Analytical Chemistry, Год журнала: 2024, Номер 97(1), С. 741 - 748

Опубликована: Дек. 30, 2024

High-performance isolation of exosomes as a promising liquid biopsy target is great importance for both fundamental research and clinical applications. This is, however, challenged by the prevalent heterogeneity highly complex nature biosamples. Here, we introduce use CD81-targeting peptide building block tailoring molecular baits exosome payload analysis in biofluids. To explore full potential multivalent interactions, peptide-functionalized affinity interfaces were covalently engineered with varied assembling topology, flexibility, local density recognition motif. Capable best fitting surface conformation CD81 on curved membranes, dual-layered capture interface (Exo-PepTrap2) tandem bivalent decoration outperforms monolayered branched architectures. Enabled multivalency-enhanced reaction antifouling ability, Exo-PepTrap2 achieved high yield purity targeted fishing cell culture media urine samples. By integration mass spectrometry-based proteomic profiling, differentially expressed proteins efficiently identified harvested biomarkers gastrointestinal adenocarcinoma. CD81-targeted platform provides new viewpoint multivalency-based versatile tool to information precise medicine.

Язык: Английский