Extracellular vesicles in skin health, diseases, and aging

Deleted Journal, Год журнала: 2024, Номер 2(3)

Опубликована: Май 29, 2024

Abstract The skin interfaces with the external environment, acting as both a physical barrier and an immunologic defense. dynamic interactions between various cell types are essential for homeostasis function. Emerging research has unveiled significant role of extracellular vesicles (EVs) key mediators communication in regulating many aspects physiological pathological processes. In this review, we provide overview recent advances understanding roles EVs health, aging, diseases, discuss natural engineered disease treatment along potential challenges. burgeoning area may expand new therapeutic approaches disorders open avenues personalized skincare treatments.

Язык: Английский

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Extracellular vesicle encapsulated nicotinamide delivered via a trans-scleral route provides retinal ganglion cell neuroprotection

Acta Neuropathologica Communications, Год журнала: 2024, Номер 12(1)

Опубликована: Апрель 22, 2024

The progressive and irreversible degeneration of retinal ganglion cells (RGCs) their axons is the major characteristic glaucoma, a leading cause blindness worldwide. Nicotinamide adenine dinucleotide (NAD) cofactor metabolite redox reaction critical for neuronal survival. Supplementation with nicotinamide (NAM), precursor NAD, can confer neuroprotective effects against glaucomatous damage caused by an age-related decline NAD or mitochondrial dysfunction, reflecting high metabolic activity RGCs. However, oral supplementation drug relatively less efficient in terms transmissibility to RGCs compared direct delivery methods such as intraocular injection using subconjunctival depots. Neither method ideal, given risks infection scarring without novel techniques. By contrast, extracellular vesicles (EVs) have advantages system low immunogeneity tissue interactions. We evaluated EV NAM RGC protective agent quantitative assessment dendritic integrity DiOlistics, which confirmed be more sensitive measure health our mouse glaucoma model than evaluation somatic loss via immunostaining method. NAM-loaded EVs showed significant effect explant model. Furthermore, penetrate sclera once deployed space. These results confirm feasibility deliver targets.

Язык: Английский

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Control of articular degeneration by extracellular vesicles from stem/stromal cells as a potential strategy for the treatment of osteoarthritis

Biochemical Pharmacology, Год журнала: 2024, Номер 228, С. 116226 - 116226

Опубликована: Апрель 23, 2024

Osteoarthritis (OA) is a degenerative joint condition that contributes to years lived with disability. Current therapeutic approaches are limited as there no disease-modifying interventions able delay or inhibit the progression of disease. In recent has been an increasing interest in immunomodulatory and regenerative properties mesenchymal stem/stromal cells (MSCs) develop new OA therapies. Extracellular vesicles (EVs) mediate many biological effects these may represent alternative avoiding limitations cell-based therapy. There also growing EV modifications enhance their efficacy applications. Recent preclinical studies have provided strong evidence supporting potential MSC EVs for development treatments. Thus, regulate chondrocyte functions avoid cartilage destruction, abnormal subchondral bone metabolism synovial tissue alterations, control pain behavior. actions be mediated by transfer cargo target cells, important role proteins non-coding RNAs modulating signaling pathways relevant progression. Nevertheless, additional investigations needed concerning optimization, standardization preparation procedures. More research required better knowledge possible on different phenotypes, pharmacokinetics, mechanism action, long-term safety profile. Furthermore, high vehicles drug delivery adjuvant therapy potentiate complement other approaches.

Язык: Английский

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Multiplexed PLGA scaffolds with nitric oxide-releasing zinc oxide and melatonin-modulated extracellular vesicles for severe chronic kidney disease

Journal of Advanced Research, Год журнала: 2024, Номер 69, С. 75 - 89

Опубликована: Март 25, 2024

With prevalence of chronic kidney disease (CKD) in worldwide, the strategies to recover renal function via tissue regeneration could provide alternatives replacement therapies. However, due relatively low reproducibility basal cells and limited bioactivities implanted biomaterials along with high probability substance-inducible inflammation immunogenicity, be challenging. To exclude various side effects from cell transplantations, this study, we have induced extracellular vesicles (EVs) incorporated cell-free hybrid PMEZ scaffolds. Hybrid scaffolds incorporating essential bioactive components, such as ricinoleic acid grafted Mg(OH)2 (M), matrix (E), alpha lipoic acid-conjugated ZnO (Z) based on biodegradable porous PLGA (P) platform was successfully manufactured. Consecutively, for functional improvements, melatonin-modulated (mEVs), derived human umbilical cord MSCs chemically defined media without serum impurities, were also loaded onto construct multiplexed PMEZ/mEV scaffold. functionalities matrix-loaded scaffolds, continuous nitric oxide-releasing property modified remarkably upregulated regenerative mEVs showed significantly enhanced activities. Based these, structural restoration has been practically achieved 5/6 nephrectomy mouse models that mimicked severe CKD. Our study proved combinatory PLGA-based scaffold representing a CKD model. The optimal microenvironments morphogenetic formations tissues restorations remarkable components PMEZ/mEV, which promising therapeutic alternative treatment.

Язык: Английский

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Extracellular Vesicle microRNA: A Promising Biomarker and Therapeutic Target for Respiratory Diseases

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9147 - 9147

Опубликована: Авг. 23, 2024

Respiratory diseases, including chronic obstructive pulmonary disease (COPD), asthma, lung cancer, and coronavirus pneumonia, present a major global health challenge. Current diagnostic therapeutic options for these diseases are limited, necessitating the urgent development of novel biomarkers strategies. In recent years, microRNAs (miRNAs) within extracellular vesicles (EVs) have received considerable attention due to their crucial role in intercellular communication progression. EVs membrane-bound structures released by cells into environment, encapsulating variety biomolecules such as DNA, RNA, lipids, proteins. Specifically, miRNAs EVs, known EV-miRNAs, facilitate regulating gene expression. The expression levels can reflect distinct states significantly influence immune cell function, airway inflammation, remodeling, proliferation, angiogenesis, epithelial-mesenchymal transition, other pathological processes. Consequently, EV-miRNAs profound impact on onset, progression, responses respiratory with great potential management. Synthesizing current understanding COPD, this review aims explore targets examine prospects diagnosis treatment diseases.

Язык: Английский

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Leveraging engineered yeast small extracellular vesicles serve as multifunctional platforms for effectively loading methyl salicylate through the “esterase-responsive active loading” strategy

European Journal of Pharmaceutics and Biopharmaceutics, Год журнала: 2025, Номер unknown, С. 114696 - 114696

Опубликована: Март 1, 2025

Язык: Английский

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Mechanisms Tackling Salivary Gland Diseases with Extracellular Vesicle Therapies

Journal of Dental Research, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Extracellular vesicles (EVs) are lipid-enclosed particles released from cells, containing lipids, DNA, RNA, metabolites, and cytosolic cell surface proteins. EVs support intercellular communication orchestrate organogenesis by transferring bioactive molecules in between cells. Mesenchymal stem cells known to produce EVs, which exhibit immunomodulatory regenerative capabilities many target organs, including the salivary glands (SGs). Since cell-based therapies still pose challenges (e.g., donor variability, limited hemocompatibility, safety), specific may constitute a therapeutic alternative for SG diseases. New EV guidelines (MISEV2023) have recently been updated reported our consortium consolidate principles of biology expand boundaries toward innovative therapies. These provide valuable guidance researchers consistently assess effectiveness mesenchymal cell–derived cargo cues, such as microRNA, proteins, other molecules, This review provides narrative synthesis preclinical studies on highlighting mechanisms their potential applications diseases, radiotherapy-induced hypofunction Sjögren’s syndrome, well inflammatory aging-related conditions. Additionally, we highlight key areas MISEV2023 that will future EV-based research. adhered PRESS (Peer Review Electronic Search Strategies) utilized established databases, Medline/PubMed, Embase, Web Science, Scopus, alongside machine learning tools sorting most impactful

Язык: Английский

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Advances in Plant-Derived Extracellular Vesicle Extraction Methods and Pharmacological Effects

Biology, Год журнала: 2025, Номер 14(4), С. 377 - 377

Опубликована: Апрель 6, 2025

Extracellular vesicles (EVs) are those with a double-membrane structure that contains proteins, lipids, nucleic acids, and other biologically active substances play an important role in cell–cell cell–environment communication. They have also become mechanism for exchanging cellular molecules. As many studies on EVs been conducted, plant-derived extracellular (PDEVs) started attracting attention. The biological activity stability of PDEVs closely related to the extraction separation methods, choosing method meets requirements is important. methods include ultracentrifugation, ultrafiltration, size-exclusion chromatography, etc. In recent years, it has found through research possess properties, such as anti-inflammatory, anti-cancer, anti-infective they show unique advantages therapeutic agents drug carriers. Therefore, we collected scientific literature derived from more than dozen fruits vegetables, summarized analyzed their extraction, separation, roles disease treatment, aiming provide reference inspiration in-depth study efficacy new drugs.

Язык: Английский

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Extracellular Vesicles as Drug Delivery System for Cancer Therapy

Pharmaceutics, Год журнала: 2024, Номер 16(8), С. 1029 - 1029

Опубликована: Авг. 1, 2024

In recent decades, the pursuit of drug delivery systems has led to development numerous synthetic options aimed at enhancing efficacy while minimizing side effects. However, practical application these is often hindered by challenges such as inefficiency, cytotoxicity, and immunogenicity. Extracellular vesicles, natural carriers for drugs, emerge promising alternatives with distinct advantages over carriers. Notably, EVs exhibit biocompatibility, low immunogenicity, inherent tissue-targeting capabilities, thus opening new avenues strategies. This review provides an overview EVs, including their biogenesis absorption mechanisms. Additionally, we explore current research efforts focusing on harnessing potential carriers, encompassing aspects purification techniques, loading, bioengineering targeted delivery. Finally, discuss existing future prospects therapeutic agents in clinical settings. comprehensive analysis aims shed light versatile effective tools delivery, particularly realm cancer therapy.

Язык: Английский

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Bidirectional modulation of extracellular vesicle-autophagy axis in acute lung injury: Molecular mechanisms and therapeutic implications

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 180, С. 117566 - 117566

Опубликована: Окт. 17, 2024

Язык: Английский

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