Role of Copper and Zinc Ions in the Hydrolytic Degradation of Neurodegeneration-Related Peptides DOI Creative Commons
Valentina Pirota, Enrico Monzani, Simone Dell’Acqua

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(2), P. 363 - 363

Published: Jan. 17, 2025

Spontaneous cleavage reactions normally occur in vivo on amino acid peptide backbones, leading to fragmentation products that can have different physiological roles and toxicity, particularly when the substrate of hydrolytic processes are neuronal peptides proteins highly related neurodegeneration. We report a study performed with HPLC-MS technique at temperatures (4 °C 37 °C) fragments (amyloid-β, tau, α-synuclein) conditions presence Cu2+ Zn2+ ions, two metal ions found millimolar concentrations amyloid plaques. The coordination these significantly protects their backbones toward degradation, preserving entire sequences over weeks solution, while free same buffer fully fragmented after or even shorter incubation period. Our data show is not only ruled by chemical sensitivity acids, but conformation changes induced influence reactions. enhanced stability provided increase local levels amyloidogenic species capable seeding fibril growth, resulting aberrant protein depositions deficits activity.

Language: Английский

Role of Copper and Zinc Ions in the Hydrolytic Degradation of Neurodegeneration-Related Peptides DOI Creative Commons
Valentina Pirota, Enrico Monzani, Simone Dell’Acqua

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(2), P. 363 - 363

Published: Jan. 17, 2025

Spontaneous cleavage reactions normally occur in vivo on amino acid peptide backbones, leading to fragmentation products that can have different physiological roles and toxicity, particularly when the substrate of hydrolytic processes are neuronal peptides proteins highly related neurodegeneration. We report a study performed with HPLC-MS technique at temperatures (4 °C 37 °C) fragments (amyloid-β, tau, α-synuclein) conditions presence Cu2+ Zn2+ ions, two metal ions found millimolar concentrations amyloid plaques. The coordination these significantly protects their backbones toward degradation, preserving entire sequences over weeks solution, while free same buffer fully fragmented after or even shorter incubation period. Our data show is not only ruled by chemical sensitivity acids, but conformation changes induced influence reactions. enhanced stability provided increase local levels amyloidogenic species capable seeding fibril growth, resulting aberrant protein depositions deficits activity.

Language: Английский

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