Engineering extracellular vesicles for targeted therapeutics in cardiovascular disease DOI Creative Commons
Enze Fu, Kai Pan, Zongjin Li

и другие.

Frontiers in Cardiovascular Medicine, Год журнала: 2024, Номер 11

Опубликована: Дек. 19, 2024

Extracellular vesicles (EVs) are nanosized particles secreted by cells that play crucial roles in intercellular communication, especially the context of cardiovascular diseases (CVDs). These carry complex cargo, including proteins, lipids, and nucleic acids, reflects physiological or pathological state their origin. Multiomics analysis cell-derived EVs has provided valuable insights into molecular mechanisms underlying CVDs identifying specific proteins EV-bound targets involved disease progression. Recent studies have demonstrated engineered EVs, which designed to therapeutic molecules modified enhance targeting capabilities, hold promise for treating CVDs. Analysis EV proteome been instrumental key can be targeted modulated within these vesicles. For example, inflammation, thrombosis, cardiac remodeling identified as potential targets. Furthermore, engineering increase delivery tissues, such myocardium, modulate immunogenicity efficacy is an emerging area research. By leveraging gained from multiomics analyses, researchers developing EV-based therapies selectively target processes CVDs, offering a novel potentially more effective treatment strategy. This review integrates core findings highlights applications.

Язык: Английский

Unravelling exosome paradigm: Therapeutic, diagnostic and theranostics application and regulatory consideration DOI
Reetika Tandon, Nidhi Srivastava

Life Sciences, Год журнала: 2025, Номер unknown, С. 123472 - 123472

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1

Extracellular vesicle-mediated bidirectional communication between the liver and other organs: mechanistic exploration and prospects for clinical applications DOI Creative Commons
Wenhui Mo, Yunke Peng,

Yanyi Zheng

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Март 8, 2025

The liver, functioning as an endocrine organ, secretes a variety of substances that influence the activities other body organs. Conversely, molecules generated by organs such bone, gut, and adipose tissue can also impact liver function. Accumulating evidence suggests bidirectional communication between However, research on how extracellular vesicles (EVs), which transport active molecular mediators, contribute to this interorgan is still in its nascent stages. EVs are capable transporting functional molecules, including lipids, nucleic acids, proteins, thereby affecting recipient cells across different at biological level. This review examines role facilitating cardiovascular system, pancreas, brain, lungs, kidneys, tissue. It explores their potential disease treatment highlights challenges understanding EV-mediated interactions. contribution mediator-carrying two-way remains area ongoing investigation. Future will provide more comprehensive theoretical foundation clarify precise mechanisms governing organs, pinpoint medical targets, expand application within realm precision medicine.

Язык: Английский

Процитировано

1

Methods for Extracellular Vesicle Isolation: Relevance for Encapsulated miRNAs in Disease Diagnosis and Treatment DOI Open Access
María Ljungström, Elisa Oltra

Genes, Год журнала: 2025, Номер 16(3), С. 330 - 330

Опубликована: Март 12, 2025

Extracellular vesicles (EVs) are nanovesicles that facilitate intercellular communication by carrying essential biomolecules under physiological and pathological conditions including microRNAs (miRNAs). They found in various body fluids, such as blood, urine, saliva, their levels fluctuate with disease progression, making them valuable diagnostic tools. However, isolating EVs is challenging due to small size biological complexity. Here, we summarize the principles behind most common EV isolation methods ultracentrifugation, precipitation, immunoaffinity, sorting, ultrafiltration, exclusion chromatography, microfluidics while highlighting protocol strengths weaknesses. We also review main strategies identify quantify circulating miRNAs a particular focus on EV-encapsulated miRNAs. Since these hold special clinical interest derived from superior stability therapeutic potential, information provided here should provide guidance for future research initiatives promising field of treatment based

Язык: Английский

Процитировано

1

Extracellular particles: emerging insights into central nervous system diseases DOI Creative Commons
Shenyuan Chen, Qiaoliang Bao,

Wenrong Xu

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 1, 2025

Extracellular particles (EPs), including extracellular vesicles (EVs) and non-vesicular (NVEPs), are multimolecular biomaterials released by cells that play a crucial role in intercellular communication. Recently, new subtypes of EPs associated with central nervous system (CNS), such as exophers supermeres have been identified. These provide perspectives for understanding the pathological progression CNS disorders confer potential diagnostic value liquid biopsies neurodegenerative diseases (NDs). Moreover, emerged promising drug delivery vehicles targeted platforms CNS-specific therapies. In this review, we delineate landscape EP their roles pathophysiology diseases. We also review recent advances EP-based diagnosis NDs highlight importance analytical single-particle resolution exploitation biomarkers. Furthermore, summarize application engineered EVs treatment outline underexplored NVEPs novel therapeutic agents.

Язык: Английский

Процитировано

1

Global Requirements for Manufacturing and Validation of Clinical Grade Extracellular Vesicles DOI Creative Commons
Abhimanyu Thakur, Deepika Rai

The Journal of Liquid Biopsy, Год журнала: 2024, Номер 6, С. 100278 - 100278

Опубликована: Ноя. 20, 2024

Язык: Английский

Процитировано

6

Artificial Nanovesicles Derived from Cells: A Promising Alternative to Extracellular Vesicles DOI

Saiyun Lou,

Wei Hu,

Pengyao Wei

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер unknown

Опубликована: Дек. 18, 2024

As naturally secreted vesicles by cells, extracellular (EVs) play essential roles in modulating cell–cell communication and have significant potential tissue regeneration, immune regulation, drug delivery. However, the low yield uncontrollable heterogeneity of EVs been obstacles to their widespread translation into clinical practice. Recently, it has discovered that artificial nanovesicles (NVs) produced cell processing can inherit components functions parent cells possess similar structures EVs, with significantly higher yields more flexible functionalization, making them a powerful complement natural EVs. This review focuses on recent advances research NVs as replacements for We provide an overview comparing summarize top-down preparation strategies NVs. The applications prepared from stem differentiated engineered are presented, well latest NV engineering. Finally, main challenges discussed.

Язык: Английский

Процитировано

5

Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness DOI Creative Commons

Emily H. Powsner,

Stephanie M. Kronstadt,

Kristin Nikolov

и другие.

Bioengineering & Translational Medicine, Год журнала: 2025, Номер 10(3)

Опубликована: Янв. 7, 2025

Mesenchymal stem cell-derived extracellular vesicles (MSC EVs) are an attractive therapeutic option for regenerative medicine applications due to their inherently pro-angiogenic and anti-inflammatory properties. However, reproducible cost-effective production of highly potent MSC EVs is challenging, limiting translational potential. Here, we investigated whether the well-characterized responsiveness MSCs mechanical environment-specifically, substrate stiffness-could be exploited generate with increased bioactivity without need biochemical priming or genetic manipulation. Using polydimethylsiloxane bone marrow-derived (BM-MSCs), show that decreasing stiffness substrates as low 3 kPa significantly improves measured by tube formation gap closure assays. We also demonstrate lower EV overall yield, important clinical translation. Furthermore, establish mechanoresponsiveness induced pluripotent (iMSC) comparability BM-MSC EVs, again using With this data, confirm iMSCs' feasibility alternative, renewable cell source reduced donor variability. Overall, these results suggest utilizing a promising, simple, potentially scalable approach does not require exogenous cargo extraneous reagents EVs.

Язык: Английский

Процитировано

0

Extracellular Vesicles as Tools for Crossing the Blood–Brain Barrier to Treat Lysosomal Storage Diseases DOI Creative Commons

Giovanni Lerussi,

Verónica Villagrasa-Araya,

Marc Moltó‐Abad

и другие.

Life, Год журнала: 2025, Номер 15(1), С. 70 - 70

Опубликована: Янв. 9, 2025

Extracellular vesicles (EVs) are nanosized, membrane-bound structures that have emerged as promising tools for drug delivery, especially in the treatment of lysosomal storage disorders (LSDs) with central nervous system (CNS) involvement. This review highlights unique properties EVs, such their biocompatibility, capacity to cross blood-brain barrier (BBB), and potential therapeutic cargo loading, including enzymes genetic material. Current therapies LSDs, like enzyme replacement therapy (ERT), often fail address neurological symptoms due inability BBB. EVs offer a viable alternative, allowing targeted delivery CNS improving outcomes. We discuss recent advancements engineering modification enhance targeting, circulation time stability, provide detailed overview application Gaucher Fabry diseases, Sanfilippo syndrome. Despite potential, challenges remain scaling production, ensuring isolation purity, meeting regulatory requirements. Future developments will focus on overcoming these barriers, paving way clinical translation EV-based LSDs other disorders.

Язык: Английский

Процитировано

0

Extracellular vesicles as drug and gene delivery vehicles in central nervous system diseases DOI Creative Commons

Xi Shi,

Weilong He,

Ashwin Gupta

и другие.

Biomaterials Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

This review summarizes naïve extracellular vesicles (EVs) in clinical trials for central nervous system (CNS) diseases and updates recent translational preclinical research on EV-loaded drugs or genes CNS treatments.

Язык: Английский

Процитировано

0

Extracellular vesicles in uveal melanoma - biological roles and diagnostic value DOI Creative Commons
Ornella Urzì, Roger Olofsson Bagge, Rossella Crescitelli

и другие.

Cancer Letters, Год журнала: 2025, Номер unknown, С. 217531 - 217531

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0