Frontiers in Pharmacology,
Год журнала:
2022,
Номер
13
Опубликована: Авг. 8, 2022
A
wound
occurs
when
the
epidermis
and
dermis
of
skin
are
damaged
internally
externally.
The
traditional
healing
method
is
unsatisfactory,
which
will
prolong
treatment
time
increase
cost,
brings
economic
psychological
burdens
to
patients.
Therefore,
there
an
urgent
need
for
a
new
accelerate
healing.
As
cell-free
therapy,
exosome
derived
from
stem
cell
(EdSC)
offers
possibilities
EdSC
smallest
extracellular
vesicle
secreted
by
cells
with
diameters
30-150
nm
lipid
bilayer
structure.
Previous
studies
have
found
that
can
participate
in
promote
almost
all
stages
healing,
including
regulating
inflammatory
cells;
improving
activation
fibroblasts,
keratinocytes,
endothelial
adjusting
ratio
collagen
Ⅰ
Ⅲ.
We
reviewed
relevant
knowledge
wounds;
summarized
biogenesis,
isolation,
identification
exosomes;
clarified
pharmacological
role
exosomes
promoting
This
review
provides
support
study
exosomes.
Abstract
Exosomes
are
extracellular
vesicles
that
actively
secreted
by
most
live
cells
via
endosome
pathway.
They
distribute
ubiquitously
in
body
fluids
and
play
important
roles
physiological
pathological
processes.
Recent
studies
have
revealed
exosomes
carry
cell‐specific
cargoes,
such
as
proteins
nucleic
acids,
which
been
recognized
emerging
noninvasive
biomarkers
liquid
biopsy.
Therefore,
the
molecular
profiling
of
tumor‐derived
is
crucial
facilitating
discovery
cancer‐related
biomarkers.
Recently,
various
techniques
proposed
for
sensitive,
accurate,
rapid
characterization
these
small
vesicles.
To
meet
growing
demands
multiplexed
detection,
microfluidics
has
integrated
with
diverse
traditional
analytical
methods
powerful
components
analysis.
Taking
advantages
their
inherent
merits
great
sensitivity,
high
analysis
performance
system
integration,
short
time,
low
cost,
volume
sample,
improved
potential
control,
automation,
disposability,
microfluidic
systems
significantly
exosomes.
In
this
review,
we
summarized
recent
progresses
technologies
exosomes,
including
proteins,
metabolites,
lipids.
Furthermore,
discussed
major
challenges
offered
a
perspective
strategy‐based
exosomal
discovery.
We
expected
in‐depth
summary
devices–assisted
will
greatly
facilitate
biomarkers,
thereby
realizing
highly
efficient
accurate
diagnosis,
prediction
cancers.
Moreover,
view
significance
interesting
approach
exosomes‐based
biopsy,
review
contribute
to
translation
technology
into
clinical
applications.
Journal of Nanobiotechnology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Дек. 20, 2023
Exosomes
are
extracellular
vesicles
with
the
diameter
of
30
~
150
nm,
and
widely
involved
in
intercellular
communication,
disease
diagnosis
drug
delivery
carriers
for
targeted
therapy.
Therapeutic
application
exosomes
as
is
limited
due
to
lack
sources
methods
obtaining
adequate
exosomes.
Milk
contains
abundant
exosomes,
several
studies
have
shown
that
milk-derived
play
crucial
roles
preventing
treating
intestinal
diseases.
In
this
review,
we
summarized
biogenesis,
secretion
structure,
current
novel
used
extraction
identification
well
discussed
role
diseases,
such
inflammatory
bowel
disease,
necrotizing
enterocolitis,
colorectal
cancer,
ischemia
reperfusion
injury
by
regulating
immune
homeostasis,
restoring
gut
microbiota
composition
improving
structure
integrity,
alleviating
conditions
oxidative
stress,
cell
apoptosis
inflammation,
reducing
mitochondrial
reactive
oxygen
species
(ROS)
lysosome
accumulation
both
humans
animals.
addition,
future
prospects
standardization
milk
exosome
production
platform
obtain
higher
concentration
purity,
complete
derived
from
milk.
Several
vivo
clinical
needed
establish
an
effective
efficient
system,
promote
its
treatment
various
diseases
Nanoscale,
Год журнала:
2022,
Номер
14(25), С. 8995 - 9003
Опубликована: Янв. 1, 2022
Exosomes,
a
subgroup
of
extracellular
vesicles
secreted
by
multiple
cells,
have
great
potential
as
cancer
biomarkers
in
clinical
applications.
However,
enrichment
and
detection
exosomes
from
complex
media
remain
huge
challenge
due
to
their
small
size.
Herein,
we
used
iodixanol
density
gradient
centrifugation
for
the
isolation
purification
label-free
exosomal
PD-L1
using
biochip
based
on
surface
plasmon
resonance
(SPR-ExoPD-L1).
The
obtained
are
lipid-bilayer
classical
exosome
markers
CD9,
CD63
CD81
highly
enriched.
Besides,
is
specifically
expressed
instead
non-vesicular
components
or
large
vesicles.
Compared
with
enzyme-linked
immunosorbent
assays,
SPR-ExoPD-L1
assay
could
better
distinguish
derived
melanoma
cells
different
levels
PD-L1.
Accurate
measurement
provide
critical
information
diagnosis
personalized
immunotherapy
cancer.
Frontiers in Pharmacology,
Год журнала:
2022,
Номер
13
Опубликована: Авг. 8, 2022
A
wound
occurs
when
the
epidermis
and
dermis
of
skin
are
damaged
internally
externally.
The
traditional
healing
method
is
unsatisfactory,
which
will
prolong
treatment
time
increase
cost,
brings
economic
psychological
burdens
to
patients.
Therefore,
there
an
urgent
need
for
a
new
accelerate
healing.
As
cell-free
therapy,
exosome
derived
from
stem
cell
(EdSC)
offers
possibilities
EdSC
smallest
extracellular
vesicle
secreted
by
cells
with
diameters
30-150
nm
lipid
bilayer
structure.
Previous
studies
have
found
that
can
participate
in
promote
almost
all
stages
healing,
including
regulating
inflammatory
cells;
improving
activation
fibroblasts,
keratinocytes,
endothelial
adjusting
ratio
collagen
Ⅰ
Ⅲ.
We
reviewed
relevant
knowledge
wounds;
summarized
biogenesis,
isolation,
identification
exosomes;
clarified
pharmacological
role
exosomes
promoting
This
review
provides
support
study
exosomes.
