Descriptive Comparative Transcriptomic Analysis of Genotype IV SHEV ORF3-Expressing HepG2 Cells

Microorganisms, Год журнала: 2025, Номер 13(2), С. 412 - 412

Опубликована: Фев. 13, 2025

Background: Swine hepatitis E (HEV) is a zoonotic infectious disease caused by the swine virus (SHEV). Open reading frame 3 (ORF3) key virulence factor in HEV, playing crucial role release of viral particles, modulation host innate immune response, and regulation autophagy apoptosis, etc. However, its main function pathogenic mechanism remain incompletely understood. Results: In our study, adenoviruses ADV4-ORF3 ADV4-GFP were successfully constructed mediated overexpression enhanced green fluorescent protein (EGFP)-ORF3 EGFP HepG2 cells. A total 217 differentially expressed messenger RNAs (mRNAs) screened high-throughput sequencing, 27 statistically significant genes for further quantitative real-time reverse transcription (qRT-PCR) verification functional enrichment (Gene Ontology [GO] Kyoto Encyclopedia Genes Genomes [KEGG]). They are mainly involved six pathways: cellular response to unfolded protein, inflammatory cytokine activity, TNF signaling pathway, influenza A, pathways cancer. comparative analysis transcriptome mRNA expression profiles lncRNA results showed that mRNAs GPX1, MDM4, CLDN 39 transcripts overlapped have been identified. Conclusions: Eight differential genes, HSPA1A, HSPA1B, PLD3, RELA, GPI, SAMHD1, RPS6KA4, PIK3CB, verified. Comparing analyzing two sequencing methods indicated identified SHEV ORF3-expressing cells, which has laid genetic foundation physiological ORF3.

Язык: Английский

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Multiple Functions of Hepatitis E Virus ORF3

Microorganisms, Год журнала: 2024, Номер 12(7), С. 1405 - 1405

Опубликована: Июль 11, 2024

Hepatitis E (Hepatitis E, HE) is an acute and chronic infectious hepatitis caused by virus Virus, HEV) infection, which responsible for most in the world a significant public health problem. The pathogen, HEV, has three Open Reading Frames (ORFs) ORF1, ORF2, ORF3, each of different function. Most current research focused on ORF1 while ORF3 still relatively small. To provide more ideas study HEV pathogenesis prevention treatment HE, this paper reviews effects ERK pathway, growth factors, immune response, release.

Язык: Английский

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Hepatitis E and Potential Public Health Implications from a One-Health Perspective: Special Focus on the European Wild Boar (Sus scrofa)

Pathogens, Год журнала: 2024, Номер 13(10), С. 840 - 840

Опубликована: Сен. 27, 2024

The hepatitis E virus (HEV) has become increasingly important in recent years terms of risk for public health, as the main causative agent acute viral hepatitis. It is a foodborne disease transmitted to humans through consumption contaminated water or food. Human-to-human transmission sporadic and linked transfusions transplants. reservoirs are domestic pigs wild boars, although, compared pigs, boars represent lesser source since their population smaller derived products more limited. These peculiarities often make role boar reservoir spread underestimated. As health problem that involves several animal species humans, management requires an interdisciplinary approach, concept “One Health” must be addressed. In this direction, present review intends analyze E, with particular focus on boar. For purpose, literature data have been collected from different scientific search engines: PubMed, MEDLINE, Google scholar, keywords such “HEV epidemiology”, “Extrahepatic manifestations Hepatitis E”, infection control measures”, among others, used. first part, manuscript provides general information disease, epidemiology, methods, clinical implications health. second it addresses detail related epidemiology. document will useful all those who intend infectious “One-Health” perspective.

Язык: Английский

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Comprehensive analysis of mRNA Transcriptome Profiles in HepG2 Cells Expressing Genotype IV Swine Hepatitis E Virus ORF3

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 9, 2024

Background Porcine hepatitis E is a zoonotic infectious disease caused by swine virus (HEV), open reading frames 3 an important virulence protein of porcine HEV, which plays role in the release viral particles and host innate immune response, regulation autophagy apoptosis, etc., but its main function pathogenic mechanism are not perfect current research. Results In our study, adenoviruses ADV4-ORF3 ADV4-GFP were successfully constructed mediated overexpression enhanced green fluorescent (EGFP)-ORF3 EGFP HepG2 cells. A total 217 differentially expressed messenger RNAs (mRNAs) screened high-throughput sequencing, 27 statistically significant genes for further quantitative real-time reverse transcription (qRT-PCR) verification functional enrichment (Gene Ontology [GO] Kyoto Encyclopedia Genes Genomes [KEGG]). They mainly involved 6 pathways: cellular response to unfolded protein, Inflammatory cytokine activity, TNF signaling pathway, Influenza A, Pathways cancer. Conclusions The differential verified, laid genetic foundation physiological HEV ORF3

Язык: Английский

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Deciphering the hepatitis E virus ORF1: Functional Domains, Protein Processing, and Patient-Derived Mutations

Virology, Год журнала: 2024, Номер 603, С. 110350 - 110350

Опубликована: Дек. 7, 2024

Язык: Английский

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