Annals of Medicine,
Год журнала:
2025,
Номер
57(1)
Опубликована: Апрель 18, 2025
Extracellular
vesicles
(EVs)
are
promising
biomarkers
for
neurodegeneration.
Alu
elements
retrotransposons
increasingly
expressed
with
age
and
may
be
involved
in
aging-related
diseases.
To
determine
the
potential
of
RNA
plasma-derived
EVs
as
a
biomarker
brain
aging
neuronal
injury.
were
isolated
from
plasma
samples
across
different
groups.
EV
levels
measured
their
associations
aging,
including
neurofilament
light
chain
(NfL),
amyloid-beta
(Aβ42
Aβ40),
phosphorylated
tau
(p-Tau181),
analyzed.
increased
significantly
strongly
correlated
NfL,
suggesting
strong
association
between
Significant
correlations
also
found
levels,
while
no
significant
was
observed
pathology.
elevated
associated
injury,
highlighting
novel,
non-invasive
Journal of Extracellular Biology,
Год журнала:
2025,
Номер
4(1)
Опубликована: Янв. 1, 2025
Abstract
Current
state‐of‐the‐art
tools
for
analysing
extracellular
vesicles
(EVs)
offer
either
highly
sensitive
but
unidimensional
bulk
measurements
of
EV
components,
or
high‐resolution
multiparametric
single‐particle
analyses
which
lack
standardization
and
appropriate
reference
materials.
This
limits
the
accuracy
assessment
marker
abundance
overall
distribution
amongst
individual
EVs,
finally,
understanding
true
heterogeneity.
In
this
study,
we
aimed
to
define
standardized
operating
procedures
material
fluorescent
characterization
EVs
with
two
commonly
used
analytical
platforms—nanoparticle
tracking
analysis
(NTA)
nano‐flow
cytometry
(nFCM).
We
achieved
quantitative
fluorescence
on
ZetaView
NTA
NanoAnalyzer
nFCM
instruments,
by
utilizing
yellow‐green
FluoSpheres
(FS)
assigned
ERF
(equivalent
fluorophore)
values.
technique
allowed
signal
be
expressed
in
units
(indicative
bound
antibodies
per
EV),
thus
enabling
measurement
target
protein
whole
population.
The
NTA's
nFCM's
detection
(LoD)
were
evaluated
at
21
9
Alexa
Fluor
488
(AF488)
molecules,
respectively.
To
complement
limited
quantification
markers
a
few
copies
single
EV,
in‐line
plate
reader
performed.
provided
absolute
more
insightful
heterogeneity
stoichiometry.
method
outlined
work
unlocks
full
potential
nFCM,
cross‐platform
data
comparison.
At
same
time,
it
highlights
some
technical
challenges
considerations
contributes
ongoing
efforts
towards
development
tools.
Seminars in Cancer Biology,
Год журнала:
2024,
Номер
101, С. 12 - 24
Опубликована: Апрель 23, 2024
In
2014,
the
International
Society
for
Extracellular
Vesicles
(ISEV)
introduced
Minimal
Information
Studies
of
(MISEV)
guidelines
to
establish
standards
extracellular
vesicle
(EV)
research.
These
aimed
enhance
reliability
and
reproducibility,
addressing
expanding
field
EV
science.
EVs,
membrane-bound
particles
released
by
cells,
play
crucial
roles
in
intercellular
communication
are
potential
biomarkers
various
conditions.
Over
years,
landscape
witnessed
a
surge
publications,
emphasizing
their
cancer
immune
modulation.
response,
MISEV
underwent
evolution,
leading
MISEV2018
update.
This
version,
generated
through
community
outreach,
provided
comprehensive
framework
research
methodologies,
separation,
characterization,
reporting
standards,
engagement.
The
reflected
responsiveness
feedback,
acknowledging
evolving
landscape.
served
as
testament
commitment
scientific
rigorous
collective
discernment
experts.
present
article
compares
previous
with
its
2023
counterpart,
highlighting
advancements,
changes,
impacts
on
standardization.
build
upon
2018
principles,
offering
new
recommendations
emerging
areas.
comparative
exploration
contributes
understanding
transformative
journey
research,
MISEV's
pivotal
role
community's
adaptability
challenges.
Abstract
Small
non‐coding
RNAs
(ncRNAs)
are
functional
molecules
contained
within
extracellular
vesicles
(EVs)
that
modulate
various
physiological
and
pathological
processes.
This
study
provides
a
comprehensive
expression
profile
of
seven
types
small
ncRNAs
in
serum‐
plasma‐derived
EVs
under
conditions.
Both
large
(lEVs)
(sEVs)
contain
high
proportions
miRNAs
(∼28.2%
lEVs
∼20.8%
sEVs)
ribosomal
(∼24.0%
∼19.1%
sEVs).
enriched
with
more
transfer
RNA
(∼38.8%)
than
sEVs,
whereas
sEVs
have
greater
abundance
Y
(∼22.5%).
Notably,
is
abundant
obtained
from
aged
samples
(age
≥60
years),
pattern
not
observed
lEVs.
diverse
serum‐derived
EVs.
There
also
degree
overlap
(>50%)
the
top
100
identified
sEVs.
The
hsa‐miR‐16‐5p,
hsa‐let‐7a‐5p,
hsa‐miR‐142‐3p,
hsa‐miR‐103‐3p
consistently
among
10
highly
expressed
plasma‐
as
well
peripheral
blood
mononuclear
cells.
Serum‐derived
glioblastoma,
breast
cancer,
prostate
gastric
cancer
specific,
miRNAs,
snoRNAs,
nuclear
RNAs,
piRNAs.
These
results
elucidate
patterns
ncRNA
cargoes
derived
serum
plasma
conditions
offer
valuable
insights
for
future
diagnostic
therapeutic
applications.
Extracellular
vesicles
(EVs)
are
released
by
all
kind
of
cells
into
the
extracellular
space,
where
they
shuttle
parental
cell‐derived
molecular
cargoes
(DNA,
RNA,
proteins)
to
both
adjacent
and
distant
cells,
influencing
physiology
target
cells.
Their
specific
cargo
content
abundance
in
liquid
biopsies
make
them
excellent
candidates
for
biomarker
studies.
Indeed,
EVs
isolated
from
various
body
fluids,
including
blood,
pleural
fluid,
urine,
cerebrospinal
saliva,
milk,
ascites,
tears,
have
been
recognized
their
potential
as
biomarkers
diagnosis,
monitoring
treatment,
predicting
outcomes
diseases.
Increasing
studies
suggest
that
tears
great
promise
a
noninvasive
biopsy
source
EVs.
Our
aim
here
is
provide
comprehensive
review
exploration
reservoir
contents,
evaluating
accessibility
utility
method.
Additionally,
tear
cancers,
ocular
cancer,
discussed.
Finally,
advantages
challenges
employing
tear‐based
disease
'
s
evaluated.
Journal of Extracellular Biology,
Год журнала:
2025,
Номер
4(1)
Опубликована: Янв. 1, 2025
The
extracellular
vesicle
release
in
red
blood
cell
concentrates
reflects
progressive
accumulation
of
storage
lesions
and
could
represent
a
new
measure
to
be
implemented
routinely
centres
addition
haemolysis.
Nevertheless,
there
is
currently
no
standardized
isolation
protocol.
In
previous
publication,
we
developed
reproducible
ultracentrifugation-based
protocol
(20,000
×
g
protocol)
that
allows
classify
into
three
cohorts
according
their
vesiculation
level.
Since
this
was
not
adapted
meet
routine
requirements,
the
goal
study
develop
an
easier
method
based
on
low-speed
centrifugation
(2,000
limited
concentrate
volumes
match
with
non-destructive
sampling
from
quality
control
tubing.
Despite
presence
contaminants,
mainly
form
albumin
lipoproteins,
material
isolated
2,000
contained
cell-derived
vesicular
structures.
It
reproducible,
predict
number
vesicles
obtained
20,000
better
discriminated
between
than
haemolysis
at
legal
expiry
date
6
weeks.
However,
by
decreasing
fit
volume
tubing,
particle
yield
highly
reduced.
Therefore,
time
relative
centrifugal
force
were
(1,000
protocol),
allowing
for
recovery
similar
composition
small
large
sampled
main
unit,
different
over
time.
A
observation
made
1,000
tubing
mother-bag.
conclusion,
our
paves
way
use
(adapted
tubing)
measurement
centres.
Journal of Extracellular Vesicles,
Год журнала:
2025,
Номер
14(1)
Опубликована: Янв. 1, 2025
ABSTRACT
Despite
immense
interest
in
biomarker
applications
of
extracellular
vesicles
(EVs)
from
blood,
our
understanding
circulating
EVs
under
physiological
conditions
healthy
humans
remains
limited.
Using
imaging
and
multiplex
bead‐based
flow
cytometry,
we
comprehensively
quantified
with
respect
to
their
cellular
origin
a
large
cohort
blood
donors.
We
assessed
coefficients
variations
characterize
biological
variation
explored
demographic,
clinical,
lifestyle
factors
contributing
observed
variation.
Cell‐specific
EV
subsets
show
wide
range
concentrations
that
do
not
correlate
cell‐of‐origin
suggesting
steady‐state
subset
are
regulated
by
complex
mechanisms,
which
differ
even
for
the
same
cell
type.
Interestingly,
tetraspanin+
largely
originate
platelets
lesser
extent
lymphocytes.
Principal
component
analysis
(PCA)
association
analyses
demonstrate
high
inter‐individual
across
humans,
only
partly
explained
influence
sex,
menopausal
status,
age
smoking
on
specific
and/or
subsets.
No
global
subject's
was
detected.
Our
findings
provide
first
comprehensive,
quantitative
data
towards
cell‐origin
atlas
plasma
EVs,
important
implications
clinical
use
as
biomarkers.
