Biomacromolecules,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 23, 2025
Enzymes
are
attractive
as
catalysts
due
to
their
specificity
and
biocompatibility;
however,
use
in
industrial
biomedical
applications
is
limited
by
stability.
Here,
we
present
a
facile
approach
for
enzyme
immobilization
within
"all-enzyme"
hydrogels
forming
photochemical
covalent
cross-links
between
the
glucose
oxidase.
We
demonstrate
that
mechanical
properties
of
hydrogel
can
be
tuned
with
concentration
data
suggests
dimeric
nature
oxidase
results
unusual
gel
formation
behavior
which
degree
forced
induced
dimer
dissociation
unfolding.
confirm
quantify
activity
using
Trinder
assay
1D
modeling
show
50%
enzymatic
retained
experimental
shows
Michaelis
constant
KM
upon
formation.
These
observed
effects
may
forces
experienced
individual
nanoscale
enzymes
during
mesoscale
network
have
therefore
demonstrated
cross-linking
readily
employed
produce
functional
all-enzyme
easily
tunable
specific
catalytic
activity.
This
provides
enormous
potential
producing
biocatalytic
materials
properties,
responsive
biological
functionality
high
volumetric
productivity
inform
future
design
devices
enhanced
sensitivity
Food Chemistry Advances,
Год журнала:
2023,
Номер
2, С. 100192 - 100192
Опубликована: Янв. 19, 2023
Pectin
is
a
complex
polysaccharide
of
great
importance
to
food,
pharmaceutical
and
cosmetic
industries.
It
widely
used
in
the
food
industry
as
thickener,
gelling
agent
an
emulsifier
among
others.
also
exhibits
potential
anti-inflammatory
immunomodulatory
effects.
Its
functional
properties
are
determined
by
characteristics
such
neutral
sugar
side
chains,
molecular
weight
degree
methyl-esterification.
Human
beings
unable
digest
pectin
therefore
pectinases
catalyze
degradation
pectic
substances.
This
review
focuses
on
pectin,
its
composition,
application
those
enzymes
involved
degradation,
different
agro-industrial
residues
sources
extraction
methods
future
perspectives.
Processes,
Год журнала:
2022,
Номер
10(3), С. 494 - 494
Опубликована: Март 1, 2022
Enzymes
are
outstanding
(bio)catalysts,
not
solely
on
account
of
their
ability
to
increase
reaction
rates
by
up
several
orders
magnitude
but
also
for
the
high
degree
substrate
specificity,
regiospecificity
and
stereospecificity.
The
use
development
enzymes
as
robust
biocatalysts
is
one
main
challenges
in
biotechnology.
However,
despite
specificities
turnover
enzymes,
there
drawbacks.
At
industrial
level,
these
drawbacks
typically
overcome
resorting
immobilized
enhance
stability.
Immobilization
allows
reuse,
increases
stability,
facilitates
process
control,
eases
product
recovery,
enhances
yield
quality.
This
especially
important
expensive
those
obtained
low
fermentation
with
relatively
activity.
review
provides
an
integrated
perspective
(multi)enzyme
immobilization
that
abridges
a
critical
evaluation
methods
carriers,
biocatalyst
metrics,
impact
key
carrier
features
performance,
trends
towards
miniaturization
detailed
illustrative
examples
representative
biocatalytic
applications
promoting
sustainability.
Engineering in Life Sciences,
Год журнала:
2021,
Номер
22(3-4), С. 165 - 177
Опубликована: Дек. 21, 2021
Abstract
Biocatalysis
is
an
established
chemical
synthesis
technology
that
has
by
no
means
been
restricted
to
research
laboratories.
The
use
of
enzymes
for
organic
evolved
greatly
from
early
development
proof‐of‐concept
–
small
batch
production
industrial
scale.
Different
enzyme
immobilization
strategies
contributed
this
success
story.
Recently,
the
hydrogel
materials
attracting
great
interest.
Within
review,
we
pay
special
attention
recent
developments
in
key
emerging
field
research.
Firstly,
will
briefly
introduce
concepts
both
biocatalysis
and
worlds.
Then,
list
interesting
publications
link
concepts.
Finally,
provide
outlook
comment
on
future
perspectives
further
exploration
hydrogels.
Biosensors and Bioelectronics,
Год журнала:
2023,
Номер
227, С. 115146 - 115146
Опубликована: Фев. 10, 2023
Three-dimensional
(3D)
printed
electrochemical
devices
are
increasingly
used
in
point-of-need
and
point-of-care
testing.
They
show
several
advantages
such
as
simple
fabrication,
low
cost,
fast
response,
excellent
selectivity
sensitivity
small
sample
volumes.
However,
there
only
a
few
examples
of
analytical
combining
3D-printed
electrodes
with
electrochemiluminescence
(ECL)
detection,
an
detection
principle
widely
employed
clinical
chemistry
analysis.
Herein,
portable,
miniaturized
ECL
biosensor
for
glucose
has
been
developed,
based
on
the
luminol/H2O2
system
employing
two-electrode
configuration
carbon
black-doped
polylactic
acid
(PLA)
electrodes.
The
emission
is
obtained
by
means
1.5V
AA
alkaline
battery
detected
using
smartphone
camera,
thus
providing
easy
portability
platform.
was
successfully
applied
sensing
H2O2
and,
upon
coupling
enzyme
oxidase,
detection.
incorporation
luminol
oxidase
agarose
hydrogel
matrix
allowed
to
produce
preloaded
reagents
required
assay,
so
that
analysis
addition.
showed
ability
detect
up
5
mmol
L−1,
limit
60
μmol
L−1.
also
analyse
real
samples
(i.e.,
saline
solutions
artificial
serum
samples)
satisfactory
results,
suggesting
its
suitability
Coupling
other
oxidases
could
further
extend
applicability
this
Green Chemistry,
Год журнала:
2023,
Номер
25(12), С. 4591 - 4624
Опубликована: Янв. 1, 2023
This
review
details
the
potential
of
BDFMs
as
promising
carriers
for
enzymes
design
sustainable
and
robust
biocatalysts
with
improved
activity,
stability,
reusability.
Current Opinion in Biotechnology,
Год журнала:
2022,
Номер
78, С. 102835 - 102835
Опубликована: Ноя. 2, 2022
In
this
review,
we
focus
on
the
holistic
continuous
enzymatic
production
and
put
special
emphasis
process
intensification
by
up-
downstream
processing
in
flow
biocatalysis.
After
a
brief
introduction,
provide
an
overview
of
current
examples
enzyme
immobilization
as
upstream
for
Thereafter,
unit
operations
strategies,
namely
(i)
liquid-liquid
extraction,
(ii)
adsorptive
processing,
(iii)
crystallization
precipitation.
Eventually,
present
our
perspectives
future
trends
research
field.
Advanced Healthcare Materials,
Год журнала:
2022,
Номер
12(1)
Опубликована: Окт. 27, 2022
Novel
biomaterials
for
bio-
and
chemical
sensing
applications
have
gained
considerable
traction
in
the
diagnostic
community
with
rising
trends
of
using
biocompatible
lowly
cytotoxic
material.
Hydrogel-based
electrochemical
sensors
become
a
promising
candidate
their
swellable,
nano-/microporous,
aqueous
3D
structures
capable
immobilizing
catalytic
enzymes,
electroactive
species,
whole
cells,
complex
tissue
models,
while
maintaining
tunable
mechanical
properties
wearable
implantable
applications.
With
advances
highly
controllable
fabrication
processability
these
novel
biomaterials,
possibility
bio-nanocomposite
hydrogel-based
presents
paradigm
shift
development
biocompatible,
"smart,"
sensitive
health
monitoring
point-of-care
devices.
Here,
recent
hydrogels
detection
biomarkers
vitro,
situ,
vivo
are
briefly
reviewed
to
demonstrate
applicability
ideal
conditions,
cellular
environments,
live
animal
respectively,
provide
comprehensive
assessment
whether
ready
translation
biointegration.
Sensors
based
on
conductive
nonconductive
polymers
presented,
highlights
nano-/microstructured
electrodes
that
enhanced
sensitivity
selectivity
matrices.
An
outlook
current
challenges
shall
be
addressed
realization
truly
continuous
real-time
platforms
is
also
presented.
Biosensors,
Год журнала:
2022,
Номер
13(1), С. 44 - 44
Опубликована: Дек. 28, 2022
This
review
provides
a
general
overview
of
different
biosensors,
mostly
concentrating
on
electrochemical
analytical
devices,
while
briefly
explaining
approaches
to
various
kinds
their
construction
and
performance.
A
discussion
how
all
required
components
biosensors
are
brought
together
perform
work
is
offered.
Different
signal-transducing
mechanisms
discussed,
particularly
addressing
the
immobilization
biomolecular
in
vicinity
transducer
interface
functional
integration
with
electronic
devices.
The
concepts
biosensing
processes
rather
than
specific
modern
achievements
area.
