Co‐aggregation as A Simple Strategy for Preparing Fluorogenic Tetrazine Probes with On‐Demand Fluorogen Selection** DOI Open Access
S. Segawa, Jiajie Wu, Ryan T. K. Kwok

и другие.

Angewandte Chemie, Год журнала: 2023, Номер 136(11)

Опубликована: Дек. 7, 2023

Abstract Life science has progressed with applications of fluorescent probes—fluorophores linked to functional units responding biological events. To meet the varied demands across experiments, simple organic reactions connect fluorophores and have been developed, enabling on‐demand selection fluorophore‐functional unit combinations. However, synthesis requires professional equipment skills, standing as a daunting task for life scientists. In this study, we present simple, fast, convenient strategy probe preparation: co‐aggregation hydrophobic molecules. We focused on tetrazine—a difficult‐to‐prepare yet useful that provides effective bioorthogonal reactivity strong fluorogenicity. Simply mixing tetrazine molecules aggregation‐induced emission (AIE) luminogens in water, is induced, AIE quenched. Subsequent click reaction bioorthogonally turns emission, identifying these coaggregates fluorogenic probes. Thanks fluorogenicity, established new time‐gated fluorescence bioimaging technique distinguish overlapping signals, multi‐organelle imaging two same‐color fluorophores. Our study showcases potential method preparation probes well protocols molecular design principles approach, offering an solution evolving needs research.

Язык: Английский

A novel type of heteroleptic Cu(I) complexes featuring nitrogen-rich tetrazine ligands: Syntheses, Crystal Structures, Spectral properties, Cyclic Voltammetry, and Theoretical Calculations DOI Creative Commons

Adam Sztula,

Antal Péter, Ivan Němec

и другие.

Dalton Transactions, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

The study explores the synthesis, structures, and electronic properties of new copper( i ) complexes with tetrazine-based ligands, revealing their spectral redox behaviors reports on first mononuclear heteroleptic Cu( )–tetrazine complex.

Язык: Английский

Процитировано

0
Co‐aggregation as A Simple Strategy for Preparing Fluorogenic Tetrazine Probes with On‐Demand Fluorogen Selection** DOI
S. Segawa, Jiajie Wu, Ryan T. K. Kwok

и другие.

Angewandte Chemie International Edition, Год журнала: 2023, Номер 63(11)

Опубликована: Дек. 7, 2023

Life science has progressed with applications of fluorescent probes-fluorophores linked to functional units responding biological events. To meet the varied demands across experiments, simple organic reactions connect fluorophores and have been developed, enabling on-demand selection fluorophore-functional unit combinations. However, synthesis requires professional equipment skills, standing as a daunting task for life scientists. In this study, we present simple, fast, convenient strategy probe preparation: co-aggregation hydrophobic molecules. We focused on tetrazine-a difficult-to-prepare yet useful that provides effective bioorthogonal reactivity strong fluorogenicity. Simply mixing tetrazine molecules aggregation-induced emission (AIE) luminogens in water, is induced, AIE quenched. Subsequent click reaction bioorthogonally turns emission, identifying these coaggregates fluorogenic probes. Thanks fluorogenicity, established new time-gated fluorescence bioimaging technique distinguish overlapping signals, multi-organelle imaging two same-color fluorophores. Our study showcases potential method preparation probes well protocols molecular design principles approach, offering an solution evolving needs research.

Язык: Английский

Процитировано

6
Co-aggregation as an Exceptionally Simple Platform to Prepare Fluorogenic Tetrazine Probes with On-Demand Fluorophore Selection DOI Creative Commons
S. Segawa, Jiajie Wu, Ryan T. K. Kwok

и другие.

Опубликована: Авг. 4, 2023

Life science has progressed with applications of fluorescence probes — fluorophores linked to functional units responsive biological events. To meet the varied demands across experiments, simple organic reactions connect and are developed, enabling on-demand selection fluorophore-functional unit combinations. However, synthesis requires professional equipment skills, which hamper life scientists’ research. In this study, we demonstrate that co-aggregation hydrophobic molecules in water can serve as a simple, synthesis-free strategy for probe preparation. We focused on tetrazine difficult-to-prepare yet useful provides effective biorthogonal reactivity strong fluorogenicity connected fluorophores. Simply mixing aggregation-induced emission luminogens (AIEgens) water, is induced, AIEgens quenched. Subsequent click reaction bioorthogonally turns emission, identifying those coaggregates fluorogenic probes. utilized bioorthogonal distinguish overlapping signals time-dependent manner, multi-organelle imaging two same-color Our study showcases potential our method preparation fluorescent well protocols molecular design principles approach, offering an solution evolving needs

Язык: Английский

Процитировано

2
An Active Member of the EFMC: The Division of Medicinal Chemistry and Chemical Biology of the Swiss Chemical Society** DOI Open Access
Yves P. Auberson,

Fides Benfatti,

Dennis Gillingham

и другие.

ChemMedChem, Год журнала: 2023, Номер 18(11)

Опубликована: Апрель 18, 2023

The Division of Medicinal Chemistry and Chemical Biology (DMCCB) the Swiss Society is an active contributor to dynamics European scientific communities. Founded in 1987, it pursues its mission foster relationships among academic industrial members, facilitate exchange by organizing symposia courses, encourage excellence. This article presents DMCCB highlights both offer community participation activities EFMC, Federation for chemistry biology.

Язык: Английский

Процитировано

1
Triazines, tetrazines, and fused ring polyaza systems DOI
Pierre Audebert, Vincent Sol

Progress in heterocyclic chemistry, Год журнала: 2024, Номер unknown, С. 395 - 420

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0
Synthesis of 3-Chloro-6-(4-chloro-3,5-dimethyl-1H-pyrazol-1-yl)pyridazine and Its Deuterated Analogue DOI
Maria S. Ledovskaya, Vladimir V. Voronin

Russian Journal of General Chemistry, Год журнала: 2024, Номер 94(11), С. 2833 - 2840

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

0
Co‐aggregation as A Simple Strategy for Preparing Fluorogenic Tetrazine Probes with On‐Demand Fluorogen Selection** DOI Open Access
S. Segawa, Jiajie Wu, Ryan T. K. Kwok

и другие.

Angewandte Chemie, Год журнала: 2023, Номер 136(11)

Опубликована: Дек. 7, 2023

Abstract Life science has progressed with applications of fluorescent probes—fluorophores linked to functional units responding biological events. To meet the varied demands across experiments, simple organic reactions connect fluorophores and have been developed, enabling on‐demand selection fluorophore‐functional unit combinations. However, synthesis requires professional equipment skills, standing as a daunting task for life scientists. In this study, we present simple, fast, convenient strategy probe preparation: co‐aggregation hydrophobic molecules. We focused on tetrazine—a difficult‐to‐prepare yet useful that provides effective bioorthogonal reactivity strong fluorogenicity. Simply mixing tetrazine molecules aggregation‐induced emission (AIE) luminogens in water, is induced, AIE quenched. Subsequent click reaction bioorthogonally turns emission, identifying these coaggregates fluorogenic probes. Thanks fluorogenicity, established new time‐gated fluorescence bioimaging technique distinguish overlapping signals, multi‐organelle imaging two same‐color fluorophores. Our study showcases potential method preparation probes well protocols molecular design principles approach, offering an solution evolving needs research.

Язык: Английский

Процитировано

0