Whole-brain analysis of CO2chemosensitive regions and identification of the retrotrapezoid and medullary raphé nuclei in the common marmoset (Callithrix jacchus) DOI Creative Commons

Ariana Z. Turk,

Marissa Millwater,

Shahriar SheikhBahaei

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 28, 2023

Respiratory chemosensitivity is an important mechanism by which the brain senses changes in blood partial pressure of CO2 (PCO2). It proposed that special neurons (and astrocytes) various brainstem regions play key roles as central respiratory chemosensors rodents. Although common marmosets (Callithrix jacchus), New-World non-human primates, show similar responses to elevated inspired rodents, chemosensitive marmoset have not been defined yet. Here, we used c-fos immunostainings identify brain-wide CO2-activated marmosets. In addition, mapped location retrotrapezoid nucleus (RTN) and raphé nuclei based on colocalization CO2-induced immunoreactivity with Phox2b, TPH immunostaining, respectively. Our data also indicated that, RTN astrocytes express Phox2b complex processes create a meshwork structure at ventral surface medulla. highlight some cellular structural regional similarities

Язык: Английский

Astrocytes in memory formation and maintenance DOI
Kirsten Bohmbach, Christian Henneberger, Johannes Hirrlinger

и другие.

Essays in Biochemistry, Год журнала: 2022, Номер 67(1), С. 107 - 117

Опубликована: Сен. 23, 2022

Learning and memory are fundamental but highly complex functions of the brain. They rely on multiple mechanisms including processing sensory information, formation, maintenance short- long-term memory, retrieval extinction. Recent experiments provide strong evidence that, besides neurons, astrocytes crucially contribute to these higher brain functions. However, interplay neurons in local neuron-glia assemblies is far from being understood. Although important basic cellular principles that govern link neuronal astrocytic have been established, additional clearly continue emerge. In this short essay, we first review current technologies allowing experimenter explore role behaving animals, with focus spatial memory. We then discuss signaling their learning also reveal gaps our knowledge currently prevent a comprehensive understanding how acquisition, storage by modulating circuits.

Язык: Английский

Процитировано

25

Contributions of carotid bodies, retrotrapezoid nucleus neurons and preBötzinger complex astrocytes to the CO2‐sensitive drive for breathing DOI Creative Commons
Shahriar SheikhBahaei, Nephtalı́ Marina, Vishaal Rajani

и другие.

The Journal of Physiology, Год журнала: 2023, Номер 602(1), С. 223 - 240

Опубликована: Сен. 24, 2023

Abstract Current models of respiratory CO 2 chemosensitivity are centred around the function a specific population neurons residing in medullary retrotrapezoid nucleus (RTN). However, there is significant evidence suggesting that chemosensitive exist other brainstem areas, including rhythm‐generating region medulla oblongata – preBötzinger complex (preBötC). There also astrocytes, non‐neuronal brain cells, contribute to central chemosensitivity. In this study, we reevaluated relative contributions RTN neurons, preBötC and carotid body chemoreceptors mediating responses experimental animals (adult laboratory rats). To block astroglial signalling via exocytotic release transmitters, astrocytes were targeted express tetanus toxin light chain (TeLC). Bilateral expression TeLC was associated with ∼20% ∼30% reduction response conscious anaesthetized animals, respectively. Carotid denervation reduced by ∼25%. inhibition transduced Gi‐coupled designer receptors exclusively activated drug (DREADD Gi ) application clozapine‐ N ‐oxide ∼40% rats, Combined blockade preBötC, ‐induced ∼70%. These data further support hypothesis ‐sensitive drive breathe requires inputs from peripheral several chemoreceptor sites. At level, modulate activity network , either relaying chemosensory information (i.e. they act as sensors) or enhancing excitability inputs. image Key points This study roles played bodies, (RTN) breathe. The obtained show disruption signalling, similarly reduce hypercapnia. provide for mediated

Язык: Английский

Процитировано

10

Temporal variations in the pattern of breathing: techniques, sources, and applications to translational sciences DOI Creative Commons
Yoshitaka Oku

The Journal of Physiological Sciences, Год журнала: 2022, Номер 72(1)

Опубликована: Авг. 29, 2022

Abstract The breathing process possesses a complex variability caused in part by the respiratory central pattern generator brainstem; however, it also arises from chemical and mechanical feedback control loops, network reorganization sharing with nonrespiratory motor acts, as well inputs cortical subcortical systems. notion that fluctuations contain hidden information has prompted scientists to decipher signals better understand fundamental mechanisms of generation, interactions emotion, influences on neuronal networks associated cognition, changes healthy disease-carrying individuals. Respiration can be used express emotion. Furthermore, respiration appears organize brain-wide oscillations via cross-frequency coupling, optimizing cognitive performance. With aid theory-based techniques machine learning, translated into form usable clinical practice for diagnosis, emotion recognition, mental conditioning.

Язык: Английский

Процитировано

11

Morphological deficits of glial cells in a transgenic mouse model for developmental stuttering DOI Open Access
Afuh Adeck,

Marissa Millwater,

Camryn Bragg

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 5, 2024

Vocal production involves intricate neural coordination across various brain regions. Stuttering, a common speech disorder, has genetic underpinnings, including mutations in lysosomal-targeting pathway genes. Using Gnptab-mutant mouse model linked to stuttering, we examined neuron and glial cell morphology vocal circuits. Our findings revealed altered astrocyte microglia processes these circuits mice, while control regions remained unaffected. results shed light on the potential role of cells stuttering pathophysiology highlight their relevance modulating behaviors.

Язык: Английский

Процитировано

2

Insulin and leptin acutely modulate the energy metabolism of primary hypothalamic and cortical astrocytes DOI Creative Commons
C Wolff, Dorit John,

Ulrike Winkler

и другие.

Journal of Neurochemistry, Год журнала: 2024, Номер 169(1)

Опубликована: Авг. 22, 2024

Astrocytes constitute a heterogeneous cell population within the brain, contributing crucially to brain homeostasis and playing an important role in overall function. Their function metabolism are not only regulated by local signals, for example, from nearby neurons, but also long-range signals such as hormones. Thus, two prominent hormones primarily known regulating energy balance of whole organism, insulin, leptin, have been reported impact astrocytes brain. In this study, we investigated acute regulation astrocytic these cultured prepared mouse cortex hypothalamus, pivotal region context nutritional regulation. Utilizing genetically encoded, fluorescent nanosensors, cytosolic concentrations glucose, lactate, ATP, along with glycolytic rate NADH/NAD

Язык: Английский

Процитировано

1

The Masticatory Activity Interference in Quantitative Estimation of CA1, CA3 and Dentate Gyrus Hippocampal Astrocytes of Aged Murine Models and under Environmental Stimulation DOI Open Access
Marília da Cunha Feio Leal, Fabio Leite do Amaral, Bernardo Freire da Silva Arruda

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(7), С. 6529 - 6529

Опубликована: Март 31, 2023

Studies indicating the influence of masticatory dysfunction, due to a soft diet or lack molars, on impairing spatial memory and learning have led research about neuronal connections between areas cell populations possibly affected. In this sense, with scarce detailed data subfields hippocampus in dementia neurodegeneration, there is no information astrocytic responses its different layers. Thus, considering context, present study evaluated effects deprivation rehabilitation activity, aging, environmental enrichment stereological quantification hippocampal astrocytes from layers CA1, CA3, DG. For purpose, we examined mature (6-month-old; 6M), aged (18-month-old; 18M) mice, subjected distinct regimens environments. Three activity were applied: continuous normal mastication hard pellets (HD); followed by deprived equal periods powder (HD/SD); rehabilitated HD, powder, (HD/SD/HD). Under each specific regimen, half animals raised standard cages (impoverished environment (IE)) other enriched (enriched (EE)), mimicking sedentary active lifestyles. Microscopic stereological, systematic, random sampling approaches an optical dissector GFAP-immunolabeled done, allowing for astrocyte numerical estimate. Stratum moleculare hilus, dentate gyrus (DG) Strata Lacunosum-Moleculare, Oriens, Radiatum, similarly gyrus, showed significant change any investigated variables (age, diet, environment) these However, radiatum, it was possible observe differences associated age. Therefore, diet-related found when HD 18M IE group compared HD/SD/HD 18-month-old same (IE) (p = 0.007). study, modulatory factors (masticatory function, enrichment, aging) differentiated quantitative laminar response regions, suggesting studies read plasticity responsiveness astrocytes, including molecular background.

Язык: Английский

Процитировано

2

Unified Neuromechanical Control Model for Rhythmic and Discrete Hand Movements DOI Creative Commons

Nafiseh Ghaffar Nia,

Fariba Bahrami, Erkan Kaplanoğlu

и другие.

Mathematical Modelling and Engineering Problems, Год журнала: 2024, Номер 11(2), С. 279 - 289

Опубликована: Фев. 27, 2024

Movement is one of the essential characteristics living beings.Despite diversity animal species and apparent differences, standard features exist between their movement systems that follow a particular pattern.The movements can mainly be divided into discrete rhythmic categories controlled by central nervous system.Scientists usually consider these two types motion separately in control system use different methods resources to produce model them.Proposing unified comprehensive for generating controlling with same more valuable, albeit challenging.This because such would address fundamental problem field motor control, offering holistic solution understanding how beings generate movement.A could revolutionize various fields, including robotics, rehabilitation, neuroscience, providing versatile framework applicable applications.In this study, we employed Hodgkin-Huxley (HH) equations our computational model; suitability lies ability capture intricate dynamics neural oscillations behavior networks, making them an ideal choice investigation.Our analysis model, factors influencing motion, oscillation revealed crucial insights.We found supraspinal input neuron feedback, as key parameters, play pivotal roles movements.These findings contribute orchestrates both within single framework.Developing neuromechanical capable creating holds promising implications.This research potentially advance fields biomechanics, rehabilitation control.Moreover, switching mechanism states lead innovative strategies designing adaptive robotic improving protocols individuals impairments.

Язык: Английский

Процитировано

0

The hypoxic respiratory response of the pre-Bötzinger complex DOI Creative Commons
Jamal Khalilpour, Hamid Soltani Zangbar, Mohammad Reza Alipour

и другие.

Heliyon, Год журнала: 2024, Номер 10(14), С. e34491 - e34491

Опубликована: Июль 1, 2024

Язык: Английский

Процитировано

0

Whole-brain analysis of CO2chemosensitive regions and identification of the retrotrapezoid and medullary raphé nuclei in the common marmoset (Callithrix jacchus) DOI Creative Commons

Ariana Z. Turk,

Marissa Millwater,

Shahriar SheikhBahaei

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 28, 2023

Respiratory chemosensitivity is an important mechanism by which the brain senses changes in blood partial pressure of CO2 (PCO2). It proposed that special neurons (and astrocytes) various brainstem regions play key roles as central respiratory chemosensors rodents. Although common marmosets (Callithrix jacchus), New-World non-human primates, show similar responses to elevated inspired rodents, chemosensitive marmoset have not been defined yet. Here, we used c-fos immunostainings identify brain-wide CO2-activated marmosets. In addition, mapped location retrotrapezoid nucleus (RTN) and raphé nuclei based on colocalization CO2-induced immunoreactivity with Phox2b, TPH immunostaining, respectively. Our data also indicated that, RTN astrocytes express Phox2b complex processes create a meshwork structure at ventral surface medulla. highlight some cellular structural regional similarities

Язык: Английский

Процитировано

0