Biocatalysis: Enzymatic Synthesis for Industrial Applications

Angewandte Chemie International Edition, Год журнала: 2020, Номер 60(1), С. 88 - 119

Опубликована: Июнь 18, 2020

Abstract Biocatalysis has found numerous applications in various fields as an alternative to chemical catalysis. The use of enzymes organic synthesis, especially make chiral compounds for pharmaceuticals well the flavors and fragrance industry, are most prominent examples. In addition, biocatalysts used on a large scale specialty even bulk chemicals. This review intends give illustrative examples this field with special focus scalable production using enzymes. It also discusses opportunities limitations enzymatic syntheses distinct provides outlook emerging enzyme classes.

Язык: Английский

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Industrial applications of immobilized enzymes—A review

Molecular Catalysis, Год журнала: 2019, Номер 479, С. 110607 - 110607

Опубликована: Сен. 18, 2019

The use of immobilized enzymes is now a routine process for the manufacture many industrial products in pharmaceutical, chemical and food industry. Some enzymes, such as lipases, are naturally robust efficient, can be used production different molecules have wide range applications thanks to their broad selectivity. As an example, lipase from Candida antarctica (CalB) has been by BASF produce chiral compounds, herbicide Dimethenamide-P, which was previously made chemically. enzyme provided significant advantages over process, possibility equimolar concentration substrates, obtain enantiomeric excess > 99%, relatively low temperatures (< 60 °C) organic solvent, single enantiomer instead racemate column configuration that allows dramatic increases productivity. This would not possible without enzyme, since it runs solvent [1]. more specific like transaminases, required protein engineering become suitable APIs (Active Pharmaceutical Ingredients) conditions extreme wild type enzyme. developed Merck sitagliptin good example challenging applied API manufacture. previous involved hydrogenation enamine at high pressure using rhodium-based catalyst. By developing engineered transaminase, enzymatic able convert 200 g/l prositagliptin final product, with e.e. >99.5% presence DMSO cosolvent [2]. For all heterogeneous form brings important environmental advantages, simplified downstream processing or continuous operations. Here, we present series large-scale benefits food, chemical, cosmetics medical device industries, some scarcely reported on previously. In general, reactions benefit immobilization, however, choice them depends economic evaluation costs associated versus obtained process. It concluded rather significant, industry increasing.

Язык: Английский

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Parameters necessary to define an immobilized enzyme preparation

Process Biochemistry, Год журнала: 2019, Номер 90, С. 66 - 80

Опубликована: Ноя. 23, 2019

Язык: Английский

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The Hitchhiker's guide to biocatalysis: recent advances in the use of enzymes in organic synthesis

Chemical Science, Год журнала: 2020, Номер 11(10), С. 2587 - 2605

Опубликована: Янв. 1, 2020

Enzymes are excellent catalysts that increasingly being used in industry and academia. This Perspective provides a general practical guide to enzymes their synthetic potential, primarily aimed at organic chemists.

Язык: Английский

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Enzyme entrapment, biocatalyst immobilization without covalent attachment

Green Chemistry, Год журнала: 2021, Номер 23(14), С. 4980 - 5005

Опубликована: Янв. 1, 2021

The entrapment of enzymes in organic, inorganic or biomaterials can yield active immobilized biocatalysts for chemical transformations without the need to form covalent bonds with protein.

Язык: Английский

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Bridging the gap between transition metal- and bio-catalysis via aqueous micellar catalysis

Nature Communications, Год журнала: 2019, Номер 10(1)

Опубликована: Май 15, 2019

Abstract Previous studies have shown that aqueous solutions of designer surfactants enable a wide variety valuable transformations in synthetic organic chemistry. Since reactions take place within the inner hydrophobic cores these tailor-made nanoreactors, and products made therein are dynamic exchange between micelles through water, opportunities exist to use enzymes effect secondary processes. Herein we report ketone-containing products, formed via initial transition metal-catalyzed based on Pd, Cu, Rh, Fe Au, can be followed same pot by enzymatic reductions mediated alcohol dehydrogenases. Most noteworthy is finding nanomicelles present water appear function not only as medium for both chemo- bio-catalysis, but reservoir substrates, catalysts, decreasing noncompetitive enzyme inhibition.

Язык: Английский

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Round, round we go – strategies for enzymatic cofactor regeneration

Natural Product Reports, Год журнала: 2020, Номер 37(10), С. 1316 - 1333

Опубликована: Янв. 1, 2020

Covering: up to the beginning of 2020Enzymes depending on cofactors are essential in many biosynthetic pathways natural products. They often involved key steps: catalytic conversions that difficult achieve purely with synthetic organic chemistry. Hence, cofactor-dependent enzymes have great potential for biocatalysis, condition a corresponding cofactor regeneration system is available. For some cofactors, these systems require multiple steps; such complex enzyme cascades/multi-enzyme (still) challenging vitro biocatalysis. Further, artificial analogues been synthesised more stable, show an altered reaction range, or act as inhibitors. The development bio-orthogonal can be used production modified products vivo ongoing challenge. In light recent progress this field, review aims provide overview general strategies involving analogues, and systems; highlighting current possibilities application using most common cofactors.

Язык: Английский

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The E factor at 30: a passion for pollution prevention

Green Chemistry, Год журнала: 2023, Номер 25(5), С. 1704 - 1728

Опубликована: Янв. 1, 2023

Publication of the E Factor drew attention to problem waste in chemicals manufacture. Thirty yeas later it is abundantly clear that underlying cause global environmental problems, from climate change plastic pollution.

Язык: Английский

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Recent Advances in the Construction of Phosphorus‐Substituted Heterocycles, 2009–2019

Advanced Synthesis & Catalysis, Год журнала: 2020, Номер 362(9), С. 1724 - 1818

Опубликована: Янв. 20, 2020

Abstract Phosphorus‐substituted heterocycles represent an important class of organophosphorus compounds, which not only widely exist in biologically active pharmaceuticals, agrochemicals, but also have widespread applications material science and organic synthesis as ligands for transition metal complexes. The such compounds integrates the development new synthetic methods exploration efficient catalytic systems, reflects latest achievements organic, metal, photo even electrochemical catalysis, facilitates sufficient quantities related potential medicinal agents biological probes this area. Over past few decades, two major strategies been established toward phosphorus‐substituted heterocycles. This review summarizes recent progress field discusses reaction mechanisms detail. advantages limitations each subdivisional tactic are presented, opportunities still open outlined. magnified image

Язык: Английский

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Biodiesel production from microalgae using lipase-based catalysts: Current challenges and prospects

Algal Research, Год журнала: 2022, Номер 62, С. 102616 - 102616

Опубликована: Янв. 11, 2022

Язык: Английский

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