A new subtype of artificial cell‐derived vesicles from dental pulp stem cells with the bioequivalence and higher acquisition efficiency compared to extracellular vesicles

Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(7)

Опубликована: Июль 1, 2024

Abstract Extracellular vesicles (EVs) derived from dental pulp stem cells (DPSC) have been shown an excellent efficacy in a variety of disease models. However, current production methods fail to meet the needs clinical treatment. In this study, we present innovative approach substantially enhance ‘Artificial Cell‐Derived Vesicles (ACDVs)’ by extracting and purifying contents released DPSC lysate, namely intracellular vesicles. Comparative analysis was performed between ACDVs those obtained through ultracentrifugation. The extracted cell lysate general standard EVs similar protein secretion profile. new also significantly promoted wound healing, increased or decreased collagen regeneration, reduced inflammatory factors as EVs. More importantly, extraction efficiency is improved 16 times compared with using ultracentrifuge method. With its impressive attributes, subtype emerge prospective candidate for future applications regenerative medicine.

Язык: Английский

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Emerging roles of extracellular vesicles in oral and maxillofacial areas

International Journal of Oral Science, Год журнала: 2025, Номер 17(1)

Опубликована: Фев. 3, 2025

Abstract The oral and maxillofacial region is a highly complex area composed of multiple tissue types bears various critical functions the human body. Diseases in this pose significant diagnostic management challenges; therefore, exploring new strategies for early diagnosis, targeted treatment, reconstruction key to improving patient prognosis quality life. Extracellular vesicles are group heterogeneous lipid-bilayer membrane structures secreted by most cell types, including exosomes, microvesicles, apoptotic bodies. Present body fluids tissues, they act as messengers via transfer nucleic acids, proteins, metabolites recipient cells. To date, studies have revealed different roles extracellular physiological or pathological processes, well applications disease prognosis, treatment. importance specificity dental tissues indicate that derived from promising further research. This paper reviews published data on cells, fluids, regions, summarizes latest advances extensive sources, concludes with focus current research progress application prospects engineered exosomes science.

Язык: Английский

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Mesenchymal Stem Cells‐Derived Extracellular Vesicles Mimetics as Osteoinductive Mediators for Bone Healing

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 21, 2025

Abstract Bone defects remain challenging to treat, with common therapies still relying on invasive approaches. Mesenchymal stem cell‐derived extracellular vesicles (MSC‐EVs) offer a promising alternative due their regenerative and immunomodulatory properties, but challenges in scalable EV production limit clinical translation. Nanoghosts (NGs) are an emerging class of EV‐mimetics synthesized through the physical distortion ghost cells that innate bioactivity similar EVs while having more yields. In this study, osteogenic potential NGs made from MSC ghosts (MSC‐NGs) is explored for first time contrasted conventional MSC‐EVs. MSC‐NGs generated sonication, yielding two‐fold compared MSC‐EVs same number cells. Unlike MSC‐EVs, significantly enhanced differentiation MSCs, evidenced by increased alkaline phosphatase (ALP) activity early mineralization. Proteomic analysis further revealed enriched osteogenesis‐related proteins than vivo, treatment 0.5 mm mouse femoral osteotomy accelerated fracture healing, showing callus mineralization day 14 improved bone marrow reconstitution 21, along reduced osteoclastic activity. These findings demonstrate as effective therapeutics tissue engineering, offering advantages over future healing strategies.

Язык: Английский

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Dental pulp stem cell-derived intracellular vesicles prevent orthodontic relapse by inhibiting PI3K/Akt/NF-κB-mediated osteoclast activity

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 23, 2025

Orthodontic relapse, the undesired deviation of teeth from their corrected positions, remains a significant challenge in clinical orthodontics. Incomplete periodontal bone remodeling has been identified as key factor this process. Despite decades research, currently there are no effective strategies to prevent relapse. We isolated and dental pulp stem cell-derived intracellular vesicles (DPSC-IV) human tissue. To investigate its effect, DPSC-IV was added osteoblast or osteoclast differentiation medium. During orthodontic retention period, administrated rats by subgingival injection. Relapse distance relapse rate were calculated evaluate DPSC-IV's ability Additionally, Western blot analysis used examine inhibitory effect on differentiation. significantly promoted inhibited Application during resulted reduction both rate, with improved structure decreased activity. This mediated PI3K/Akt/NF-κB signaling pathway could be reversed PI3K activator insulin-like growth factor-1 (IGF-1). study highlights potential novel preventive approach against offering strategy for maintaining long-term stability.

Язык: Английский

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Tremella polysaccharide microneedles loaded with magnetic dental pulp stem cell intracellular vesicles used for androgenic alopecia

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Март 31, 2025

Mesenchymal stem cell exosomes are the most extensively researched type of Extracellular vesicles (EVs) that offer novel avenues for hair regeneration. However, their use in field regeneration was limited by poor production exosomes. It has been discovered intracellular (IVs), which produced at a higher rate than exosomes, play comparable biological purpose. As result, we developed HTMI-MN, microneedle uses tremella, Tremella polysaccharide (TPS), and hyaluronic acid (HA) as matrix materials. magnetic (Mag-IVs), work concert to treat androgenic alopecia (AGA) encourage growth. After characterization Mag-IVs, evaluated effect on angiogenesis scratch assay, Western Blot ELISA assay. In addition, tested protective Human papillary cells (HHDPCs) CCK-8 method, flow cytometry. Finally, effects tremella M1/M2 polarization macrophages were detected fluorescence staining, AGA model established vivo DHT, treatment given injection. Our study found Mag-IVs have greater power promote protect HHDPCs from apoptosis compared other vesicles. Besides, can make transformation anti-inflammatory phenotype. Taken together, experiments showed faster HTMI-MN-treated mice. These results indicate synergistically improve microenvironment, promising future treatment.

Язык: Английский

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Application of dental pulp stem cell-conditioned medium combined with deep crypreservation of autologous cranial flaps

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

Background Autologous cranial flaps preservation after decompressive craniectomy (DC) is crucial for cranioplasty, yet standard cryopreservation carries high complication rates (15-35%), primarily infections and bone resorption. These complications frequently necessitate surgical revisions increase morbidity risks. Current methods lack standardized solutions that simultaneously ensure osteocyte survival prevent microbial growth. Developing integrated bacteriostatic osteoprotective storage media remains an urgent unmet need to enhance patient outcomes. Objectives This study investigates optimized protocols autologous mitigate post-cranioplasty complications, while evaluating the preservative efficacy clinical translation potential of dental pulp stem cell-conditioned medium (DPSC-CM) as a novel osteogenic solution. Methods Dental cells (DPSCs) were cultured in serum-free generate DPSC-CM. To evaluate DPSC-CM, first, DPSC-CM was preliminarily evaluated by examining cell viability freezing resuscitation. Second, murine critical-size calvarial defect model surgically established. underwent 4-week experimental (DPSC-CM versus conventional cryoprotectants) reimplanted. Postoperative regeneration systematically quantified through high-resolution micro-CT analysis histomorphometric evaluation capacity. Given DPSC-CM's osteopreservative potential, vitro analyses confirmed osteogenic/angiogenic capacity proliferation/migration assays, differentiation, biomarker quantification. Results demonstrated superior preservation. Studies mouse defects showed preserved with combination deep (-196°C) significantly better healing cranioplasty than other groups, their neoangiogenic anti-inflammatory abilities also those groups. found be DPSCs osteogenesis embryonic osteoblast (MC3T3-E1 cells) angiogenesis human umbilical vein endothelial (HUVECs). Conclusions Considering superiority vascularization vivo vitro, well modulating local inflammatory microenvironment, synergistic emerges strategy preserving DC. multidisciplinary approach establishes transformative framework advancing technologies, demonstrating translational promise biological optimization traditional protocols.

Язык: Английский

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Intracellular vesicles: Novel nanovesicles superior to extracellular vesicles in translational medicine and clinical applications

Nano TransMed, Год журнала: 2024, Номер 3, С. 100044 - 100044

Опубликована: Авг. 15, 2024

Язык: Английский

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Artificial Nanovesicles Derived from Cells: A Promising Alternative to Extracellular Vesicles

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер unknown

Опубликована: Дек. 18, 2024

As naturally secreted vesicles by cells, extracellular (EVs) play essential roles in modulating cell–cell communication and have significant potential tissue regeneration, immune regulation, drug delivery. However, the low yield uncontrollable heterogeneity of EVs been obstacles to their widespread translation into clinical practice. Recently, it has discovered that artificial nanovesicles (NVs) produced cell processing can inherit components functions parent cells possess similar structures EVs, with significantly higher yields more flexible functionalization, making them a powerful complement natural EVs. This review focuses on recent advances research NVs as replacements for We provide an overview comparing summarize top-down preparation strategies NVs. The applications prepared from stem differentiated engineered are presented, well latest NV engineering. Finally, main challenges discussed.

Язык: Английский

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Revolutionizing Pressure Ulcer Regeneration: Unleashing the Potential of Extracellular Matrix-Derived Temperature-Sensitive Injectable Antioxidant Hydrogel for Superior Stem Cell Therapy

Biomaterials, Год журнала: 2024, Номер 314, С. 122880 - 122880

Опубликована: Окт. 5, 2024

Язык: Английский

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Dental pulp stem cells derived exosomes inhibit ferroptosis via regulating the Nrf2-keap1/GPX4 signaling pathway to ameliorate chronic kidney disease injury

Tissue and Cell, Год журнала: 2024, Номер 93, С. 102670 - 102670

Опубликована: Дек. 7, 2024

Язык: Английский

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