Frontiers in Cardiovascular Medicine,
Год журнала:
2024,
Номер
11
Опубликована: Дек. 19, 2024
Extracellular
vesicles
(EVs)
are
nanosized
particles
secreted
by
cells
that
play
crucial
roles
in
intercellular
communication,
especially
the
context
of
cardiovascular
diseases
(CVDs).
These
carry
complex
cargo,
including
proteins,
lipids,
and
nucleic
acids,
reflects
physiological
or
pathological
state
their
origin.
Multiomics
analysis
cell-derived
EVs
has
provided
valuable
insights
into
molecular
mechanisms
underlying
CVDs
identifying
specific
proteins
EV-bound
targets
involved
disease
progression.
Recent
studies
have
demonstrated
engineered
EVs,
which
designed
to
therapeutic
molecules
modified
enhance
targeting
capabilities,
hold
promise
for
treating
CVDs.
Analysis
EV
proteome
been
instrumental
key
can
be
targeted
modulated
within
these
vesicles.
For
example,
inflammation,
thrombosis,
cardiac
remodeling
identified
as
potential
targets.
Furthermore,
engineering
increase
delivery
tissues,
such
myocardium,
modulate
immunogenicity
efficacy
is
an
emerging
area
research.
By
leveraging
gained
from
multiomics
analyses,
researchers
developing
EV-based
therapies
selectively
target
processes
CVDs,
offering
a
novel
potentially
more
effective
treatment
strategy.
This
review
integrates
core
findings
highlights
applications.
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Март 8, 2025
The
liver,
functioning
as
an
endocrine
organ,
secretes
a
variety
of
substances
that
influence
the
activities
other
body
organs.
Conversely,
molecules
generated
by
organs
such
bone,
gut,
and
adipose
tissue
can
also
impact
liver
function.
Accumulating
evidence
suggests
bidirectional
communication
between
However,
research
on
how
extracellular
vesicles
(EVs),
which
transport
active
molecular
mediators,
contribute
to
this
interorgan
is
still
in
its
nascent
stages.
EVs
are
capable
transporting
functional
molecules,
including
lipids,
nucleic
acids,
proteins,
thereby
affecting
recipient
cells
across
different
at
biological
level.
This
review
examines
role
facilitating
cardiovascular
system,
pancreas,
brain,
lungs,
kidneys,
tissue.
It
explores
their
potential
disease
treatment
highlights
challenges
understanding
EV-mediated
interactions.
contribution
mediator-carrying
two-way
remains
area
ongoing
investigation.
Future
will
provide
more
comprehensive
theoretical
foundation
clarify
precise
mechanisms
governing
organs,
pinpoint
medical
targets,
expand
application
within
realm
precision
medicine.
Genes,
Год журнала:
2025,
Номер
16(3), С. 330 - 330
Опубликована: Март 12, 2025
Extracellular
vesicles
(EVs)
are
nanovesicles
that
facilitate
intercellular
communication
by
carrying
essential
biomolecules
under
physiological
and
pathological
conditions
including
microRNAs
(miRNAs).
They
found
in
various
body
fluids,
such
as
blood,
urine,
saliva,
their
levels
fluctuate
with
disease
progression,
making
them
valuable
diagnostic
tools.
However,
isolating
EVs
is
challenging
due
to
small
size
biological
complexity.
Here,
we
summarize
the
principles
behind
most
common
EV
isolation
methods
ultracentrifugation,
precipitation,
immunoaffinity,
sorting,
ultrafiltration,
exclusion
chromatography,
microfluidics
while
highlighting
protocol
strengths
weaknesses.
We
also
review
main
strategies
identify
quantify
circulating
miRNAs
a
particular
focus
on
EV-encapsulated
miRNAs.
Since
these
hold
special
clinical
interest
derived
from
superior
stability
therapeutic
potential,
information
provided
here
should
provide
guidance
for
future
research
initiatives
promising
field
of
treatment
based
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Апрель 1, 2025
Extracellular
particles
(EPs),
including
extracellular
vesicles
(EVs)
and
non-vesicular
(NVEPs),
are
multimolecular
biomaterials
released
by
cells
that
play
a
crucial
role
in
intercellular
communication.
Recently,
new
subtypes
of
EPs
associated
with
central
nervous
system
(CNS),
such
as
exophers
supermeres
have
been
identified.
These
provide
perspectives
for
understanding
the
pathological
progression
CNS
disorders
confer
potential
diagnostic
value
liquid
biopsies
neurodegenerative
diseases
(NDs).
Moreover,
emerged
promising
drug
delivery
vehicles
targeted
platforms
CNS-specific
therapies.
In
this
review,
we
delineate
landscape
EP
their
roles
pathophysiology
diseases.
We
also
review
recent
advances
EP-based
diagnosis
NDs
highlight
importance
analytical
single-particle
resolution
exploitation
biomarkers.
Furthermore,
summarize
application
engineered
EVs
treatment
outline
underexplored
NVEPs
novel
therapeutic
agents.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 18, 2024
As
naturally
secreted
vesicles
by
cells,
extracellular
(EVs)
play
essential
roles
in
modulating
cell–cell
communication
and
have
significant
potential
tissue
regeneration,
immune
regulation,
drug
delivery.
However,
the
low
yield
uncontrollable
heterogeneity
of
EVs
been
obstacles
to
their
widespread
translation
into
clinical
practice.
Recently,
it
has
discovered
that
artificial
nanovesicles
(NVs)
produced
cell
processing
can
inherit
components
functions
parent
cells
possess
similar
structures
EVs,
with
significantly
higher
yields
more
flexible
functionalization,
making
them
a
powerful
complement
natural
EVs.
This
review
focuses
on
recent
advances
research
NVs
as
replacements
for
We
provide
an
overview
comparing
summarize
top-down
preparation
strategies
NVs.
The
applications
prepared
from
stem
differentiated
engineered
are
presented,
well
latest
NV
engineering.
Finally,
main
challenges
discussed.
Bioengineering & Translational Medicine,
Год журнала:
2025,
Номер
10(3)
Опубликована: Янв. 7, 2025
Mesenchymal
stem
cell-derived
extracellular
vesicles
(MSC
EVs)
are
an
attractive
therapeutic
option
for
regenerative
medicine
applications
due
to
their
inherently
pro-angiogenic
and
anti-inflammatory
properties.
However,
reproducible
cost-effective
production
of
highly
potent
MSC
EVs
is
challenging,
limiting
translational
potential.
Here,
we
investigated
whether
the
well-characterized
responsiveness
MSCs
mechanical
environment-specifically,
substrate
stiffness-could
be
exploited
generate
with
increased
bioactivity
without
need
biochemical
priming
or
genetic
manipulation.
Using
polydimethylsiloxane
bone
marrow-derived
(BM-MSCs),
show
that
decreasing
stiffness
substrates
as
low
3
kPa
significantly
improves
measured
by
tube
formation
gap
closure
assays.
We
also
demonstrate
lower
EV
overall
yield,
important
clinical
translation.
Furthermore,
establish
mechanoresponsiveness
induced
pluripotent
(iMSC)
comparability
BM-MSC
EVs,
again
using
With
this
data,
confirm
iMSCs'
feasibility
alternative,
renewable
cell
source
reduced
donor
variability.
Overall,
these
results
suggest
utilizing
a
promising,
simple,
potentially
scalable
approach
does
not
require
exogenous
cargo
extraneous
reagents
EVs.
Extracellular
vesicles
(EVs)
are
nanosized,
membrane-bound
structures
that
have
emerged
as
promising
tools
for
drug
delivery,
especially
in
the
treatment
of
lysosomal
storage
disorders
(LSDs)
with
central
nervous
system
(CNS)
involvement.
This
review
highlights
unique
properties
EVs,
such
their
biocompatibility,
capacity
to
cross
blood-brain
barrier
(BBB),
and
potential
therapeutic
cargo
loading,
including
enzymes
genetic
material.
Current
therapies
LSDs,
like
enzyme
replacement
therapy
(ERT),
often
fail
address
neurological
symptoms
due
inability
BBB.
EVs
offer
a
viable
alternative,
allowing
targeted
delivery
CNS
improving
outcomes.
We
discuss
recent
advancements
engineering
modification
enhance
targeting,
circulation
time
stability,
provide
detailed
overview
application
Gaucher
Fabry
diseases,
Sanfilippo
syndrome.
Despite
potential,
challenges
remain
scaling
production,
ensuring
isolation
purity,
meeting
regulatory
requirements.
Future
developments
will
focus
on
overcoming
these
barriers,
paving
way
clinical
translation
EV-based
LSDs
other
disorders.
Biomaterials Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
This
review
summarizes
naïve
extracellular
vesicles
(EVs)
in
clinical
trials
for
central
nervous
system
(CNS)
diseases
and
updates
recent
translational
preclinical
research
on
EV-loaded
drugs
or
genes
CNS
treatments.
