Cell Communication and Signaling,
Год журнала:
2023,
Номер
21(1)
Опубликована: Май 11, 2023
Cardiovascular
diseases,
such
as
myocardial
infarction
(MI),
are
a
leading
cause
of
death
worldwide.
Acute
MI
(AMI)
inflicts
massive
injury
to
the
coronary
microcirculation,
causing
large-scale
cardiomyocyte
due
ischemia
and
hypoxia.
Inflammatory
cells
monocytes
macrophages
migrate
damaged
area
clear
away
dead
post-MI.
Macrophages
pleiotropic
innate
immune
system,
which
play
an
essential
role
in
initial
inflammatory
response
that
occurs
following
MI,
inducing
subsequent
damage
facilitating
recovery.
Besides
their
recognized
within
response,
participate
crosstalk
with
other
(including
cardiomyocytes,
fibroblasts,
cells,
vascular
endothelial
cells)
coordinate
post-MI
processes
cardiac
tissue.
Macrophage-secreted
exosomes
have
recently
attracted
increasing
attention,
has
led
more
elaborate
understanding
macrophage
function.
Currently,
functional
roles
microenvironment
infarcted
heart,
particularly
regard
interaction
surrounding
remain
unclear.
Understanding
specific
mechanisms
mediate
this
is
treating
MI.
In
review,
we
discuss
origin
macrophages,
changes
distribution
post-MI,
phenotypic
plasticity,
well
signaling
pathways
involved,
focus
on
heart.
Thus,
provide
new
perspective
treatment
Further
in-depth
research
required
elucidate
underlying
between
tissue
for
identification
potential
therapeutic
targets.
Video
Abstract.
Bioanalysis,
Год журнала:
2021,
Номер
13(3), С. 181 - 198
Опубликована: Фев. 1, 2021
Flow
cytometry
(FCM)
is
a
sophisticated
technique
that
works
on
the
principle
of
light
scattering
and
fluorescence
emission
by
specific
fluorescent
probe-labeled
cells
as
they
pass
through
laser
beam.
It
offers
several
unique
advantages
it
allows
fast,
relatively
quantitative,
multiparametric
analysis
cell
populations
at
single
level.
In
addition,
also
enables
physical
sorting
to
separate
subpopulations
based
different
parameters.
this
constantly
evolving
field,
innovative
technologies
such
imaging
FCM,
mass
Raman
FCM
are
being
developed
in
order
address
limitations
traditional
FCM.
This
review
explains
general
principles,
main
applications
recent
advances
field
Cells,
Год журнала:
2023,
Номер
12(14), С. 1875 - 1875
Опубликована: Июль 17, 2023
Unmasking
the
subtleties
of
immune
system
requires
both
a
comprehensive
knowledge
base
and
ability
to
interrogate
that
with
intimate
sensitivity.
That
task,
considerable
extent,
has
been
handled
by
an
iterative
expansion
in
flow
cytometry
methods,
technological
capability
also
accompanying
advances
informatics.
As
field
fluorescence-based
cytomics
matured,
it
reached
barrier
at
around
30
parameter
analyses,
which
stalled
until
spectral
created
fundamental
transformation
will
likely
lead
potential
100
simultaneous
analyses
within
few
years.
The
advance
informatics
now
become
watershed
moment
for
as
competes
mature
systematic
approaches
such
genomics
proteomics,
allowing
take
seat
multi-omics
table.
In
addition,
recent
try
combine
speed
systems
other
detection
addition
fluorescence
alone,
make
flow-based
instruments
even
more
indispensable
any
biological
laboratory.
This
paper
outlines
current
cell
analysis
discusses
traditional
microfluidic
sorting
well
next-generation
instruments,
provides
early
look
future
opportunities
are
arise.
Science,
Год журнала:
2024,
Номер
383(6681), С. 413 - 421
Опубликована: Янв. 25, 2024
Age-associated
B
cells
(ABCs)
accumulate
during
infection,
aging,
and
autoimmunity,
contributing
to
lupus
pathogenesis.
In
this
study,
we
screened
for
transcription
factors
driving
ABC
formation
found
that
zinc
finger
E-box
binding
homeobox
2
(ZEB2)
is
required
human
mouse
differentiation
in
vitro.
ABCs
are
reduced
ZEB2
haploinsufficient
individuals
mice
lacking
Zeb2
cells.
with
toll-like
receptor
7
(TLR7)–driven
lupus,
essential
autoimmune
pathology.
binds
+20-kb
myocyte
enhancer
factor
2b
(
Mef2b
)’s
intronic
enhancer,
repressing
MEF2B-mediated
germinal
center
cell
promoting
formation.
also
targets
genes
important
specification
function,
including
Itgax
.
ZEB2-driven
requires
JAK-STAT
(Janus
kinase-signal
transducer
activator
of
transcription),
treatment
JAK1/3
inhibitor
reduces
accumulation
patients.
Thus,
emerges
as
a
driver
autoimmunity.
Cell Communication and Signaling,
Год журнала:
2023,
Номер
21(1)
Опубликована: Май 11, 2023
Cardiovascular
diseases,
such
as
myocardial
infarction
(MI),
are
a
leading
cause
of
death
worldwide.
Acute
MI
(AMI)
inflicts
massive
injury
to
the
coronary
microcirculation,
causing
large-scale
cardiomyocyte
due
ischemia
and
hypoxia.
Inflammatory
cells
monocytes
macrophages
migrate
damaged
area
clear
away
dead
post-MI.
Macrophages
pleiotropic
innate
immune
system,
which
play
an
essential
role
in
initial
inflammatory
response
that
occurs
following
MI,
inducing
subsequent
damage
facilitating
recovery.
Besides
their
recognized
within
response,
participate
crosstalk
with
other
(including
cardiomyocytes,
fibroblasts,
cells,
vascular
endothelial
cells)
coordinate
post-MI
processes
cardiac
tissue.
Macrophage-secreted
exosomes
have
recently
attracted
increasing
attention,
has
led
more
elaborate
understanding
macrophage
function.
Currently,
functional
roles
microenvironment
infarcted
heart,
particularly
regard
interaction
surrounding
remain
unclear.
Understanding
specific
mechanisms
mediate
this
is
treating
MI.
In
review,
we
discuss
origin
macrophages,
changes
distribution
post-MI,
phenotypic
plasticity,
well
signaling
pathways
involved,
focus
on
heart.
Thus,
provide
new
perspective
treatment
Further
in-depth
research
required
elucidate
underlying
between
tissue
for
identification
potential
therapeutic
targets.
Video
Abstract.
