Role of Copper and Zinc Ions in the Hydrolytic Degradation of Neurodegeneration-Related Peptides

Molecules, Год журнала: 2025, Номер 30(2), С. 363 - 363

Опубликована: Янв. 17, 2025

Spontaneous cleavage reactions normally occur in vivo on amino acid peptide backbones, leading to fragmentation products that can have different physiological roles and toxicity, particularly when the substrate of hydrolytic processes are neuronal peptides proteins highly related neurodegeneration. We report a study performed with HPLC-MS technique at temperatures (4 °C 37 °C) fragments (amyloid-β, tau, α-synuclein) conditions presence Cu2+ Zn2+ ions, two metal ions found millimolar concentrations amyloid plaques. The coordination these significantly protects their backbones toward degradation, preserving entire sequences over weeks solution, while free same buffer fully fragmented after or even shorter incubation period. Our data show is not only ruled by chemical sensitivity acids, but conformation changes induced influence reactions. enhanced stability provided increase local levels amyloidogenic species capable seeding fibril growth, resulting aberrant protein depositions deficits activity.

Язык: Английский

Ion-induced conformational dynamics of collagen fibrils: Molecular mechanism

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 316, С. 144728 - 144728

Опубликована: Май 27, 2025

Язык: Английский