Inside-out assembly of viral antigens for the enhanced vaccination DOI Creative Commons
Fengqiang Cao, Sha Peng,

Yaling An

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)

Published: May 24, 2023

Abstract Current attempts in vaccine delivery systems concentrate on replicating the natural dissemination of live pathogens, but neglect that pathogens evolve to evade immune system rather than provoke it. In case enveloped RNA viruses, it is nucleocapsid protein (NP, core antigen) and surface antigen delays NP exposure surveillance. Here, we report a multi-layered aluminum hydroxide-stabilized emulsion (MASE) dictate sequence antigens. this manner, receptor-binding domain (RBD, spike was trapped inside nanocavity, while absorbed outside droplets, enabling burst release before RBD. Compared with packaging strategy, inside-out strategy induced potent type I interferon-mediated innate responses triggered an immune-potentiated environment advance, which subsequently boosted CD40 + DC activations engagement lymph nodes. both H1N1 influenza SARS-CoV-2 vaccines, rMASE significantly increased antigen-specific antibody secretion, memory T cell engagement, Th1-biased response, diminished viral loads after lethal challenge. By simply reversing antigen, may offer major implications for enhanced vaccinations against virus.

Language: Английский

Lipid-Based Nanoparticles for Drug/Gene Delivery: An Overview of the Production Techniques and Difficulties Encountered in Their Industrial Development DOI Creative Commons
Meenu Mehta, Thuỳ Anh Bùi,

Xinpu Yang

et al.

ACS Materials Au, Journal Year: 2023, Volume and Issue: 3(6), P. 600 - 619

Published: Aug. 21, 2023

Over the past decade, therapeutic potential of nanomaterials as novel drug delivery systems complementing conventional pharmacology has been widely acknowledged. Among these nanomaterials, lipid-based nanoparticles (LNPs) have shown remarkable pharmacological performance and promising outcomes, thus gaining substantial interest in preclinical clinical research. In this review, we introduce main types LNPs used formulations such liposomes, nanoemulsions, solid lipid nanoparticles, nanostructured carriers, polymer hybrid focusing on their physicochemical properties potential. We discuss computational studies modeling techniques to enhance understanding how interact with cargo predict effectiveness interactions applications. also analyze benefits drawbacks various LNP production nanoprecipitation, emulsification, evaporation, thin film hydration, microfluidic-based methods, an impingement jet mixer. Additionally, major challenges associated industrial development, including stability sterilization, storage, regulatory compliance, reproducibility, quality control. Overcoming facilitating compliance represent key steps toward LNP's successful commercialization translation into settings.

Language: Английский

Citations

179
Continuous Spatiotemporal Therapy of A Full-API Nanodrug via Multi-Step Tandem Endogenous Biosynthesis DOI Creative Commons
Fang Fang, Sa Wang,

Yueyue Song

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 25, 2023

Abstract Nanomedicine holds great promise to enhance cancer therapy. However, low active pharmaceutical ingredient (API) loading content, unpredictable drug release, and potential toxicity from excipients limit their translational capability. We herein report a full-API nanodrug composed of FDA-approved 5-aminolevulinic acid (ALA), human essential element Fe 3+ , natural bioactive compound curcumin with an ideal API content pH-responsive release profile for continuous spatiotemporal therapy achieved by multi-step tandem endogenous biosynthesis. First, ALA enzymatically converts into photosensitizer protoporphyrin IX (PpIX). Afterward, multiple downstream products including carbon monoxide (CO), 2+ biliverdin (BV), bilirubin (BR) are individually biosynthesized through the PpIX-heme-CO/Fe /BV-BR metabolic pathway, further cooperating released curcumin, ultimately eliciting mitochondria damage, membrane disruption, intracytoplasmic injury. This work not only provides paradigm exploiting diversified metabolites tumor suppression, but also presents safe efficient nanodrug, facilitating practical translation nanodrugs.

Language: Английский

Citations

56
Aggregation-Induced Emission Luminogen: Role in Biopsy for Precision Medicine DOI Creative Commons
Yanhong Duo, Lei Han, Yaoqiang Yang

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(20), P. 11242 - 11347

Published: Oct. 9, 2024

Biopsy, including tissue and liquid biopsy, offers comprehensive real-time physiological pathological information for disease detection, diagnosis, monitoring. Fluorescent probes are frequently selected to obtain adequate on processes in a rapid minimally invasive manner based their advantages biopsy. However, conventional fluorescent have been found show aggregation-caused quenching (ACQ) properties, impeding greater progresses this area. Since the discovery of aggregation-induced emission luminogen (AIEgen) promoted advancements molecular bionanomaterials owing unique high quantum yield (QY) signal-to-noise ratio (SNR),

Language: Английский

Citations

19
Nanotechnology‐facilitated vaccine development during the coronavirus disease 2019 (COVID‐19) pandemic DOI
Ziqi Wang, Kai Cui, Ulrich Costabel

et al.

Exploration, Journal Year: 2022, Volume and Issue: 2(5)

Published: July 21, 2022

Abstract Coronavirus disease 2019 (COVID‐19) continually poses a significant threat to the human race, and prophylactic vaccination is most potent approach end this pandemic. Nanotechnology widely adopted during COVID‐19 vaccine development, engineering of nanostructured materials such as nanoparticles has opened new possibilities in innovative development by improving design accelerating process. This review aims comprehensively understand current situation prospects nanotechnology‐enabled against pandemic, with an emphasis on interplay between nanotechnology host immune system.

Language: Английский

Citations

46
Cryo-shocked tumor cells deliver CRISPR-Cas9 for lung cancer regression by synthetic lethality DOI Creative Commons
F Liu, Minhang Xin,

Huiheng Feng

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(13)

Published: March 29, 2024

Although CRISPR-mediated genome editing holds promise for cancer therapy, inadequate tumor targeting and potential off-target side effects hamper its outcomes. In this study, we present a strategy using cryo-shocked lung cells as CRISPR-Cas9 delivery system cyclin-dependent kinase 4 ( CDK4 ) gene editing, which initiates synthetic lethal in KRAS-mutant non–small cell (NSCLC). By rapidly liquid nitrogen shocking, effectively eliminate the pathogenicity of while preserving their structure surface receptor activity. This enables loaded to efficiently target through capture pulmonary capillaries interactions with endothelial cells. NSCLC-bearing mouse model, drug accumulation is increased nearly fourfold lung, intratumoral expression substantially down-regulated compared lipofectamine nanoparticles administration. Furthermore, editing–mediated ablation triggers NSCLC prolongs survival mice.

Language: Английский

Citations

15
Optimized lipid nanoparticles (LNPs) for organ-selective nucleic acids delivery in vivo DOI Creative Commons
Tian Zhang,

Han Yin,

Li Yu

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 109804 - 109804

Published: April 23, 2024

Nucleic acid therapeutics offer tremendous promise for addressing a wide range of common public health conditions. However, the

Language: Английский

Citations

13
Surface Bio‐engineered Polymeric Nanoparticles DOI Creative Commons
Laura Libnan Haidar, M.M.M. Bilek, Behnam Akhavan

et al.

Small, Journal Year: 2024, Volume and Issue: 20(21)

Published: Feb. 23, 2024

Abstract Surface bio‐engineering of polymeric nanoparticles (PNPs) has emerged as a cornerstone in contemporary biomedical research, presenting transformative avenue that can revolutionize diagnostics, therapies, and drug delivery systems. The approach involves integrating bioactive elements on the surfaces PNPs, aiming to provide them with functionalities enable precise, targeted, favorable interactions biological components within cellular environments. However, full potential surface bio‐engineered PNPs biomedicine is hampered by obstacles, including precise control over modifications, stability environments, lasting targeted cells or tissues. Concerns like scalability, reproducibility, long‐term safety also impede translation clinical practice. In this review, these challenges context recent breakthroughs developing surface‐biofunctionalized for various applications, from biosensing bioimaging therapeutics are discussed. Particular attention given bonding mechanisms underlie attachment moieties PNP surfaces. efficacy surface‐bioengineered critically reviewed disease detection, treatment, both vitro vivo settings. Insights into existing limitations impeding progress provided, forward‐looking discussion field's future presented. paper concludes recommendations accelerate PNPs.

Language: Английский

Citations

12
Why Do Lipid Nanoparticles Target the Liver? Understanding of Biodistribution and Liver-Specific Tropism DOI Creative Commons
Mahboubeh Hosseini-Kharat, Kristen E. Bremmell, Clive A. Prestidge

et al.

Molecular Therapy — Methods & Clinical Development, Journal Year: 2025, Volume and Issue: 33(1), P. 101436 - 101436

Published: Feb. 16, 2025

Lipid nanoparticles (LNPs) are now highly effective transporters of nucleic acids to the liver. This liver-specificity is largely due their association with certain serum proteins, most notably apolipoprotein E (ApoE), which directs them liver cells by binding low-density lipoprotein (LDL) receptors on hepatocytes. The liver's distinct anatomy, its various specialized cell types, also influences how LNPs taken up from circulation, cleared, and they in delivering treatments. In this review, we consider factors that facilitate LNP's targeting explore latest advances liver-targeted LNP technologies. Understanding targeted can help for design optimization nanoparticle-based therapies. Comprehension cellular interaction biodistribution not only leads better treatments diseases but delivers insight directing other tissues, potentially broadening range therapeutic applications.

Language: Английский

Citations

1
An overview of lipid constituents in lipid nanoparticle mRNA delivery systems DOI

Shiqi Wu,

Lixin Lin, Lu Shi

et al.

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2024, Volume and Issue: 16(4)

Published: July 1, 2024

Abstract mRNA therapeutics have shown great potential for a broad spectrum of disease treatment. However, the challenges mRNA's inherent instability and difficulty in cellular entry hindered its progress biomedical field. To address barriers deliver to cells interest, various delivery systems are designed. Among these, lipid nanoparticles (LNPs) stand out as most extensively used systems, particularly following clinical approvals corona virus 2019 (COVID‐19) vaccines. LNPs comprised ionizable cationic lipids, phospholipids, cholesterol, polyethylene glycol derived lipids (PEG‐lipids). In this review, we primarily summarize recent advancements LNP technology, focusing on structures four constituents their applications. We delve into structure–activity relationships while also exploring future prospects developing more efficacious systems. This article is categorized under: Therapeutic Approaches Drug Discovery > Emerging Technologies Biology‐Inspired Nanomaterials Lipid‐Based Structures Nanotechnology Biology Nanoscale Systems

Language: Английский

Citations

8
COVID-19 Vaccines, Effectiveness, and Immune Responses DOI Open Access

Haneen I. Abufares,

Leen Oyoun Alsoud,

Mohammad A. Y. Alqudah

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(23), P. 15415 - 15415

Published: Dec. 6, 2022

The COVID-19 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has captivated globe's attention since its emergence in 2019. This highly infectious, spreadable, and dangerous pathogen health, social, economic crises. Therefore, a worldwide collaborative effort was made to find an efficient strategy overcome develop vaccines. new vaccines provide effective immune response that safeguards community from virus' severity. WHO approved nine for emergency use based on safety efficacy data collected various conducted clinical trials. Herein, we review effectiveness of WHO-approved associated responses, their impact improving public's health. Several immunological studies have demonstrated vaccination dramatically enhances reduces likelihood future infections previously infected individuals. However, type individual health status can significantly affect responses. Exposure healthy individuals adenovirus vectors or mRNA causes early production antibodies B T cells. On other hand, unhealthy were more likely experience harmful events due relapses existing conditions. Taken together, aligning with proper patient's case result better outcomes.

Language: Английский

Citations

28