously depended variety number oligonucleotides absorbance ratio at 810 525 nm (A810/A525), ranking follows: AuNRs/A10/PtNPs > AuNRs/G10/PtNPs AuNRs/C10/PtNPs ≫ AuNRs/T10/PtNPs, which consistent value Km for TMB, indicating dispersal/aggregation closely related deposition PtNPs, thereby significantly influencing activity. According our discoveries, novel colorimetric array platform fabricated using above types DNA-encoded Pt–Au sensing elements sensitively discriminating five biological thiols (l-cys, GSH, Hcy, DTT, Cys–Gly) identifying normal cells/tumor cells, respectively. Our work provides new insight into DNA-programmed nanozyme regulation broadens applications.

Язык: Английский

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