The over-activated peritoneal immune environment in endometriosis is characterised by a lack of PD-1 inhibition. DOI Open Access

Anna Tresso,

Niharika Thota,

Chloé James

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 2, 2024

Abstract Background Endometriosis is characterised by chronic inflammation in the peritoneal cavity causing acute and pelvic pain, largely explained dysregulation immune environment within fluid. The activation status of cells unclear. In addition, a comparison with systemic system desirable to explore avenues diagnosis treatment endometriosis-related pain. Objective(s) To investigate endometriosis fluid blood full-spectrum flow cytometry focus on inhibition cells. Study Design This was an observational study patients undergoing laparoscopy for or unrelated conditions; PF collected from n=5 n=4 controls, n=3 controls. Data were analysed statistical significance using ANOVA, Kruskal-Wallis Mann-Whitney U test, p-value below 0.05 considered significant. Results We observed prevailing myeloid as opposed lymphoid blood. main differences between control samples, however, found smaller compartments, i.e., populations PD-1 levels samples significantly lower than controls (p<0.05). Conclusion(s) checkpoint could be new angle treating pain women suffering this intractable condition. Tweetable Lack plays role inflammation. AJOG at glance inflammatoru needs investigating it iis causative predominant symptom. lack expression compared explain persistent open treatment.

Язык: Английский

Full spectrum flow cytometry and mass cytometry: A 32‐marker panel comparison DOI Creative Commons

María C. Jaimes,

Michael D. Leipold,

Geoffrey Kraker

и другие.

Cytometry Part A, Год журнала: 2022, Номер 101(11), С. 942 - 959

Опубликована: Май 20, 2022

High-dimensional single-cell data has become an important tool in unraveling the complexity of immune system and its involvement homeostasis a large array pathologies. As technological tools are developed, researchers adopting them to answer increasingly complex biological questions. Up until recently, mass cytometry (MC) been main technology employed cytometric assays requiring more than 29 markers. Recently, however, with introduction full spectrum flow (FSFC), it possible break fluorescence barrier go beyond fluorescent parameters. In this study, collaboration Stanford Human Immune Monitoring Center (HIMC), we compared five patient samples using established panel developed by HIMC their MC platform. Using split same antibody panel, were able demonstrate highly comparable results between two technologies multiple analysis approaches. We report here direct comparison platforms (MC FSFC) 32-marker monitoring that can identify all previously described anticipated subpopulations defined panel.

Язык: Английский

Процитировано

31
Proteomics to study cancer immunity and improve treatment DOI Creative Commons
Giulia Franciosa, Anders H. Kverneland, Agnete W.P. Jensen

и другие.

Seminars in Immunopathology, Год журнала: 2023, Номер 45(2), С. 241 - 251

Опубликована: Янв. 4, 2023

Abstract Cancer survival and progression depend on the ability of tumor cells to avoid immune recognition. Advances in understanding cancer immunity escape mechanisms enabled development immunotherapeutic approaches. In patients with otherwise incurable metastatic cancers, immunotherapy resulted unprecedented response rates potential for durable complete responses. However, primary acquired resistance limit efficacy immunotherapy. Further therapeutic advances require a deeper interplay between tumors. Most high-throughput studies within past decade focused an omics characterization at DNA RNA level. proteins are molecular effectors genomic information; therefore, study provides cellular functions. Recent mass spectrometry (MS)-based proteomics system-wide scale may allow translational clinical discoveries by enabling analysis understudied post-translational modifications, subcellular protein localization, cell signaling, protein–protein interactions. this review, we discuss contribution MS-based preclinical research findings context immunotherapies.

Язык: Английский

Процитировано

14
Advanced immunophenotyping: A powerful tool for immune profiling, drug screening, and a personalized treatment approach DOI Creative Commons
Teresa Preglej,

Marie Brinkmann,

G Steiner

и другие.

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Март 24, 2023

Various autoimmune diseases are characterized by distinct cell subset distributions and activation profiles of peripheral blood mononuclear cells (PBMCs). PBMCs can therefore serve as an ideal biomarker material, which is easily accessible allows for screening multiple types. A detailed understanding the immune landscape critical diagnosis patients with diseases, well a personalized treatment approach. In our study, we investigate potential multi-parameter spectral flow cytometry identification suffering from its power evaluation tool in vitro drug approaches (advanced immunophenotyping). We designed combination two 22-color immunophenotyping panels profiling distribution activation. Downstream bioinformatics analyses included percentages individual populations median fluorescent intensity defined markers were then visualized heatmaps dimensionality reduction approaches. testing epigenetic immunomodulatory drugs revealed altered status upon treatment, supports use high-throughput tool. Advanced might support exploration novel therapeutic contribute to future beyond.

Язык: Английский

Процитировано

13
Performance of spectral flow cytometry and mass cytometry for the study of innate myeloid cell populations DOI Creative Commons

Kyra van der Pan,

Indu Khatri, Anniek L. de Jager

и другие.

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Май 19, 2023

Introduction Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well diagnostic patient care. Due to their immunophenotypic heterogeneity biological plasticity, analysis IMC populations typically requires large panels markers. Currently, two cytometry-based techniques allow for the simultaneous detection ≥40 markers: spectral flow cytometry (SFC) mass (MC). However, little known about comparability SFC MC studying populations. Methods We evaluated performance panels, which contained 21 common markers, identification subsetting blood Based on unsupervised clustering analysis, we systematically identified 24 leukocyte populations, including subsets, regardless technique. Results Overall, comparable results were observed between technologies regarding relative distribution these cell staining resolution individual markers (Pearson’s ρ=0.99 0.55, respectively). minor differences intra-measurement variability (median coefficient variation 42.5% vs. 68.0% MC, respectively; p&lt;0.0001) reproducibility, most likely due significantly longer acquisition times 16 min 159 min) lower recovery rates 53.1% 26.8%) associated with MC. Discussion Altogether, our show a good correlation identification, enumeration characterization blood, based (&gt;20) antibody reagents.

Язык: Английский

Процитировано

12
Exploring the role of T cells in Alzheimer's and other neurodegenerative diseases: Emerging therapeutic insights from the T Cells in the Brain symposium DOI Creative Commons
Wassim Elyaman, Lawrence J. Stern, Ning Jiang

и другие.

Alzheimer s & Dementia, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

Abstract This proceedings article summarizes the inaugural “T Cells in Brain” symposium held at Columbia University. Experts gathered to explore role of T cells neurodegenerative diseases. Key topics included characterization antigen‐specific immune responses, cell receptor (TCR) repertoire, microbial etiology Alzheimer's disease (AD), and microglia–T crosstalk, with a focus on how affect neuroinflammation AD biomarkers like amyloid beta tau. The also examined immunotherapies for AD, including Valacyclovir Treatment Disease (VALAD) trial, two clinical trials leveraging regulatory approaches multiple sclerosis amyotrophic lateral therapy. Additionally, single‐cell RNA/TCR sequencing other provided insights into dynamics highlights key findings from outlines future research directions further understand neurodegeneration, offering innovative therapeutic Highlights Researchers discuss study brain disorders. New technologies allow high‐throughput screening cells. Microbial infections can precede several serious chronic neurological Central peripheral responses shape pathology. Immunotherapy induce neuroinflammatory

Язык: Английский

Процитировано

0
CytoPheno: Automated descriptive cell type naming in flow and mass cytometry DOI Creative Commons

Amanda R. Tursi,

Celine S. Lages,

Kenneth Quayle

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 14, 2025

Advances in cytometry have led to increases the number of cellular markers that are routinely measured. The resulting complexity data has prompted a shift from manual automated analysis methods. Currently, numerous unsupervised methods available cluster cells based on marker expression values. However, phenotyping clusters is typically not part process. Manually identifying both definitions (e.g. CD4 + , CCR7 CD45RA CD19 - ) and descriptive cell type names naïve T cells) values can be time-consuming, subjective, error-prone. In this work we propose an algorithm addresses these problems through creation tool, CytoPheno, assigns unidentified clusters. First, post-clustered undergoes per-marker calculations assign as positive or negative. Next, undergo standardization process match Protein Ontology identifier terms. Finally, descriptions matched within Cell Ontology. Each tool was tested with benchmark demonstrate performance. Additionally, encompassed graphical user interface (R Shiny) increase accessibility interpretability. Overall, CytoPheno aid researchers timely unbiased data.

Язык: Английский

Процитировано

0
Single Cell‐Inductively Coupled Plasma‐Time‐of‐Flight Mass Spectrometry for the Multiplexed Determination of Proteins DOI
Paula Menero‐Valdés, Beatriz Fernández, Rosario Pereiro

и другие.

Encyclopedia of Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 1 - 22

Опубликована: Март 27, 2025

Abstract The analysis of proteins in single cells has gained importance the past years because its application biomedical field for study disease pathogenesis, search biomarkers, identification drug targets, and action efficacy, among others. In particular, use sc‐ICP‐TOFMS technique combined with metal‐tagged antibodies allows multiplexed determination target proteins. Moreover, after appropriate labeling, this can provide also information regarding cell size, viability, cycle stage. Here, an overview working principle several considerations which must be taken into account, to obtain accurate reproducible data are presented. effects choice sample introduction system, metal tag obtention metal‐labeled immunoprobes, dwell preparation samples discussed chapter.

Язык: Английский

Процитировано

0
The contributions of T cell-mediated immunity to protection from vaccine-preventable diseases: A primer DOI Creative Commons
Janna R. Shapiro,

Mario Corrado,

Julie Perry

и другие.

Human Vaccines & Immunotherapeutics, Год журнала: 2024, Номер 20(1)

Опубликована: Авг. 29, 2024

In the face of ever-present burden emerging and reemerging infectious diseases, there is a growing need to comprehensively assess individual- population-level immunity vaccine-preventable diseases (VPDs). Many these efforts, however, focus exclusively on antibody-mediated immunity, ignoring role T cells. Aimed at clinicians, public health practioners, others who play central roles in human vaccine research but do not have formal training immunology, we review how vaccines against elicit cell responses, what types responses are measured. We then use examples demonstrate six ways that cells contribute protection from VPD, including directly mediating protection, enabling antibody reducing disease severity, increasing cross-reactivity, improving durability, protecting special populations. conclude with discussion challenges solutions more widespread consideration clinical vaccinology.

Язык: Английский

Процитировано

3
tidytof: a user-friendly framework for scalable and reproducible high-dimensional cytometry data analysis DOI Creative Commons
Timothy Keyes, Abhishek Koladiya,

Yu‐Chen Lo

и другие.

Bioinformatics Advances, Год журнала: 2023, Номер 3(1)

Опубликована: Янв. 1, 2023

While many algorithms for analyzing high-dimensional cytometry data have now been developed, the software implementations of these remain highly customized-this means that exploring a dataset requires users to learn unique, often poorly interoperable package syntaxes each step processing. To solve this problem, we developed {tidytof}, an open-source R using increasingly popular 'tidy data' interface.{tidytof} is available at https://github.com/keyes-timothy/tidytof and released under MIT license. It supported on Linux, MS Windows MacOS. Additional documentation website (https://keyes-timothy.github.io/tidytof/).Supplementary are Bioinformatics Advances online.

Язык: Английский

Процитировано

8
Spectral flow cytometry: Fundamentals and future impact DOI
J. Paul Robinson, Bartek Rajwa

Methods in cell biology, Год журнала: 2024, Номер unknown, С. 311 - 332

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

2