Engineering Peptide-Based Molecular Baits for Targeted Fishing and Protein Profiling of Exosomes as a Liquid Biopsy for Gastrointestinal Adenocarcinoma DOI
Kun Xu, Yanting Hao, Han Gao

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 97(1), P. 741 - 748

Published: Dec. 30, 2024

High-performance isolation of exosomes as a promising liquid biopsy target is great importance for both fundamental research and clinical applications. This is, however, challenged by the prevalent heterogeneity highly complex nature biosamples. Here, we introduce use CD81-targeting peptide building block tailoring molecular baits exosome payload analysis in biofluids. To explore full potential multivalent interactions, peptide-functionalized affinity interfaces were covalently engineered with varied assembling topology, flexibility, local density recognition motif. Capable best fitting surface conformation CD81 on curved membranes, dual-layered capture interface (Exo-PepTrap2) tandem bivalent decoration outperforms monolayered branched architectures. Enabled multivalency-enhanced reaction antifouling ability, Exo-PepTrap2 achieved high yield purity targeted fishing cell culture media urine samples. By integration mass spectrometry-based proteomic profiling, differentially expressed proteins efficiently identified harvested biomarkers gastrointestinal adenocarcinoma. CD81-targeted platform provides new viewpoint multivalency-based versatile tool to information precise medicine.

Language: Английский

Innovating intervertebral disc degeneration therapy: Harnessing the power of extracellular vesicles DOI Creative Commons
Shanfeng Chen,

Yiming Dou,

Yiming Zhang

et al.

Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: 50, P. 44 - 55

Published: Jan. 1, 2025

Language: Английский

Citations

1
Extracellular vesicles as natural nanocarriers: From in vitro engineering to in situ generation in cancer therapy DOI
Teng Zhang,

Zimai Liu,

Yuanyuan Wei

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 161653 - 161653

Published: March 1, 2025

Language: Английский

Citations

0
Quantifying the Shape and Stiffness of Single Extracellular Vesicles in Aqueous Solution via Membrane Diffusivity Measurements DOI Creative Commons
Yihan Wang,

Huihui Gao,

Chu Han

et al.

Chemical & Biomedical Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Language: Английский

Citations

0
Engineering Peptide-Based Molecular Baits for Targeted Fishing and Protein Profiling of Exosomes as a Liquid Biopsy for Gastrointestinal Adenocarcinoma DOI
Kun Xu, Yanting Hao, Han Gao

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 97(1), P. 741 - 748

Published: Dec. 30, 2024

High-performance isolation of exosomes as a promising liquid biopsy target is great importance for both fundamental research and clinical applications. This is, however, challenged by the prevalent heterogeneity highly complex nature biosamples. Here, we introduce use CD81-targeting peptide building block tailoring molecular baits exosome payload analysis in biofluids. To explore full potential multivalent interactions, peptide-functionalized affinity interfaces were covalently engineered with varied assembling topology, flexibility, local density recognition motif. Capable best fitting surface conformation CD81 on curved membranes, dual-layered capture interface (Exo-PepTrap2) tandem bivalent decoration outperforms monolayered branched architectures. Enabled multivalency-enhanced reaction antifouling ability, Exo-PepTrap2 achieved high yield purity targeted fishing cell culture media urine samples. By integration mass spectrometry-based proteomic profiling, differentially expressed proteins efficiently identified harvested biomarkers gastrointestinal adenocarcinoma. CD81-targeted platform provides new viewpoint multivalency-based versatile tool to information precise medicine.

Language: Английский

Citations

1