Plasma extracellular vesicle: a novel biomarker for neurodegenerative disease diagnosis DOI Open Access
Xinrui Zhao,

Shenglin Huang

Extracellular Vesicles and Circulating Nucleic Acids, Год журнала: 2024, Номер 5(3), С. 669 - 73

Опубликована: Сен. 30, 2024

Extracellular vesicles (EVs) are membrane-bound structures that carry proteins, lipids, RNA, and DNA, playing key roles in cell communication material transport. Recent research highlights their potential as disease biomarkers due to stability bodily fluids. This study explores using tau TDP-43 proteins plasma EVs diagnostic for frontotemporal dementia (FTD) amyotrophic lateral sclerosis (ALS). Analyzing from clinical cohorts, the found 3R/4R ratio levels effectively differentiate between groups with high accuracy. Notably, EV demonstrate higher accuracy compared direct testing, providing new insights approaches future practice. Further is needed validate these diverse populations establish standardized protocols. Future studies should continue explore of a broader range neurodegenerative diseases delve deeper into mechanisms secretion sorting enhance utility.

Язык: Английский

Single‐extracellular vesicle (EV) analyses validate the use of L1 Cell Adhesion Molecule (L1CAM) as a reliable biomarker of neuron‐derived EVs DOI Creative Commons
Carlos Nogueras‐Ortiz, Erden Eren, Pamela J. Yao

и другие.

Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(6)

Опубликована: Июнь 1, 2024

Abstract Isolation of neuron‐derived extracellular vesicles (NDEVs) with L1 Cell Adhesion Molecule (L1CAM)‐specific antibodies has been widely used to identify blood biomarkers CNS disorders. However, full methodological validation requires demonstration L1CAM in individual NDEVs and lower levels or absence EVs from other cells. Here, we multiple single‐EV techniques establish the neuronal origin determine abundance L1CAM‐positive human blood. epitopes ectodomain are shown be co‐expressed on single‐EVs proteins β‐III‐tubulin, GAP43, VAMP2, which increase parallel enrichment EVs. Levels carrying VAMP2 β‐III‐tubulin range 30% 63%, contrast 0.8%–3.9% L1CAM‐negative Plasma fluid‐phase does not bind single‐EVs. Our findings support use as a target for isolating plasma leveraging their cargo reflecting function.

Язык: Английский

Процитировано

15
Neuron‐derived extracellular vesicles as a liquid biopsy for brain insulin dysregulation in Alzheimer's disease and related disorders DOI Creative Commons

Jacob Cleary,

Ashish Kumar, Suzanne Craft

и другие.

Alzheimer s & Dementia, Год журнала: 2025, Номер unknown

Опубликована: Янв. 17, 2025

Extracellular vesicles (EVs) have emerged as novel blood-based biomarkers for various pathologies. The development of methods to enrich cell-specific EVs from biofluids has enabled us monitor difficult-to-access organs, such the brain, in real time without disrupting their function, thus serving liquid biopsy. Burgeoning evidence indicates that contents neuron-derived (NDEs) blood reveal dynamic alterations occur during neurodegenerative pathogenesis, including Alzheimer's disease (AD), reflecting a disease-specific molecular signature. Among these AD-specific changes is brain insulin-signaling dysregulation, which cannot be assessed clinically living patient and remains an unexplained co-occurrence AD pathogenesis. This review focused on delineating how NDEs may begin close gap between identifying associated with insulin dysregulation reliably patients its connection AD. approach could lead identification early less-invasive diagnostic HIGHLIGHTS: Neuron-derived extracellular isolated peripheral blood. reflect signature (AD). Brain plays critical role predict dysregulation. offer

Язык: Английский

Процитировано

1
Extracellular vesicles: translational research and applications in neurology DOI
Apostolos Manolopoulos, Pamela J. Yao, Dimitrios Kapogiannis

и другие.

Nature Reviews Neurology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

Язык: Английский

Процитировано

1
Extracellular Vesicle-Mediated Neuron–Glia Communications in the Central Nervous System DOI
Tsuneya Ikezu, Yongjie Yang, Claudia Verderio

и другие.

Journal of Neuroscience, Год журнала: 2024, Номер 44(40), С. e1170242024 - e1170242024

Опубликована: Окт. 2, 2024

Communication between neurons and glia significantly influences the development maturation, plasticity, disease progressions of nervous system. As a new signaling modality, extracellular vesicles display diverse role for robust functional regulation through their protein nucleic acid cargoes. This review highlights recent breakthroughs in research mechanisms mediated by that are important neural development, axonal maintenance, synaptic functions, progression mammalian We will discuss biological roles released from neurons, astroglia, microglia, oligodendroglia system implications neurodegenerative disorders.

Язык: Английский

Процитировано

5
Single extracellular vesicle research: From cell population to a single cell DOI
Ting Ting Wang, Wanqiu Huang, Xu Gao

и другие.

Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 734, С. 150439 - 150439

Опубликована: Июль 23, 2024

Язык: Английский

Процитировано

3
Extracellular vesicles: new horizons in neurodegeneration DOI Creative Commons
Jun Chen, Chen Tian, Xiong Xiao

и другие.

EBioMedicine, Год журнала: 2025, Номер 113, С. 105605 - 105605

Опубликована: Март 1, 2025

Extracellular vesicles (EVs) are lipid-enclosed nanovesicles secreted by diverse cell types that orchestrate intercellular communication through cargo delivery. Their pivotal roles span from supporting the development of normal central nervous system (CNS) to contributing pathogenesis neurological diseases. Particularly noteworthy is their involvement in propagation pathogenic proteins, such as those involved neurodegenerative disorders, and nucleic acids, closely linking them disease onset progression. Moreover, EVs have emerged promising diagnostic biomarkers for disorders tools staging, owing ability traverse blood-brain barrier specific, stable, accessible properties. This review comprehensively explores realm CNS-derived found peripheral blood, encompassing detection methods, transport mechanisms, various Furthermore, we evaluate potentials limitations clinical applications highlight prospective research directions this rapidly evolving field.

Язык: Английский

Процитировано

0
Differential miRNA expression in neural-enriched extracellular vesicles as potential biomarker for frontotemporal dementia and bipolar disorder DOI Creative Commons
María Serpente, Giuseppe Delvecchio, Chiara Fenoglio

и другие.

Neurobiology of Disease, Год журнала: 2025, Номер unknown, С. 106867 - 106867

Опубликована: Март 1, 2025

Behavioral variant of Frontotemporal Dementia (bvFTD) and Bipolar Disorder (BD) share overlapping symptoms, complicating diagnosis. BvFTD, especially linked to C9orf72 expansions, often mimics BD, highlighting the need for reliable biomarkers. This study aimed differentiate bvFTD from BD using miRNA profiles in neural-enriched extracellular vesicles (NEVs). A cohort 100 subjects was analyzed: 40 (20 sporadic, 20 carriers), healthy controls. NEVs were isolated plasma profiled real-time PCR. Among 754 miRNAs, 11 significantly deregulated BD. MiR-152-5p downregulated sporadic bvFTD, while let-7b, let-7e, miR-18b, miR-142-5p altered carriers. patients showed distinct patterns miR-331-5p, miR-335, miR-345 compared bvFTD. Bioinformatics analyses revealed that common long non-coding RNA (lncRNA) targets, including XIST, NEAT1, OIP5-AS1, suggesting their involvement molecular networks relevant C9orf72-related These signatures can cases, offer insights into disease pathways. Further research is needed validate these findings explore clinical application.

Язык: Английский

Процитировано

0
Extracellular vesicles in mood disorders: A systematic review of human studies DOI Creative Commons
Cristian-Daniel Llach, Gia Han Le, Sebastian Badulescu

и другие.

European Neuropsychopharmacology, Год журнала: 2025, Номер 94, С. 59 - 75

Опубликована: Март 9, 2025

Extracellular vesicles (EVs) are small, membrane-bound particles that naturally released by nearly all cell types in the body. They serve as molecular biosignatures, reflecting state of their cells origin and providing a non-invasive peripheral marker central nervous system (CNS) activity under physiological pathological conditions. We conducted systematic review (ID: CRD42024528824) studies investigating use EVs mood disorders within clinical populations. screened articles indexed PubMed, EMBASE, Scopus, ISI Web Science, APA PsycInfo from January 2010 to October 2024. Available research has focused on four key areas: (1) EV cargo mechanistic diagnostic biomarkers; (2) predictive or tracking biomarkers for antidepressant response; (3) neuroimaging correlates; (4) physical properties. Most examined major depressive disorder (MDD), with others addressing bipolar (BD), adolescent depression, postpartum late-life depression. Notably, only 35,55 % utilized brain-derived EVs. Through analyses EV-derived miRNA, proteins, mtDNA, metabolites, these have explored neural mitochondrial function, brain insulin resistance, neurogenesis, neuroinflammation, blood-brain barrier permeability context disorders. Some markers demonstrated potential. This discusses findings, limitations current research, future directions leveraging study

Язык: Английский

Процитировано

0
Most L1CAM Is not Associated with Extracellular Vesicles in Human Biofluids and iPSC–Derived Neurons DOI Creative Commons

Vaibhavi Kadam,

Madeleine Wacker,

Patrick Oeckl

и другие.

Molecular Neurobiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

Abstract Transmembrane L1 cell adhesion molecule (L1CAM) is widely used as a marker to enrich for neuron-derived extracellular vesicles (EVs), especially in plasma. However, this approach lacks sufficient robust validation. This study aimed assess whether human biofluids are indeed enriched EVs, particularly by L1CAM immunoaffinity, utilizing multiple sources (plasma, CSF, conditioned media from iPSC-derived neurons [iNCM]) and different methods (mass spectrometry [MS], nanoparticle tracking analysis [NTA]). Following systematic multi-step validation approach, we confirmed isolation of generic EV populations using size-exclusion chromatography (SEC) polymer-aided precipitation (PPT)—two most commonly applied methods—from all sources. Neurofilament light (NfL) was detected both CSF blood-derived indicating their neuronal origin. immunoprecipitation did not yield enrichment fractions. Instead, it predominantly found its free-floating form. Additionally, MS-based proteomic CSF-derived EVs also show enrichment. Our validates diverse biofluid several approaches confirms that some subpopulations Thorough testing across orthogonal methods, however, does support reliably specific subpopulation

Язык: Английский

Процитировано

0
Noninvasive Assessment of β‐Secretase Activity Through Click Chemistry‐Mediated Enrichment of Neuronal Extracellular Vesicles to Detect Alzheimer's Disease DOI Creative Commons
Hyo Yong Kim, Junseok Lee,

Audrey Qian

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

Abstract Alzheimer's disease (AD), the most prevalent type of dementia, is characterized by a biological process that begins with development AD neuropathologic change (ADNPC) while individuals remain asymptomatic. A key molecular hallmark ADNPC accumulation amyloid‐β plaques. β‐secretase plays critical role in upstream pathological cleavage amyloid precursor protein (APP), producing peptides are prone to misfolding, ultimately contributing plaque formation. Neuronal extracellular vesicles (NEVs) blood transport and preserve its activity, allowing for noninvasive profiling activity detecting early onset ADNPC. In this study, novel approach approached assessment patients using an NEV assay. This assay identifies NEVs exhibiting colocalization markers AD‐associated β‐secretase, generating profile each patient. The represents significant advancement leveraging diagnostic potential NEVs, offering noninvasive, quantitative method reliably assessing detect

Язык: Английский

Процитировано

0