Cells,
Journal Year:
2023,
Volume and Issue:
12(14), P. 1875 - 1875
Published: July 17, 2023
Unmasking
the
subtleties
of
immune
system
requires
both
a
comprehensive
knowledge
base
and
ability
to
interrogate
that
with
intimate
sensitivity.
That
task,
considerable
extent,
has
been
handled
by
an
iterative
expansion
in
flow
cytometry
methods,
technological
capability
also
accompanying
advances
informatics.
As
field
fluorescence-based
cytomics
matured,
it
reached
barrier
at
around
30
parameter
analyses,
which
stalled
until
spectral
created
fundamental
transformation
will
likely
lead
potential
100
simultaneous
analyses
within
few
years.
The
advance
informatics
now
become
watershed
moment
for
as
competes
mature
systematic
approaches
such
genomics
proteomics,
allowing
take
seat
multi-omics
table.
In
addition,
recent
try
combine
speed
systems
other
detection
addition
fluorescence
alone,
make
flow-based
instruments
even
more
indispensable
any
biological
laboratory.
This
paper
outlines
current
cell
analysis
discusses
traditional
microfluidic
sorting
well
next-generation
instruments,
provides
early
look
future
opportunities
are
arise.
Immunity,
Journal Year:
2021,
Volume and Issue:
54(4), P. 702 - 720.e17
Published: March 30, 2021
Murine
regulatory
T
(Treg)
cells
in
tissues
promote
tissue
homeostasis
and
regeneration.
We
sought
to
identify
features
that
characterize
human
Treg
with
these
functions
healthy
tissues.
Single-cell
chromatin
accessibility
profiles
of
murine
defined
a
conserved,
microbiota-independent
tissue-repair
signature
prevailing
footprint
the
transcription
factor
BATF.
This
signature,
combined
gene
expression
profiling
TCR
fate
mapping,
identified
population
tissue-like
peripheral
blood
expressed
BATF,
chemokine
receptor
CCR8
HLA-DR.
Human
BATF+CCR8+
from
normal
skin
adipose
shared
nonlymphoid
follicular
helper-like
(Tfh-like)
cells,
induction
Tfh-like
differentiation
program
naive
partially
recapitulated
regenerative
characteristics,
including
wound
healing
potential.
share
tumor-resident
highlighting
importance
understanding
context-specific
cells.
Bioanalysis,
Journal Year:
2021,
Volume and Issue:
13(3), P. 181 - 198
Published: Feb. 1, 2021
Flow
cytometry
(FCM)
is
a
sophisticated
technique
that
works
on
the
principle
of
light
scattering
and
fluorescence
emission
by
specific
fluorescent
probe-labeled
cells
as
they
pass
through
laser
beam.
It
offers
several
unique
advantages
it
allows
fast,
relatively
quantitative,
multiparametric
analysis
cell
populations
at
single
level.
In
addition,
also
enables
physical
sorting
to
separate
subpopulations
based
different
parameters.
this
constantly
evolving
field,
innovative
technologies
such
imaging
FCM,
mass
Raman
FCM
are
being
developed
in
order
address
limitations
traditional
FCM.
This
review
explains
general
principles,
main
applications
recent
advances
field
Journal of Extracellular Vesicles,
Journal Year:
2023,
Volume and Issue:
12(2)
Published: Feb. 1, 2023
Flow
cytometry
(FCM)
offers
a
multiparametric
technology
capable
of
characterizing
single
extracellular
vesicles
(EVs).
However,
most
flow
cytometers
are
designed
to
detect
cells,
which
larger
than
EVs.
Whereas
cells
exceed
the
background
noise,
signals
originating
from
EVs
partly
overlap
with
thereby
making
more
difficult
cells.
This
technical
mismatch
together
complexity
EV-containing
fluids
causes
limitations
and
challenges
conducting,
interpreting
reproducing
EV
FCM
experiments.
To
address
overcome
these
challenges,
researchers
International
Society
for
Extracellular
Vesicles
(ISEV),
Advancement
Cytometry
(ISAC),
on
Thrombosis
Haemostasis
(ISTH)
joined
forces
initiated
working
group.
improve
interpretation,
reporting,
reproducibility
future
data,
group
published
an
ISEV
position
manuscript
outlining
framework
minimum
information
that
should
be
reported
about
experiment
(MIFlowCyt-EV).
contains
limited
information.
Therefore,
goal
this
compendium
is
provide
necessary
design
conduct
reproducible
EVs,
interaction
between
light
hardware,
experimental
preanalytical
procedures,
sample
preparation,
assay
controls,
instrument
data
acquisition
calibration,
characterization,
reporting.
Although
focuses
many
concepts
explanations
could
also
applied
detection
other
particles
within
size
range,
such
as
bacteria,
lipoprotein
particles,
milk
fat
globules,
viruses.
Cells,
Journal Year:
2023,
Volume and Issue:
12(14), P. 1875 - 1875
Published: July 17, 2023
Unmasking
the
subtleties
of
immune
system
requires
both
a
comprehensive
knowledge
base
and
ability
to
interrogate
that
with
intimate
sensitivity.
That
task,
considerable
extent,
has
been
handled
by
an
iterative
expansion
in
flow
cytometry
methods,
technological
capability
also
accompanying
advances
informatics.
As
field
fluorescence-based
cytomics
matured,
it
reached
barrier
at
around
30
parameter
analyses,
which
stalled
until
spectral
created
fundamental
transformation
will
likely
lead
potential
100
simultaneous
analyses
within
few
years.
The
advance
informatics
now
become
watershed
moment
for
as
competes
mature
systematic
approaches
such
genomics
proteomics,
allowing
take
seat
multi-omics
table.
In
addition,
recent
try
combine
speed
systems
other
detection
addition
fluorescence
alone,
make
flow-based
instruments
even
more
indispensable
any
biological
laboratory.
This
paper
outlines
current
cell
analysis
discusses
traditional
microfluidic
sorting
well
next-generation
instruments,
provides
early
look
future
opportunities
are
arise.
