Genome size variation and whole-genome duplications in the monocot order Poales

Plant Systematics and Evolution, Год журнала: 2025, Номер 311(1)

Опубликована: Янв. 22, 2025

Язык: Английский

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Differential responses of Bradyrhizobium sp. SUTN9-2 to plant extracts and implications for endophytic interactions within different host plants

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 25, 2025

Bradyrhizobium sp. strain SUTN9-2 demonstrates cell enlargement, increased DNA content, and efficient nitrogen fixation in response to rice (Oryza sativa) extract. This is attributed the interaction between plant's cationic antimicrobial peptides (CAMPs) BacA-like transporter (BclA), similar bacteroid legume nodules. The present study reveals that can also establish functional endophytic interactions with chili (Capsicum annuum) tomato (Solanum lycopersicum) plants. When exposed extracts from tomato, exhibits elongation, polyploidy, reduced viability, effects being less pronounced for Transcriptomic cytological analyses revealed genes associated CAMP resistance, metabolism, fixation, defense responses, secretion systems were upregulated, while related cycle certain CAMP-resistance mechanisms downregulated, particularly suggests likely evolves resistance against CAMPs found rice, chili, plants through involving protease-chaperone DegP, AcrAB-TolC multidrug efflux pumps, polysaccharides. These facilitate efflux, degradation, formation of protective barriers resist CAMPs. Such adaptations enable persist colonize host despite pressures, influencing its differentiation, during various plant hosts.

Язык: Английский

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Reference standards for flow cytometric estimation of absolute nuclear DNA content in plants

Cytometry Part A, Год журнала: 2021, Номер 101(9), С. 710 - 724

Опубликована: Авг. 18, 2021

The estimation of nuclear DNA content has been by far the most popular application flow cytometry in plants. Because measures relative fluorescence intensities nuclei stained a fluorochrome, ploidy determination, and absolute units both require comparison to reference standard known content. This implies that quality results obtained depends on selection use. Internal standardization, when an unknown sample are isolated, stained, measured simultaneously, is mandatory for precise measurements. As peaks representing G

Язык: Английский

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Small genome size and variation in ploidy levels support the naturalization of vascular plants but constrain their invasive spread

New Phytologist, Год журнала: 2023, Номер 239(6), С. 2389 - 2403

Опубликована: Июль 12, 2023

Karyological characteristics are among the traits underpinning invasion success of vascular plants. Using 11 049 species, we tested effects genome size and ploidy levels on plant naturalization (species forming self-sustaining populations where they not native) (naturalized species spreading rapidly having environmental impact). The probability that a naturalized anywhere in world decreased with increasing monoploid (DNA content single chromosome set). Naturalized or invasive intermediate genomes were reported from many regions, but those either small large occurred fewer regions. By contrast, holoploid sizes unreplicated gametic nucleus) constrained favoured invasion. We suggest is an advantage during naturalization, being linked to favouring adaptation local conditions, for spread, associated genome, impact polyploidy may act, facilitate long-distance dispersal competition other species.

Язык: Английский

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Evolution of genome size and GC content in the tribe Carduinae (Asteraceae)

Preslia, Год журнала: 2023, Номер 95(1), С. 185 - 213

Опубликована: Янв. 1, 2023

Genome size and GC content are basic species-specific attributes often delimiting genera or higher taxa, which enable the identification of polyploidy, hybridization other modes genome karyotype evolution. The evolution these genomic traits can occur as a result selective pressure environment. Here, we reconstruct in subtribe Carduinae (Asteraceae) context changes chromosome numbers. Using flow cytometry, were estimated for 119 taxa mapped onto phylogenetic tree constructed using sequences from seven genetic markers. In addition, data compared with length stomatal guard cells achene (length, weight) to evaluate extent characters could evolve adaptively this subtribe. We found strong signals analysed phenotypic traits, delimited most clades agreement reconstructed phylogeny. Monoploid was positively correlated cell length. Cirsium, whose species focus majority analyses, large-genomed subgen. Lophiolepis had smaller cells, might be related it occurring more xeric habitats Cirsium. contrast, achenes larger, possibly response summer drought, whereas weight independent across Thus, under weak environmental control, at least that mediated by achenes. Achene content, have evolved drought. Carduus North American Cirsium there is an increase average reduction monoploid number, suggesting descending dysploidy associated chromosomal fusion. Polyploidy relatively rare confirmed only five studied, including vulgare, invasive likely originated via distant (intersubgeneric) hybridization, suggested its intermediate features combined conflict between morphology position. Phylogenetic reconstruction, differences parameters, well sizes support separation monophyletic remainder results also indicate early diverging italicum separated rest Lophiolepis+Picnomon+Notobasis clade.

Язык: Английский

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The Use of Flow Cytometry for Estimating Genome Sizes and DNA Ploidy Levels in Plants

Methods in molecular biology, Год журнала: 2023, Номер unknown, С. 25 - 64

Опубликована: Янв. 1, 2023

Язык: Английский

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New insights into interspecies relationships, chromosomal evolution, and hybrid identification in the Lycoris Herb.

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 21, 2025

Frequent interspecific hybridization, unclear genetic backgrounds, and ambiguous evolutionary relationships within the genus Lycoris pose significant challenges to identification classification of hybrids, thereby impacting application development Lycoris. This study utilizes karyotype structure, genome size, fluorescent in situ hybridization (FISH) technology explore chromosomal evolution hybrid employing three approaches at cytogenetic level. The findings indicate that species with a smaller basic chromosome number exhibit less asymmetry than those larger number, suggesting different numbers may have followed pathways. aurea has more symmetrical karyotype, which be plesiomorphic state, reflecting an transition from symmetry chromosomes. Systematic clustering 18 is consistent classification, primarily dividing into two groups: M + T A type as one group, another group. average nuclear size (C-value) 22.99 Gb, smallest being L. wulingensis (17.10 Gb) largest squamigera (33.06 Gb). Chromosome length positively correlated C-value, haploid (Cx-value) decreases increase (x). FISH technique can quickly identify authenticate artificial thus inferring parentage natural hybrids. reveals background species, identifies authenticity infers possible offering technical insights for identification, genomic projects

Язык: Английский

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Development of tetraploids in tissue culture: modern techniques and biotechnological innovations

Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2025, Номер 160(2)

Опубликована: Фев. 1, 2025

Язык: Английский

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A multidisciplinary and integrative review of the structural genome and epigenome of Capsicum L. species

Planta, Год журнала: 2025, Номер 261(4)

Опубликована: Март 9, 2025

Язык: Английский

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Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii

New Phytologist, Год журнала: 2025, Номер unknown

Опубликована: Март 21, 2025

Whole-genome duplications, widely observed in plant lineages, have significant evolutionary and ecological impacts. Yet, our current understanding of the direct implications ploidy shifts on short- long-term evolution remains fragmentary, necessitating further investigations across multiple levels. Chlamydomonas reinhardtii is a valuable model organism with profound potential to study impact increase longer term laboratory environment. This partly due ability level. We developed strategy engineer C. using noninterfering, antibiotic, selectable markers. approach allows us induce higher levels applicable field isolates, which expands beyond specific auxotroph strains broadens genetic diversity parental haploid that can be crossed. implement flow cytometry for precise measurement genome size different ploidy. demonstrate creation diploids, triploids, tetraploids by engineering North American broadening application synthetic biology principles reinhardtii. However, newly formed triploids show signs rapid aneuploidization. Our greatly facilitates polyploidy, both fundamental applied settings.

Язык: Английский

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