Quantification of urinary podocyte‐derived migrasomes for the diagnosis of kidney disease

Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(6)

Опубликована: Июнь 1, 2024

Abstract Migrasomes represent a recently uncovered category of extracellular microvesicles, spanning diameter range 500 to 3000 nm. They are emitted by migrating cells and harbour diverse array RNAs proteins. can be readily identified in bodily fluids like serum urine, rendering them valuable non‐invasive source for disease diagnosis through liquid biopsy. In this investigation, we introduce streamlined effective approach the capture quantitative assessment migrasomes, employing wheat germ agglutinin (WGA)‐coated magnetic beads flow cytometry (referred as WBFC). Subsequently, examined levels migrasomes urine kidney (KD) patients with podocyte injury healthy volunteers using WBFC. The outcomes unveiled substantial increase urinary podocyte‐derived migrasome concentrations among individuals KD compared counterparts. Notably, were found express an abundant quantity phospholipase A2 receptor (PLA2R) presence PLA2R proteins these holds promise serving natural antigen quantification autoantibodies against afflicted membranous nephropathy. Consequently, our study not only pioneers novel technique isolation but also underscores potential promising biomarker early injury.

Язык: Английский

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Autologous platelet delivery of siRNAs by autologous plasma protein self-assembled nanoparticles for the treatment of acute kidney injury

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Март 29, 2025

Acute kidney injury (AKI) involves the activation of intrarenal hemostatic and inflammatory pathways. Platelets rapidly migrate to affected sites AKI release extracellular vesicles (EVs) laden with bioactive mediators that regulate inflammation hemostasis. While small interfering RNA (siRNA) is a potent gene-silencing tool for biomedical applications, its therapeutic application in vivo remains challenging. We developed an innovative nucleic acid delivery platform by hybridizing synthetic transformation-related protein 53 (p53) siRNA autologous plasma incubating complex platelets. These engineered platelets selectively delivered p53 injured renal tubular cells via EV-mediated cargo release, resulting targeted suppression subsequent attenuation progression. This platelet-centric translational strategy demonstrates significant potential advancing precision therapies exploiting endogenous platelet trafficking deliver therapeutics directly sites.

Язык: Английский

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Migrasomes, critical players in intercellular communication

Cancer Cell International, Год журнала: 2025, Номер 25(1)

Опубликована: Март 25, 2025

Migrasomes are a newly discovered type of extracellular vesicle (EV) formed during cell migration, playing pivotal role in intercellular communication. These vesicles generated by retracting fibers migrating cells and encapsulate various molecules, such as proteins, lipids, RNA, allowing the transfer biochemical signals to neighboring cells. Current evidence suggests that migrasomes involved wide range physiological processes embryogenesis, angiogenesis, immune modulation, mitochondrial quality control. Moreover, implicated pathological conditions, including cancer metastasis, cardiovascular diseases, viral infections. To fully understand their significance, it is critical first explore molecular mechanisms underlying formation function. Recent studies have shed light on biogenesis, release, biological properties migrasomes, all which key understanding cell-to-cell In this review, we provide an up-to-date summary migrasome characterization, activities communication, while also proposing potential new functions for these vesicles.

Язык: Английский

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A panel of miRNAs in the serum extracellular vesicles serve as novel diagnostic biomarkers for MASLD

Biomedical Journal, Год журнала: 2025, Номер unknown, С. 100838 - 100838

Опубликована: Фев. 1, 2025

The increased prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) and its profound implications for global health have sparked extensive research endeavors aimed at developing potential diagnostic methods this condition. Despite the achievements in defining various environmental factors genetic predispositions linked to MASLD, diagnosis clinical staging remain challenging. Recently, extracellular vesicles (EVs) garnered considerable attention owing their roles dysfunctions as biomarkers conditions. This study investigate whether microRNAs (miRNAs) serum EVs could be utilized diagnosing MASLD. We applied an innovative efficient approach that involves capturing analyzing using wheat germ agglutinin (WGA)-coupled magnetic beads, subsequently employing reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis. MiR-574-3p, miR-542-3p, miR-200a-3p were significantly elevated patients with indicating markers. has established a straightforward assay platform isolating without need purification quantitatively detecting miR-574-3p, vesicles.

Язык: Английский

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Migrasome-related ITGA5 for predicting prognosis, immune infiltration and drug sensitivity of hepatocellular carcinoma

APOPTOSIS, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

Язык: Английский

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Migrasomes: Critical players in intercellular nanovesicle communication

Cellular Signalling, Год журнала: 2025, Номер unknown, С. 111796 - 111796

Опубликована: Апрель 1, 2025

Язык: Английский

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Migrasomes as intercellular messengers: potential in the pathological mechanism, diagnosis and treatment of clinical diseases

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 20, 2025

Migrasomes are newly identified organelles that were first discovered in 2015. Since then, their biological structure, formation process, and physiological functions have been gradually elucidated. Research recent years has expanded our understanding of these aspects, highlighting significance various pathological processes. found to play crucial roles normal functions, including embryonic development, vascular homeostasis, material transport, mitochondrial quality control. Additionally, emerging evidence suggests involvement diseases; however, clinical research on remains limited. Current studies indicate migrasomes may contribute disease pathogenesis hold potential for diagnostic therapeutic applications. This review consolidates existing migrasomes, focusing role mechanisms use medical By examining structure function, this aims generate insights encourage further research, ultimately contributing advancements prevention treatment.

Язык: Английский

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Migrasomes: key players in immune regulation and promising medical applications

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Май 15, 2025

Migrasomes are newly discovered extracellular organelles released by migrating cells, such as immune tumor and other special functional cells like podocytes embryonic cells. They contain a diverse array of constituents, including proteins, lipids, RNA which can be to the designated location activate surrounding thereby facilitating intercellular communication signal transduction. Since then, our understanding mechanism function migrasomes has expanded exponentially, with recent evidence indicating they involved in various physiological pathological processes, particularly regulation. Furthermore, methods techniques for extracting, detecting, characterizing constantly advancing. Herein, we summarize current their key roles modulating responses, well prospective challenges clinical application, aiming provide novel insights into emerging organelles.

Язык: Английский

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Migrasome: a novel insight into unraveling physiological and pathological function

Molecular Biology Reports, Год журнала: 2025, Номер 52(1)

Опубликована: Май 28, 2025

Язык: Английский

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Research progress of migrasomes: from genesis to formation, physiology to pathology

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Авг. 14, 2024

Migrasomes are recently identified organelles that form at the ends or forks of retraction fibers (RFs) behind migrating cells and expelled from cell through migration. contain signaling molecules which captured by surrounding along with migrasomes released into extracellular environment following rupture migrasomes. Finally, action these molecules, facilitate entire process information conveyance. In addition, also serves as a “scavenger” removing damaged mitochondria to ensure cellular viability. Thus, play pivotal role in integration temporal, spatial, specific chemical clearance harmful substances, critical for grasping migrasomes’ functions. This review delves latest advancements research, covering aspects such discovery, distribution, structure characteristics, genesis regulation mechanisms, their correlation diseases. Additionally, we scrutinize present investigational findings on within cancer domain, examining potential impact prospective research avenues.

Язык: Английский

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