Genetically Engineered Bacteria as A Living Bioreactor for Monitoring and Elevating Hypoxia‐Activated Prodrug Tumor Therapy DOI
Hongwei Zhang, Linfu Chen, Qian Chen

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 14, 2024

Abstract Tirapazamine (TPZ), an antitumor prodrug, can be activated in hypoxic environment. It specifically targets the microenvironment of tumors and produces toxic free radicals. However, due to tumor is not completely hypoxic, TPZ often fails effectively treat entire tissue, resulting suboptimal therapeutic outcomes. Herein, a low pathogenic Escherichia coli TOP10 utilized selectively colonize tissues, disrupt blood vessels, induce thrombus formation, leading expansion region improving effect TPZ. Additionally, thermosensitive hydrogel constructed by Pluronic F‐127 (F127), which undergoes gelation situ at site, sustained release To monitor process, it genetically modified integrating bioluminescent system luxCDABE (TOP10‐Lux). The signal associated with hypoxia enhancement beneficial for monitoring bioluminescence imaging. In murine colon cancer model, TOP10‐Lux combined TPZ‐loaded F127 suppressed growth, treatment process efficiently monitored. Together, this work employs enhance efficacy providing effective strategy bacteria‐based tumor‐targeted chemotherapy monitoring.

Язык: Английский

Continuous Spatiotemporal Therapy of A Full-API Nanodrug via Multi-Step Tandem Endogenous Biosynthesis DOI Creative Commons
Fang Fang, Sa Wang,

Yueyue Song

и другие.

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Март 25, 2023

Abstract Nanomedicine holds great promise to enhance cancer therapy. However, low active pharmaceutical ingredient (API) loading content, unpredictable drug release, and potential toxicity from excipients limit their translational capability. We herein report a full-API nanodrug composed of FDA-approved 5-aminolevulinic acid (ALA), human essential element Fe 3+ , natural bioactive compound curcumin with an ideal API content pH-responsive release profile for continuous spatiotemporal therapy achieved by multi-step tandem endogenous biosynthesis. First, ALA enzymatically converts into photosensitizer protoporphyrin IX (PpIX). Afterward, multiple downstream products including carbon monoxide (CO), 2+ biliverdin (BV), bilirubin (BR) are individually biosynthesized through the PpIX-heme-CO/Fe /BV-BR metabolic pathway, further cooperating released curcumin, ultimately eliciting mitochondria damage, membrane disruption, intracytoplasmic injury. This work not only provides paradigm exploiting diversified metabolites tumor suppression, but also presents safe efficient nanodrug, facilitating practical translation nanodrugs.

Язык: Английский

Процитировано

57
TROP2-directed nanobody-drug conjugate elicited potent antitumor effect in pancreatic cancer DOI Creative Commons

Caili Xu,

Min Zhu, Qian Wang

и другие.

Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)

Опубликована: Ноя. 6, 2023

Pancreatic cancer is a highly aggressive malignancy with limited treatment options and poor prognosis. Trophoblast cell surface antigen 2 (TROP2), overexpressed in the tumors of more than half pancreatic patients, has been identified as potential target for antibody-drug conjugates (ADCs). Almost all reported TROP2-targeted ADCs are IgG type have poorly studied cancer. Here, we aimed to develop novel nanobody-drug conjugate (NDC) targeting TROP2 cancer.In this study, developed NDC, HuNbTROP2-HSA-MMAE, TROP2-positive HuNbTROP2-HSA-MMAE characterized by use nanobodies against human serum albumin (HSA) drug-antibody ratio 1. exhibited specific binding was internalized into tumor cells high endocytosis efficiency within 5 h, followed intracellular translocation lysosomes release MMAE induce apoptosis through caspase-3/9 pathway. In xenograft model cancer, doses 0.2 mg/kg 1 demonstrated significant antitumor effects, dose even eradicated tumor.HuNbTROP2-HSA-MMAE desirable affinity, internalization activity. It holds promise therapeutic option

Язык: Английский

Процитировано

53
Indocyanine green potentiated paclitaxel nanoprodrugs for imaging and chemotherapy DOI Creative Commons

Xiujuan Xiang,

Xuan Feng,

Shaojin Lu

и другие.

Exploration, Год журнала: 2022, Номер 2(4)

Опубликована: Июнь 4, 2022

Self-assembled prodrug nanoparticles with tumor-responsive capacity have great potential in tumor visualization and treatment. However, the nanoparticle formulas usually contain multiple components, especially polymeric materials, which result various issues. Herein, we report an indocyanine green (ICG)-driven assembly of paclitaxel prodrugs integrating near-infrared fluorescence imaging tumor-specific chemotherapy. By feat hydrophilic merit ICG, dimer could form more uniformly monodispersed nanoparticles. This two-in-one strategy reinforces complementary advantages, resulting superior behavior, robust colloidal stability, enhanced accumulation as well desirable vivo feedback The experiments validated activation at sites evidenced by intensity, potent growth suppression, reduced systemic toxicity compared commercial Taxol. universality ICG potentiated toward photosensitizers dyes was confirmed. presentation provides deep insight into feasibility constructing clinical-close alternatives for improving antitumor efficacy.

Язык: Английский

Процитировано

56
Near-infrared photosensitizers adaptive to tumor hypoxic microenvironment for synergistic photothermal-photodynamic and immunotherapy DOI
Xiangchun Meng, Yufang Han, Shaobo Wang

и другие.

Nano Today, Год журнала: 2023, Номер 53, С. 102030 - 102030

Опубликована: Окт. 21, 2023

Язык: Английский

Процитировано

25
Endoperoxide‐enhanced self‐assembled ROS producer as intracellular prodrugs for tumor chemotherapy and chemodynamic therapy DOI Creative Commons
Junjie Tang, Yadong Liu, Yifan Xue

и другие.

Exploration, Год журнала: 2024, Номер 4(4)

Опубликована: Фев. 9, 2024

Abstract Prodrug‐based self‐assembled nanoparticles (PSNs) with tailored responses to tumor microenvironments show a significant promise for chemodynamic therapy (CDT) by generating highly toxic reactive oxygen species (ROS). However, the insufficient level of intracellular ROS and limited drug accumulation remain major challenges further clinical transformation. In this study, PSNs delivery artesunate (ARS) are demonstrated designing pH‐responsive ARS‐4‐hydroxybenzoyl hydrazide (HBZ)‐5‐amino levulinic acid (ALA) (AHA NPs) self‐supplied excellent chemotherapy CDT. The greatly improved loading capacity generation from endoperoxide bridge using electron withdrawing group attached directly C10 site artesunate. ALA ARS‐HBZ could be released AHA NPs under cleavage hydrazone bonds triggered acidic surroundings. Besides, increased heme in mitochondria, promoting lipid peroxidation anti‐tumor effects. Our study ARS through chemical modification, pointing out potential applications fields.

Язык: Английский

Процитировано

15
Nanoparticles for the Treatment of Bone Metastasis in Breast Cancer: Recent Advances and Challenges DOI Creative Commons
Xianzhe Yu, Lingling Zhu

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 1867 - 1886

Опубликована: Фев. 1, 2024

Abstract: Although the frequency of bone metastases from breast cancer has increased, effective treatment is lacking, prompting development nanomedicine, which involves use nanotechnology for disease diagnosis and treatment. Nanocarrier drug delivery systems offer several advantages over traditional methods, such as higher reliability biological activity, improved penetration retention, precise targeting delivery. Various nanoparticles that can selectively target tumor cells without causing harm to healthy or organs have been synthesized. Recent advances in enabled prevention metastatic diseases well ability deliver complex molecular "cargo" particles regions. Nanoparticles modulate systemic biodistribution enable targeted accumulation therapeutic agents. Several strategies are used treat metastases, including untargeted delivery, bone-targeted cell-targeted Combining agents with enhances selective payloads lesions, providing multiple In this review, we describe recent nanoparticle treating metastases. Keywords: cancer, metastasis, nanoparticle,

Язык: Английский

Процитировано

14
Self‐Carrier Nanoparticles for Delivery of Paclitaxel and IDO Inhibitor to Boost Antitumor Chemo‐Immunotherapy DOI
Zixuan Li,

Qing Pei,

Min Zhao

и другие.

Advanced Functional Materials, Год журнала: 2024, Номер 34(30)

Опубликована: Март 21, 2024

Abstract The combination of chemotherapy and immunotherapy holds great potential in clinical treatment advanced cancers. Whereas, the therapeutic outcome chemotherapeutic immune regulator is suboptimal due to their poor tumor availability severe off‐target toxicity. Herein, self‐carrier nanoparticles (PSMT NPs) integrating a paclitaxel (PTX) prodrug indoleamine 2,3‐dioxygenase 1 (IDO) inhibitor (1‐methyl‐tryptophan, 1MT) for tumor‐specific chemo‐immunotherapy constructed. After internalization by cancer cells, PSMT NPs can respond endogenous redox stimulus, release PTX 1MT. released not only promote cell apoptosis via intervention mitosis but also initiate immunogenic death facilitate recruitment activation tumor‐infiltrating cytotoxic T lymphocytes. concomitant 1MT inhibit IDO activity exhaust regulatory thereby synergistically activating exhibit potentiated antitumor output toward triple‐negative breast negligible systemic This facile versatile nanoplatform provides promising strategy cooperatively activate immunity chemo‐immunotherapy.

Язык: Английский

Процитировано

12
Tailoring Indocyanine Green J-Aggregates for Imaging, Cancer Phototherapy, and Drug Delivery: A Review DOI
Pooja Kumari, Smriti Arora,

Yuanwei Pan

и другие.

ACS Applied Bio Materials, Год журнала: 2024, Номер 7(8), С. 5121 - 5135

Опубликована: Июль 23, 2024

Indocyanine green J-aggregates (ICG-Jagg) have emerged as a significant subject of interest in biomedical applications due to their unique optical properties, tunable size, and excellent biocompatibility. This comprehensive review aims provide an in-depth exploration ICG-Jagg, with focus on elucidating the diverse facets preparation factors that influence process. Additionally, discusses diagnostics, such imaging contrast agents, well utilization drug delivery various phototherapeutic interventions.

Язык: Английский

Процитировано

11
A supramolecular assembly strategy for hydrophilic drug delivery towards synergistic cancer treatment DOI

Haijing Qu,

Han Chen, Wei Cheng

и другие.

Acta Biomaterialia, Год журнала: 2023, Номер 164, С. 407 - 421

Опубликована: Апрель 23, 2023

Язык: Английский

Процитировано

20
Microenvironment Restruction of Emerging 2D Materials and their Roles in Therapeutic and Diagnostic Nano‐Bio‐Platforms DOI Creative Commons
Qian Li, Xizheng Wu, Shengdong Mu

и другие.

Advanced Science, Год журнала: 2023, Номер 10(20)

Опубликована: Май 2, 2023

Engineering advanced therapeutic and diagnostic nano-bio-platforms (NBPFs) have emerged as rapidly-developed pathways against a wide range of challenges in antitumor, antipathogen, tissue regeneration, bioimaging, biosensing applications. Emerged 2D materials attracted extensive scientific interest fundamental building blocks or nanostructures among material scientists, chemists, biologists, doctors due to their advantageous physicochemical biological properties. This timely review provides comprehensive summary creating NBPFs via emerging (2D-NBPFs) with unique insights into the corresponding molecularly restructured microenvironments biofunctionalities. First, it is focused on an up-to-date overview synthetic strategies for designing 2D-NBPFs cross-comparison advantages disadvantages. After that, recent key achievements are summarized tuning biofunctionalities programmed microenvironments, including physiological stability, biocompatibility, bio-adhesiveness, specific binding pathogens, broad-spectrum pathogen inhibitors, stimuli-responsive systems, enzyme-mimetics. Moreover, representative applications also discussed detailed disclosure critical design principles parameters. Finally, current future research directions discussed. Overall, this will provide cutting-edge multidisciplinary guidance accelerating developments therapeutic/diagnostic 2D-NBPFs.

Язык: Английский

Процитировано

19