Revolutionizing biofuel generation: Unleashing the power of CRISPR-Cas mediated gene editing of extremophiles DOI Creative Commons
Diksha Garg, Mahesh Kumar Samota,

Nicholas Kontis

и другие.

Microbiological Research, Год журнала: 2023, Номер 274, С. 127443 - 127443

Опубликована: Июнь 26, 2023

Molecular biology techniques like gene editing have altered the specific genes in micro-organisms to increase their efficiency produce biofuels. This review paper investigates outcomes of Clustered regularly interspaced short palindromic repeats (CRISPR) for extremophilic biofuel. Commercial production biofuel from lignocellulosic waste is limited due various constraints. A potential strategy enhance capability extremophiles gene-editing via CRISPR-Cas technology. The intracellular enzymes cellulase, hemicellulose bacteria, fungi and microalgae has been increased by alteration associated with enzymatic activity thermotolerance. microbes Thermococcus kodakarensis, Thermotoga maritima, Thermus thermophilus, Pyrococcus furiosus Sulfolobus sp. are explored production. conversion biomass into biofuels involves pretreatment, hydrolysis fermentation. challenges off-target effect use also addressed. appropriate regulations required maximize effectiveness while minimizing cleavage, as well total biosafety this technique. latest discovery system should provide a new channel creation microbial biorefineries through site- that might boost generation extremophiles. Overall, study highlights genome methods improve biofuel, opening door more effective environmentally friendly methods.

Язык: Английский

Current applications and future perspective of CRISPR/Cas9 gene editing in cancer DOI Creative Commons
Siwei Wang, Chao Gao, Yi-Min Zheng

и другие.

Molecular Cancer, Год журнала: 2022, Номер 21(1)

Опубликована: Фев. 21, 2022

Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) system provides adaptive immunity against plasmids and phages in prokaryotes. This inspires the development of a powerful genome engineering tool, CRISPR/CRISPR-associated nuclease 9 (CRISPR/Cas9) editing system. Due to its high efficiency precision, CRISPR/Cas9 technique has been employed explore functions cancer-related genes, establish tumor-bearing animal models probe drug targets, vastly increasing our understanding cancer genomics. Here, we review current status gene technology oncological research. We first explain basic principles introduce several new CRISPR-based modes. next detail rapid progress CRISPR screening revealing tumorigenesis, metastasis, resistance mechanisms. In addition, delivery vectors finally demonstrate potential enhance effect adoptive T cell therapy (ACT) reduce adverse reactions.

Язык: Английский

Процитировано

279
CRISPR/Cas: A powerful tool for gene function study and crop improvement DOI Creative Commons
Dangquan Zhang, Zhiyong Zhang, Turgay Ünver

и другие.

Journal of Advanced Research, Год журнала: 2020, Номер 29, С. 207 - 221

Опубликована: Окт. 21, 2020

It is a long-standing goal of scientists and breeders to precisely control gene for studying its function as well improving crop yield, quality, tolerance various environmental stresses. The discovery modification CRISPR/Cas system, nature-occurred editing tool, opens an era precision breeding.In this review, we first introduce the brief history followed mechanism application system on study improvement. Currently, genome has been becoming mature cutting-edge biotechnological tool improvement that already used in many different traits crops, including pathogen resistance, abiotic tolerance, plant development morphology even secondary metabolism fiber development. Finally, point out major issues associating with future research directions.Key Scientific Concepts Review: CRISPR/Cas9 robust powerful targeting individual DNA RNA sequence genome. can be target knockin, knockout replacement monitoring regulating expression at epigenome levels by binding specific sequence. Agrobacterium-mediated method still efficient delivering regents into targeted cells. However, other delivery methods, such virus-mediated method, have developed enhanced potentials CRISPR/Cas9-based PAM requirement offers CRISPR/Cas9-targted genetic loci also limits CRISPR/Cas9. Discovering new Cas proteins modifying current enzymes play important role editing. Developing better methods eliminating off-target effects, finding key/master genes controlling growth two directions

Язык: Английский

Процитировано

259
CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement DOI Open Access
Chao Li, Eleanor J. Brant, Hikmet Budak

и другие.

Journal of Zhejiang University SCIENCE B, Год журнала: 2021, Номер 22(4), С. 253 - 284

Опубликована: Апрель 1, 2021

Язык: Английский

Процитировано

136
Viral Vector-Based Gene Therapy DOI Open Access
Xuedan Li, Yang Le, Zhegang Zhang

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(9), С. 7736 - 7736

Опубликована: Апрель 23, 2023

Gene therapy is a technique involving the modification of an individual’s genes for treating particular disease. The key to effective gene efficient carrier delivery system. Viral vectors that have been artificially modified lose their pathogenicity are used widely as system, with advantages natural high transduction efficiency and stable expression. With decades development, viral vector-based therapies achieved promising clinical outcomes. Currently, three vector strategies based on adeno-associated viruses, adenoviruses, lentiviruses. However, certain challenges, such immunotoxicity “off-target”, continue exist. In present review, above discussed along respective therapeutic applications. addition, major translational challenges encountered in summarized, possible address these also discussed.

Язык: Английский

Процитировано

109
Computational Tools and Resources for CRISPR/Cas Genome Editing DOI Creative Commons
Chao Li, Wen Chu, Rafaqat A. Gill

и другие.

Genomics Proteomics & Bioinformatics, Год журнала: 2022, Номер 21(1), С. 108 - 126

Опубликована: Март 24, 2022

The past decade has witnessed a rapid evolution in identifying more versatile clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) nucleases and their functional variants, as well developing precise CRISPR/Cas-derived genome editors. programmable robust features of the editors provide an effective RNA-guided platform for fundamental life science research subsequent applications diverse scenarios, including biomedical innovation targeted crop improvement. One most essential principles is to guide alterations genomic sequences or genes intended manner without undesired off-target impacts, which strongly depends on efficiency specificity single RNA (sgRNA)-directed recognition DNA sequences. Recent advances empirical scoring algorithms machine learning models have facilitated sgRNA design prediction. In this review, we first briefly introduce different CRISPR/Cas tools that should be taken into consideration achieve specific purposes. Secondly, focus computer-assisted resources are widely used designing sgRNAs analyzing CRISPR/Cas-induced on- mutations. Thirdly, insights limitations available computational would help researchers field further optimization. Lastly, suggest simple but workflow choosing applying web-based editing.

Язык: Английский

Процитировано

86
Mitochondrial dysfunction and neurological disorders: A narrative review and treatment overview DOI
Eman E. Alshial,

Muhammad Idris Abdulghaney,

Al-Hassan Soliman Wadan

и другие.

Life Sciences, Год журнала: 2023, Номер 334, С. 122257 - 122257

Опубликована: Ноя. 8, 2023

Язык: Английский

Процитировано

52
CRISPR/Cas: a powerful tool for designing and improving oil crops DOI
Lijie Li, Dangquan Zhang, Zhiyong Zhang

и другие.

Trends in biotechnology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

21
CRISPR/Cas9-Mediated Gene Therapy for Glioblastoma: A Scoping Review DOI Creative Commons
Emir Begagić, Hakija Bečulić, Nermin Đuzić

и другие.

Biomedicines, Год журнала: 2024, Номер 12(1), С. 238 - 238

Опубликована: Янв. 21, 2024

This scoping review examines the use of CRISPR/Cas9 gene editing in glioblastoma (GBM), a predominant and aggressive brain tumor. Categorizing targets into distinct groups, this explores their roles cell cycle regulation, microenvironmental dynamics, interphase processes, therapy resistance reduction. The complexity CRISPR-Cas9 applications GBM research is highlighted, providing unique insights apoptosis, proliferation, immune responses within tumor microenvironment. studies challenge conventional perspectives on specific genes, emphasizing potential therapeutic implications manipulating key molecular players dynamics. Exploring GBMs yields significant regulation cellular spanning interphase, renewal, migration. Researchers, by precisely targeting uncover orchestration governing growth, differentiation during critical phases cycle. findings underscore technology unraveling complex dynamics microenvironment, offering promising avenues for targeted therapies to curb growth. also outlines addressing GBM, employing target genes associated with chemotherapy resistance, showcasing its transformative effective treatments.

Язык: Английский

Процитировано

19
Foxtail Millet: A New Model for C4 Plants DOI
Renhai Peng, Baohong Zhang

Trends in Plant Science, Год журнала: 2020, Номер 26(3), С. 199 - 201

Опубликована: Дек. 21, 2020

Язык: Английский

Процитировано

90
Heartworm disease – Overview, intervention, and industry perspective DOI Creative Commons
Sandra Noack,

John Harrington,

Douglas S. Carithers

и другие.

International Journal for Parasitology Drugs and Drug Resistance, Год журнала: 2021, Номер 16, С. 65 - 89

Опубликована: Апрель 27, 2021

Dirofilaria immitis, also known as heartworm, is a major parasitic threat for dogs and cats around the world. Because of its impact on health welfare companion animals, heartworm disease huge veterinary economic importance especially in North America, Europe, Asia Australia. Within animal market many different preventive products are available, all which contain active components same drug class, macrocyclic lactones. In addition to compliance issues, such under-dosing or irregular treatment intervals, occurrence drug-resistant heartworms within populations Mississippi River areas adds failure treatments. The objective this review provide an overview disease, summarize current control measures highlight potential new avenues best practices prevention.

Язык: Английский

Процитировано

90