Allogenic Bioengineered Cartilage Achieves Hyaline Cartilage Repair in a Large Animal Model: A Promising Step Forward
The American Journal of Sports Medicine,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 27, 2025
Background:
Chondrocyte-based
cell
therapy
remains
a
promising
method
for
cartilage
repair,
despite
limitations
faced
during
the
last
30
years.
Purpose/Hypothesis:
This
work
presents
hyaline-like
bioengineered
beads
from
donor
chondrocytes
as
novel
treatment
option
lesions.
It
was
hypothesized
that
implanted
minigrafts
would
be
able
to
treat
lesions
by
complete
fusion
among
themselves
and
integration
with
surrounding
tissue.
No
tissue
rejection
expected
because
of
cartilage’s
reported
immunological
privilege.
Study
Design:
Controlled
laboratory
study.
Methods:
Allogenic
hyaline
characteristics
were
produced
frozen
minipig
donor.
A
total
8
Göttingen
minipigs
underwent
implantation
into
10
mm–diameter
full-thickness
chondral
(3
lesions/knee).
Animals
sacrificed
at
6
weeks
(n
=
2)
months
6)
after
implantation.
The
safety
efficacy
assessed
macroscopic
histological
analyses
well
magnetic
resonance
imaging.
Results:
signs
acute
or
chronic
observed
in
any
study
animals
upon
For
months,
imaging
results
showed
better
coverage
grafted
compared
empty
(control)
When
maintained
lesion,
subchondral
bone
native
observed.
Repair
quality
evidence
zonal
arrangement
months’
follow-up.
Additionally,
17)
had
repair
scores
than
7)
(mean
inverse
Goebel
score,
4.24
5.57,
respectively).
Graft-filled
only
slight
superiority
Bern
5.76
5.43,
Conclusion:
hold
potential
an
advanced
medicinal
product
treating
1-step
surgery
established
efficacy.
Clinical
Relevance:
successful
preclinical
highlights
allogenic
repair.
Moreover,
using
may
allow
reduced
patient
morbidity
surgery.
Hence,
this
is
suitable
large
older
patients
currently
being
evaluated
phase
I/IIa
clinical
trial.
Язык: Английский
Low‐density cultured cartilage cells expanded in platelet lysate present distinct features to develop an innovative clinical treatment for diffuse cartilage lesions
Knee Surgery Sports Traumatology Arthroscopy,
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 6, 2024
Abstract
Purpose
Chondrocyte‐based
cell
therapies
are
effective
for
the
treatment
of
chondral
lesions,
but
remain
poorly
indicated
diffuse
lesions
in
context
early
osteoarthritis
(OA).
The
aim
this
study
was
to
develop
a
protocol
obtain
chondroprogenitor
cells
suitable
within
OA.
Methods
Cartilage
were
expanded
at
low
density
human
platelet
lysate
(hPL).
A
test
performed
exclude
senescence.
expression
surface
cluster
differentiation
146,
166,
major
histocompatibility
complex
(MHC)‐I
and
MHC‐II
genes
interest
evaluated,
as
well
trophic
potential
these
cells,
by
assessment
lubricin
matrix
production.
immunomodulatory
assessed
through
their
co‐culture
with
macrophages.
Results
hPL
showed
higher
proliferation
rate
than
standard‐density
no
replicative
senescence,
immunogenicity
lubricin.
Moreover,
they
presented
an
increased
chondrogenic
antihypertrophic
markers,
superior
deposition
if
compared
cultured
standard
density.
induced
on
macrophages
upregulation
CD206,
although
increase
CD163
observed
presence
low‐density
cells.
Conclusions
These
findings
lay
grounds
explore
clinical
usefulness
cartilage
treat
OA
joints
both
autologous
allogenic
use.
Level
Evidence
Not
applicable.
Язык: Английский
Cell‐free biomimetic osteochondral scaffold for the treatment of knee articular surface lesions: Clinical outcomes differ based on patient and lesion characteristics
Knee Surgery Sports Traumatology Arthroscopy,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 5, 2024
Abstract
Purpose
A
cell‐free
biomimetic
osteochondral
scaffold
was
developed
to
treat
cartilage
knee
lesions,
with
positive
clinical
results
documented
in
small
case
series.
However,
clear
evidence
on
patient
and
lesion
characteristics
that
might
affect
the
outcome
is
still
lacking.
The
aim
of
this
study
analyse
a
large
cohort
patients
treated
investigate
factors
could
influence
outcome.
Methods
Two
hundred
three
(mean
age
30.7
±
10.9
years)
were
prospectively
evaluated
at
baseline,
6‐,
12‐
24‐month
follow‐up.
analysed
using
International
Knee
Documentation
Committee
(IKDC)
score,
activity
level
assessed
Tegner
score.
outcomes
analysed.
Results
Mild
severe
adverse
reactions
found
39.0%
1.5%
patients,
respectively.
failure
rate
2.0%,
increasing
12.3%
when
including
also
failures.
IKDC
subjective
score
increased
from
43.3
15.9
61.0
16.2
6
months,
68.3
18.5
12
months
73.8
18.3
24
(
p
<
0.0005).
improved
2.5
1.7
4.2
0.0005),
without
reaching
pre‐injury
(6.0
2.2)
objective
changed
68.5%
normal
nearly
knees
before
treatment
90.1%
months.
At
showed
correlation
ρ
=
−0.247;
women
had
lower
as
well
patellar
lesions
0.002).
Previous
surgery
correlated
0.003),
while
better
osteochondritis
dissecans
(OCD)
compared
degenerative
0.001).
Conclusion
This
safe
effective
for
offering
2
years
low
rate.
Better
observed
younger
femoral
condyles
OCD,
joints
affected
by
who
underwent
previous
surgery,
may
expect
results.
Level
Evidence
III,
Cohort
study.
Язык: Английский
Matrix‐assisted autologous chondrocyte transplantation is effective at mid/long‐term for knee lesions: A systematic review and meta‐analysis
Knee Surgery Sports Traumatology Arthroscopy,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 3, 2024
Язык: Английский
Osteoperiosteal Cylindrical Iliac Bone Graft and Minced Cartilage as an Osteochondral Autograft for a Large Osteochondral Lesion
Arthroscopy Techniques,
Год журнала:
2024,
Номер
13(12), С. 103135 - 103135
Опубликована: Июль 29, 2024
Mosaicplasty
is
a
relatively
challenging
procedure
used
in
the
management
of
focal
osteochondral
lesions
joints.
Donor-site
morbidity
still
main
concern
after
mosaicplasty
because
it
entails
harvesting
an
autograft
from
otherwise
healthy
region
to
be
impacted
later
on
weight-bearing
damaged
site.
We
describe
possible
alternative
conventional
with
subchondral
bone
support
harvested
iliac
crest
as
osteoperiosteal
and
covered
minced
cartilage
layer.
Язык: Английский
Adjuvante Injektionen nach operativer Knorpeltherapie
Knie Journal,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 21, 2024
Forty Years of the Use of Cells for Cartilage Regeneration: The Research Side
Pharmaceutics,
Год журнала:
2024,
Номер
16(12), С. 1622 - 1622
Опубликована: Дек. 22, 2024
Background:
The
treatment
of
articular
cartilage
damage
has
always
represented
a
problem
considerable
practical
interest
for
orthopedics.
Over
the
years,
many
surgical
techniques
have
been
proposed
to
induce
growth
repairing
tissue
and
limit
degeneration.
In
1994,
turning
point
occurred:
implanted
autologous
cells
paved
way
new
option
based
more
on
regeneration
than
repair.
Objectives:
This
review
aims
outline
biological
clinical
advances,
from
use
mature
adult
chondrocytes
cell-derived
products,
going
through
progenitor
derived
bone
marrow
or
adipose
their
concentrates
Moreover,
it
highlights
relevance
gene
therapy
as
valuable
tool
successfully
implementing
current
regenerative
treatments,
overcoming
limitations
local
delivery
factors.
Conclusions:
Finally,
this
concludes
with
an
outlook
importance
understanding
role
mechanisms
action
different
cell
compounds
view
personalized
treatments.
Язык: Английский