In
recent
years,
cancer
immunotherapy
has
undergone
a
transformative
shift
toward
personalized
and
targeted
therapeutic
strategies.
Bacteria-derived
outer
membrane
vesicles
(OMVs)
have
emerged
as
promising
adaptable
platform
for
due
to
their
unique
properties,
including
natural
immunogenicity
the
ability
be
engineered
specific
purposes.
this
review,
comprehensive
overview
is
provided
of
state-of-the-art
techniques
methodologies
employed
in
engineering
versatile
OMVs
immunotherapy.
Beginning
by
exploring
biogenesis
composition
OMVs,
unveiling
intrinsic
immunogenic
properties
appeal.
Subsequently,
innovative
approaches
engineer
are
delved
into,
ranging
from
genetic
parent
bacteria
incorporation
functional
molecules.
The
importance
rational
design
strategies
highlighted
enhance
specificity
allowing
tailoring
diverse
types.
Furthermore,
insights
into
clinical
studies
potential
challenges
utilizing
vaccines
or
adjuvants
also
provided,
offering
assessment
current
landscape
future
prospects.
Overall,
review
provides
valuable
researchers
involved
rapidly
evolving
field
immunotherapy,
roadmap
harnessing
full
treatment.
Nano Letters,
Год журнала:
2023,
Номер
23(17), С. 8115 - 8125
Опубликована: Авг. 29, 2023
Tumor-derived
extracellular
vesicles
(EVs)
are
promising
to
monitor
early
stage
cancer.
Unfortunately,
isolating
and
analyzing
EVs
from
a
patient's
liquid
biopsy
challenging.
For
this,
we
devised
an
EV
membrane
proteins
detection
system
(EV-MPDS)
based
on
Förster
resonance
energy
transfer
(FRET)
signals
between
aptamer
quantum
dots
AIEgen
dye,
which
eliminated
the
extraction
purification
conveniently
diagnose
lung
In
cohort
of
80
clinical
samples,
this
showed
enhanced
accuracy
(100%
versus
65%)
sensitivity
55%)
in
cancer
diagnosis
as
compared
ELISA
method.
Improved
screening
(from
96.4%
100%)
was
achieved
by
comprehensively
profiling
five
biomarkers
using
machine
learning
analysis
system.
FRET-based
tumor
EV-MPDS
is
thus
isolation-free,
low-volume
(1
μL),
highly
accurate
approach,
providing
potential
aid
screening.
Biomaterials Research,
Год журнала:
2023,
Номер
27(1)
Опубликована: Фев. 9, 2023
Extracellular
vesicles
(EVs)
are
nanosized
particles
that
released
from
cells
and
reflect
the
characteristics
of
mother
cell.
Recently,
EVs
have
been
used
in
several
types
studies
across
many
different
fields.
In
field
EV
research,
multiple
cell
culture
isolation
techniques
highlighted
importance.
Various
strategies,
including
exclusive
component
media,
three-dimensional
(3D)
cultures,
hypoxic
conditions,
proposed
for
to
control
function
EVs.
Ultracentrifugation,
ultrafiltration,
precipitation,
tangential
flow
filtration
(TFF)
utilized
isolation.
Although
isolated
their
own
functionalities,
researchers
trying
functionalize
by
applying
various
engineering
approaches.
Gene
editing,
exogenous,
endogenous,
hybridization
methods
four
well-known
functionalization
strategies.
engineered
through
these
processes
has
applied
regenerative
medicine,
kidney
diseases,
osteoarthritis,
rheumatoid
arthritis,
nervous
system-related
others.
this
review,
it
was
focused
on
approaches
applications
medicine.
Applied Materials Today,
Год журнала:
2024,
Номер
37, С. 102084 - 102084
Опубликована: Фев. 3, 2024
Extracellular
vesicles
(EVs)
are
biologically
active
nanoparticles
secreted
by
cells,
which
have
a
variety
of
physiological
functions,
such
as
promoting
cell
proliferation,
regulating
immunity,
anti-inflammation,
and
facilitating
angiogenesis.
However,
the
application
EVs
is
limited
defects
low
therapeutic
efficiency,
lack
targeting,
unstable
effects.
In
recent
years,
various
engineering
methods
been
developed
to
enhance
function
EVs.
addition,
research
on
isolation
mechanisms
has
also
made
significant
progress.
this
paper,
we
review
biogenesis,
composition,
techniques
EVs,
summarize
potential
natural
well
strategies
for
EV
modification,
highlight
advances
in
tissue
regeneration.
Finally,
discuss
EVs'
current
problems
future
perspectives
regenerative
medicine.
Pharmacological Research,
Год журнала:
2024,
Номер
204, С. 107202 - 107202
Опубликована: Май 3, 2024
Plant-derived
extracellular
vesicles
(PDEV)
constitute
nanoscale
entities
comprising
lipids,
proteins,
nucleic
acids
and
various
components
enveloped
by
the
lipid
bilayers
of
plant
cells.
These
play
a
crucial
role
in
facilitating
substance
information
transfer
not
only
between
cells
but
also
across
different
species.
Owing
to
its
safety,
stability,
abundance
raw
materials,
this
has
found
extensive
utilization
recent
years
within
research
endeavors
aimed
at
treating
diseases.
This
article
provides
an
overview
pathways
biological
characteristics
PDEV,
along
with
prevalent
methods
employed
for
isolation,
purification,
storage.
Furthermore,
we
comprehensively
outline
therapeutic
implications
diverse
sources
PDEV
musculoskeletal
system
disorders.
Additionally,
explore
as
platforms
engineering
drug
carriers,
aiming
delve
deeper
into
significance
potential
contributions
realm
system.
In
recent
years,
cancer
immunotherapy
has
undergone
a
transformative
shift
toward
personalized
and
targeted
therapeutic
strategies.
Bacteria-derived
outer
membrane
vesicles
(OMVs)
have
emerged
as
promising
adaptable
platform
for
due
to
their
unique
properties,
including
natural
immunogenicity
the
ability
be
engineered
specific
purposes.
this
review,
comprehensive
overview
is
provided
of
state-of-the-art
techniques
methodologies
employed
in
engineering
versatile
OMVs
immunotherapy.
Beginning
by
exploring
biogenesis
composition
OMVs,
unveiling
intrinsic
immunogenic
properties
appeal.
Subsequently,
innovative
approaches
engineer
are
delved
into,
ranging
from
genetic
parent
bacteria
incorporation
functional
molecules.
The
importance
rational
design
strategies
highlighted
enhance
specificity
allowing
tailoring
diverse
types.
Furthermore,
insights
into
clinical
studies
potential
challenges
utilizing
vaccines
or
adjuvants
also
provided,
offering
assessment
current
landscape
future
prospects.
Overall,
review
provides
valuable
researchers
involved
rapidly
evolving
field
immunotherapy,
roadmap
harnessing
full
treatment.
