Analytical Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 10, 2024
come
from
teaching
and
research
institutions
in
France
or
abroad,
public
private
centers.L'archive
ouverte
pluridisciplinaire
HAL,
est
destinée
au
dépôt
et
à
la
diffusion
de
documents
scientifiques
niveau
recherche,
publiés
ou
non,
émanant
des
établissements
d'enseignement
recherche
français
étrangers,
laboratoires
publics
privés.
Getting
the
best
out
of
capillary
electrophoresis
andcapillary
electrophoresis-mass
spectrometry
by
quantifying
sources
peak
broadening
for
proteins
using
polyelectrolyte
multilayer
coated
fused
silica
capillaries
TrAC Trends in Analytical Chemistry,
Год журнала:
2023,
Номер
171, С. 117490 - 117490
Опубликована: Дек. 19, 2023
Proteins
are
efficient
therapeutic
agents
and
attractive
potential
new
biomarkers
due
to
their
physiological
pathological
functions.
Further,
biopharmaceuticals
feature
complex
protein
architectures
that
must
be
assayed
quality
controlled.
Capillary
electrophoresis
(CE)
has
become
one
of
the
most
powerful
tools
for
proteomic
analysis.
CE
is
an
eco-friendlier
alternative
traditional
liquid
chromatography;
however,
its
true
greenness
environmental
impact
not
very
often
confirmed
by
green
metrics.
Here,
we
assessed
bottom-up,
middle-up,
top-down
sample
preparation
analytical
approaches
analysis
proteins
using
AGREEprep
AGREE
metric
systems,
respectively.
The
combined
use
both
can
help
identify
weaknesses
overall
procedure
point
toward
greener
alternatives.
results
showed
trends
in
workflows
should
focused
mainly
on
miniaturization
devices
applying
in-line
on-line
preparation.
Electrophoresis,
Год журнала:
2023,
Номер
44(9-10), С. 767 - 774
Опубликована: Янв. 31, 2023
The
complexity
of
biotherapeutic
products
implies
an
ever-increasing
list
product
quality
attributes
that
need
to
be
monitored
and
characterized.
In
addition,
the
growing
interest
in
implementing
process
analytical
technology
biopharmaceutical
production
has
further
increased
testing
burden,
together
with
for
rapid
can
facilitate
real-time
or
near-real-time
decision-making.
Capillary
electrophoresis
(CE)
made
a
place
analysis
but
is
regarded
as
low-throughput
method,
instrument
dead
time
constituting
more
than
80%
total
analysis.
this
study,
CE
was
utilized
analyse
3
mAb
samples
single-CE
run.
This
approach
resulted
up
77%
reduction
productivity
by
300%,
compared
traditional
single
CE-ultraviolet
runs,
without
compromising
resolution
relative
peak
areas.
Additionally,
good
method
reproducibility
observed.
compatibility
been
demonstrated
protein
A
eluate
cation
exchange
chromatography
fractions.
We,
thus,
propose
sequential
injections
applied
fast
robust
biopharmaceuticals.
Abstract
Electrophoresis
of
a
weakly
charged
dielectric
droplet
with
constant
surface
charge
density
in
chargeless
cylindrical
pore
is
investigated
theoretically
this
study,
focusing
on
the
boundary
confinement
effect
double
layer,
which
turn
determines
ultimate
motion
droplet.
A
patched
pseudo‐spectral
method
based
Chebyshev
polynomial
adopted
to
solve
resulting
governing
fundamental
electrokinetic
equations.
Mobility
reversal,
among
other
interesting
phenomena,
observed
when
narrow
pore.
No
such
observation
was
made
corresponding
rigid
particle.
The
thick
layer
may
even
move
against
prediction
Coulomb
electrostatic
law,
for
instance,
positively
electric
field.
significant
enhancement
motion‐deterring
polarization
due
severe
steric
within
found
be
responsible
seemingly
peculiar
phenomenon.
Moreover,
smaller
droplets
opposite
direction
larger
ones.
results
are
useful
capillary
electrophoresis
involving
particular
and
migration
through
channels
general.
Pharmaceuticals,
Год журнала:
2024,
Номер
17(7), С. 925 - 925
Опубликована: Июль 10, 2024
This
research
is
based
on
three
fundamental
aspects
of
successful
biosimilar
development
in
the
challenging
biopharmaceutical
market.
First,
regulations
eight
selected
countries:
Japan,
South
Korea,
United
States,
Canada,
Brazil,
Argentina,
Australia,
and
Africa,
represent
four
continents.
The
regulatory
countries
studied
are
analyzed,
highlighting
challenges
facing
biosimilars,
including
their
complex
approval
processes
need
for
standardized
guidelines.
There
an
inconsistency
depending
whether
used
a
developed
or
developing
country.
In
observed,
biosimilars
considered
excellent
alternatives
to
patent-protected
biological
products
treatment
chronic
diseases.
second
aspect
addressed,
various
analytical
AI
modeling
methods
(such
as
machine
learning
tools,
reinforcement
learning,
supervised,
unsupervised,
deep
tools)
were
analyzed
observe
patterns
that
lead
prevalence
cancer
model
behaviors
most
prominent
active
compounds
with
spectroscopy.
Finally,
analysis
use
approved
by
FDA
EMA
was
proposed.
Analytical Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 10, 2024
come
from
teaching
and
research
institutions
in
France
or
abroad,
public
private
centers.L'archive
ouverte
pluridisciplinaire
HAL,
est
destinée
au
dépôt
et
à
la
diffusion
de
documents
scientifiques
niveau
recherche,
publiés
ou
non,
émanant
des
établissements
d'enseignement
recherche
français
étrangers,
laboratoires
publics
privés.
Getting
the
best
out
of
capillary
electrophoresis
andcapillary
electrophoresis-mass
spectrometry
by
quantifying
sources
peak
broadening
for
proteins
using
polyelectrolyte
multilayer
coated
fused
silica
capillaries
