Signal Transduction and Targeted Therapy,
Год журнала:
2023,
Номер
8(1)
Опубликована: Май 24, 2023
Abstract
Current
attempts
in
vaccine
delivery
systems
concentrate
on
replicating
the
natural
dissemination
of
live
pathogens,
but
neglect
that
pathogens
evolve
to
evade
immune
system
rather
than
provoke
it.
In
case
enveloped
RNA
viruses,
it
is
nucleocapsid
protein
(NP,
core
antigen)
and
surface
antigen
delays
NP
exposure
surveillance.
Here,
we
report
a
multi-layered
aluminum
hydroxide-stabilized
emulsion
(MASE)
dictate
sequence
antigens.
this
manner,
receptor-binding
domain
(RBD,
spike
was
trapped
inside
nanocavity,
while
absorbed
outside
droplets,
enabling
burst
release
before
RBD.
Compared
with
packaging
strategy,
inside-out
strategy
induced
potent
type
I
interferon-mediated
innate
responses
triggered
an
immune-potentiated
environment
advance,
which
subsequently
boosted
CD40
+
DC
activations
engagement
lymph
nodes.
both
H1N1
influenza
SARS-CoV-2
vaccines,
rMASE
significantly
increased
antigen-specific
antibody
secretion,
memory
T
cell
engagement,
Th1-biased
response,
diminished
viral
loads
after
lethal
challenge.
By
simply
reversing
antigen,
may
offer
major
implications
for
enhanced
vaccinations
against
virus.
ACS Materials Au,
Год журнала:
2023,
Номер
3(6), С. 600 - 619
Опубликована: Авг. 21, 2023
Over
the
past
decade,
therapeutic
potential
of
nanomaterials
as
novel
drug
delivery
systems
complementing
conventional
pharmacology
has
been
widely
acknowledged.
Among
these
nanomaterials,
lipid-based
nanoparticles
(LNPs)
have
shown
remarkable
pharmacological
performance
and
promising
outcomes,
thus
gaining
substantial
interest
in
preclinical
clinical
research.
In
this
review,
we
introduce
main
types
LNPs
used
formulations
such
liposomes,
nanoemulsions,
solid
lipid
nanoparticles,
nanostructured
carriers,
polymer
hybrid
focusing
on
their
physicochemical
properties
potential.
We
discuss
computational
studies
modeling
techniques
to
enhance
understanding
how
interact
with
cargo
predict
effectiveness
interactions
applications.
also
analyze
benefits
drawbacks
various
LNP
production
nanoprecipitation,
emulsification,
evaporation,
thin
film
hydration,
microfluidic-based
methods,
an
impingement
jet
mixer.
Additionally,
major
challenges
associated
industrial
development,
including
stability
sterilization,
storage,
regulatory
compliance,
reproducibility,
quality
control.
Overcoming
facilitating
compliance
represent
key
steps
toward
LNP's
successful
commercialization
translation
into
settings.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Март 25, 2023
Abstract
Nanomedicine
holds
great
promise
to
enhance
cancer
therapy.
However,
low
active
pharmaceutical
ingredient
(API)
loading
content,
unpredictable
drug
release,
and
potential
toxicity
from
excipients
limit
their
translational
capability.
We
herein
report
a
full-API
nanodrug
composed
of
FDA-approved
5-aminolevulinic
acid
(ALA),
human
essential
element
Fe
3+
,
natural
bioactive
compound
curcumin
with
an
ideal
API
content
pH-responsive
release
profile
for
continuous
spatiotemporal
therapy
achieved
by
multi-step
tandem
endogenous
biosynthesis.
First,
ALA
enzymatically
converts
into
photosensitizer
protoporphyrin
IX
(PpIX).
Afterward,
multiple
downstream
products
including
carbon
monoxide
(CO),
2+
biliverdin
(BV),
bilirubin
(BR)
are
individually
biosynthesized
through
the
PpIX-heme-CO/Fe
/BV-BR
metabolic
pathway,
further
cooperating
released
curcumin,
ultimately
eliciting
mitochondria
damage,
membrane
disruption,
intracytoplasmic
injury.
This
work
not
only
provides
paradigm
exploiting
diversified
metabolites
tumor
suppression,
but
also
presents
safe
efficient
nanodrug,
facilitating
practical
translation
nanodrugs.
Chemical Reviews,
Год журнала:
2024,
Номер
124(20), С. 11242 - 11347
Опубликована: Окт. 9, 2024
Biopsy,
including
tissue
and
liquid
biopsy,
offers
comprehensive
real-time
physiological
pathological
information
for
disease
detection,
diagnosis,
monitoring.
Fluorescent
probes
are
frequently
selected
to
obtain
adequate
on
processes
in
a
rapid
minimally
invasive
manner
based
their
advantages
biopsy.
However,
conventional
fluorescent
have
been
found
show
aggregation-caused
quenching
(ACQ)
properties,
impeding
greater
progresses
this
area.
Since
the
discovery
of
aggregation-induced
emission
luminogen
(AIEgen)
promoted
advancements
molecular
bionanomaterials
owing
unique
high
quantum
yield
(QY)
signal-to-noise
ratio
(SNR),
Abstract
Coronavirus
disease
2019
(COVID‐19)
continually
poses
a
significant
threat
to
the
human
race,
and
prophylactic
vaccination
is
most
potent
approach
end
this
pandemic.
Nanotechnology
widely
adopted
during
COVID‐19
vaccine
development,
engineering
of
nanostructured
materials
such
as
nanoparticles
has
opened
new
possibilities
in
innovative
development
by
improving
design
accelerating
process.
This
review
aims
comprehensively
understand
current
situation
prospects
nanotechnology‐enabled
against
pandemic,
with
an
emphasis
on
interplay
between
nanotechnology
host
immune
system.
Although
CRISPR-mediated
genome
editing
holds
promise
for
cancer
therapy,
inadequate
tumor
targeting
and
potential
off-target
side
effects
hamper
its
outcomes.
In
this
study,
we
present
a
strategy
using
cryo-shocked
lung
cells
as
CRISPR-Cas9
delivery
system
cyclin-dependent
kinase
4
(
CDK4
)
gene
editing,
which
initiates
synthetic
lethal
in
KRAS-mutant
non–small
cell
(NSCLC).
By
rapidly
liquid
nitrogen
shocking,
effectively
eliminate
the
pathogenicity
of
while
preserving
their
structure
surface
receptor
activity.
This
enables
loaded
to
efficiently
target
through
capture
pulmonary
capillaries
interactions
with
endothelial
cells.
NSCLC-bearing
mouse
model,
drug
accumulation
is
increased
nearly
fourfold
lung,
intratumoral
expression
substantially
down-regulated
compared
lipofectamine
nanoparticles
administration.
Furthermore,
editing–mediated
ablation
triggers
NSCLC
prolongs
survival
mice.
Abstract
Surface
bio‐engineering
of
polymeric
nanoparticles
(PNPs)
has
emerged
as
a
cornerstone
in
contemporary
biomedical
research,
presenting
transformative
avenue
that
can
revolutionize
diagnostics,
therapies,
and
drug
delivery
systems.
The
approach
involves
integrating
bioactive
elements
on
the
surfaces
PNPs,
aiming
to
provide
them
with
functionalities
enable
precise,
targeted,
favorable
interactions
biological
components
within
cellular
environments.
However,
full
potential
surface
bio‐engineered
PNPs
biomedicine
is
hampered
by
obstacles,
including
precise
control
over
modifications,
stability
environments,
lasting
targeted
cells
or
tissues.
Concerns
like
scalability,
reproducibility,
long‐term
safety
also
impede
translation
clinical
practice.
In
this
review,
these
challenges
context
recent
breakthroughs
developing
surface‐biofunctionalized
for
various
applications,
from
biosensing
bioimaging
therapeutics
are
discussed.
Particular
attention
given
bonding
mechanisms
underlie
attachment
moieties
PNP
surfaces.
efficacy
surface‐bioengineered
critically
reviewed
disease
detection,
treatment,
both
vitro
vivo
settings.
Insights
into
existing
limitations
impeding
progress
provided,
forward‐looking
discussion
field's
future
presented.
paper
concludes
recommendations
accelerate
PNPs.
Journal of Medical Virology,
Год журнала:
2025,
Номер
97(2)
Опубликована: Фев. 1, 2025
ABSTRACT
The
term
“Disease
X”,
first
introduced
by
the
World
Health
Organization
(WHO)
in
2018,
symbolizes
threat
of
an
unknown
pathogen
capable
causing
a
global
pandemic.
Classified
as
“priority
pathogens,”
Disease
X
stands
alongside
well‐known
threats
like
SARS,
Ebola,
and
ZIKV
due
to
its
potential
for
widespread
outbreaks.
SARS‐CoV‐2
is
considered
X”
fulfill
this
prediction,
demonstrating
devastating
impact
such
pathogens
can
have.
A
future
could
pose
even
greater
threat,
with
catastrophic
consequences.
This
paper
examines
origins
pathogens,
drawing
lessons
from
outbreaks
MERS,
SARS‐CoV‐2.
It
also
highlights
strategic
approaches
detect,
prevent,
respond
effectively
mitigate
risk
pandemics.
Molecular Therapy — Methods & Clinical Development,
Год журнала:
2025,
Номер
33(1), С. 101436 - 101436
Опубликована: Фев. 16, 2025
Lipid
nanoparticles
(LNPs)
are
now
highly
effective
transporters
of
nucleic
acids
to
the
liver.
This
liver-specificity
is
largely
due
their
association
with
certain
serum
proteins,
most
notably
apolipoprotein
E
(ApoE),
which
directs
them
liver
cells
by
binding
low-density
lipoprotein
(LDL)
receptors
on
hepatocytes.
The
liver's
distinct
anatomy,
its
various
specialized
cell
types,
also
influences
how
LNPs
taken
up
from
circulation,
cleared,
and
they
in
delivering
treatments.
In
this
review,
we
consider
factors
that
facilitate
LNP's
targeting
explore
latest
advances
liver-targeted
LNP
technologies.
Understanding
targeted
can
help
for
design
optimization
nanoparticle-based
therapies.
Comprehension
cellular
interaction
biodistribution
not
only
leads
better
treatments
diseases
but
delivers
insight
directing
other
tissues,
potentially
broadening
range
therapeutic
applications.
Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology,
Год журнала:
2024,
Номер
16(4)
Опубликована: Июль 1, 2024
Abstract
mRNA
therapeutics
have
shown
great
potential
for
a
broad
spectrum
of
disease
treatment.
However,
the
challenges
mRNA's
inherent
instability
and
difficulty
in
cellular
entry
hindered
its
progress
biomedical
field.
To
address
barriers
deliver
to
cells
interest,
various
delivery
systems
are
designed.
Among
these,
lipid
nanoparticles
(LNPs)
stand
out
as
most
extensively
used
systems,
particularly
following
clinical
approvals
corona
virus
2019
(COVID‐19)
vaccines.
LNPs
comprised
ionizable
cationic
lipids,
phospholipids,
cholesterol,
polyethylene
glycol
derived
lipids
(PEG‐lipids).
In
this
review,
we
primarily
summarize
recent
advancements
LNP
technology,
focusing
on
structures
four
constituents
their
applications.
We
delve
into
structure–activity
relationships
while
also
exploring
future
prospects
developing
more
efficacious
systems.
This
article
is
categorized
under:
Therapeutic
Approaches
Drug
Discovery
>
Emerging
Technologies
Biology‐Inspired
Nanomaterials
Lipid‐Based
Structures
Nanotechnology
Biology
Nanoscale
Systems
