Frontiers in Pharmacology,
Год журнала:
2022,
Номер
13
Опубликована: Авг. 8, 2022
A
wound
occurs
when
the
epidermis
and
dermis
of
skin
are
damaged
internally
externally.
The
traditional
healing
method
is
unsatisfactory,
which
will
prolong
treatment
time
increase
cost,
brings
economic
psychological
burdens
to
patients.
Therefore,
there
an
urgent
need
for
a
new
accelerate
healing.
As
cell-free
therapy,
exosome
derived
from
stem
cell
(EdSC)
offers
possibilities
EdSC
smallest
extracellular
vesicle
secreted
by
cells
with
diameters
30-150
nm
lipid
bilayer
structure.
Previous
studies
have
found
that
can
participate
in
promote
almost
all
stages
healing,
including
regulating
inflammatory
cells;
improving
activation
fibroblasts,
keratinocytes,
endothelial
adjusting
ratio
collagen
Ⅰ
Ⅲ.
We
reviewed
relevant
knowledge
wounds;
summarized
biogenesis,
isolation,
identification
exosomes;
clarified
pharmacological
role
exosomes
promoting
This
review
provides
support
study
exosomes.
Journal of Nanobiotechnology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Дек. 20, 2023
Exosomes
are
extracellular
vesicles
with
the
diameter
of
30
~
150
nm,
and
widely
involved
in
intercellular
communication,
disease
diagnosis
drug
delivery
carriers
for
targeted
therapy.
Therapeutic
application
exosomes
as
is
limited
due
to
lack
sources
methods
obtaining
adequate
exosomes.
Milk
contains
abundant
exosomes,
several
studies
have
shown
that
milk-derived
play
crucial
roles
preventing
treating
intestinal
diseases.
In
this
review,
we
summarized
biogenesis,
secretion
structure,
current
novel
used
extraction
identification
well
discussed
role
diseases,
such
inflammatory
bowel
disease,
necrotizing
enterocolitis,
colorectal
cancer,
ischemia
reperfusion
injury
by
regulating
immune
homeostasis,
restoring
gut
microbiota
composition
improving
structure
integrity,
alleviating
conditions
oxidative
stress,
cell
apoptosis
inflammation,
reducing
mitochondrial
reactive
oxygen
species
(ROS)
lysosome
accumulation
both
humans
animals.
addition,
future
prospects
standardization
milk
exosome
production
platform
obtain
higher
concentration
purity,
complete
derived
from
milk.
Several
vivo
clinical
needed
establish
an
effective
efficient
system,
promote
its
treatment
various
diseases
Critical Reviews in Food Science and Nutrition,
Год журнала:
2025,
Номер
unknown, С. 1 - 21
Опубликована: Март 19, 2025
Exosomes
are
nanoscale
particles
with
a
lipid
bilayer
membrane
that
were
first
identified
in
mammalian
cells.
Plant-derived
exosome-like
nanoparticles
(PELNs)
share
structural
and
molecular
similarities
exosomes,
including
lipids,
proteins,
microRNA
(miRNA),
plant-derived
metabolites.
Owing
to
their
unique
characteristics,
such
as
outstanding
stability,
low
immunogenicity,
high
biocompatibility,
sustainability,
PELNs
have
emerged
promising
natural
bioactive
agents
the
capacity
for
cross-kingdom
cellular
regulation.
Dietary
supplementation
PELNs,
particularly
from
fruits
vegetables,
has
demonstrated
health
benefits.
An
increasing
number
of
studies
indicated
beneficial
effects
on
colon
health.
This
review
summarizes
isolation
characterization
uptake,
distribution
after
oral
ingestion.
Furthermore,
this
emphasizes
interactions
between
gut
microbiota,
immune
system,
uptake
by
modulation
bacteria
metabolism,
responses
PELNs.
Additionally,
applications
components
drug
carriers
targeting
reviewed.
In
summary,
represent
versatile
approach
improve
health,
potential
both
therapeutic
preventive
healthcare
strategies.
Nanoscale,
Год журнала:
2022,
Номер
14(25), С. 8995 - 9003
Опубликована: Янв. 1, 2022
Exosomes,
a
subgroup
of
extracellular
vesicles
secreted
by
multiple
cells,
have
great
potential
as
cancer
biomarkers
in
clinical
applications.
However,
enrichment
and
detection
exosomes
from
complex
media
remain
huge
challenge
due
to
their
small
size.
Herein,
we
used
iodixanol
density
gradient
centrifugation
for
the
isolation
purification
label-free
exosomal
PD-L1
using
biochip
based
on
surface
plasmon
resonance
(SPR-ExoPD-L1).
The
obtained
are
lipid-bilayer
classical
exosome
markers
CD9,
CD63
CD81
highly
enriched.
Besides,
is
specifically
expressed
instead
non-vesicular
components
or
large
vesicles.
Compared
with
enzyme-linked
immunosorbent
assays,
SPR-ExoPD-L1
assay
could
better
distinguish
derived
melanoma
cells
different
levels
PD-L1.
Accurate
measurement
provide
critical
information
diagnosis
personalized
immunotherapy
cancer.
Frontiers in Pharmacology,
Год журнала:
2022,
Номер
13
Опубликована: Авг. 8, 2022
A
wound
occurs
when
the
epidermis
and
dermis
of
skin
are
damaged
internally
externally.
The
traditional
healing
method
is
unsatisfactory,
which
will
prolong
treatment
time
increase
cost,
brings
economic
psychological
burdens
to
patients.
Therefore,
there
an
urgent
need
for
a
new
accelerate
healing.
As
cell-free
therapy,
exosome
derived
from
stem
cell
(EdSC)
offers
possibilities
EdSC
smallest
extracellular
vesicle
secreted
by
cells
with
diameters
30-150
nm
lipid
bilayer
structure.
Previous
studies
have
found
that
can
participate
in
promote
almost
all
stages
healing,
including
regulating
inflammatory
cells;
improving
activation
fibroblasts,
keratinocytes,
endothelial
adjusting
ratio
collagen
Ⅰ
Ⅲ.
We
reviewed
relevant
knowledge
wounds;
summarized
biogenesis,
isolation,
identification
exosomes;
clarified
pharmacological
role
exosomes
promoting
This
review
provides
support
study
exosomes.
