The temporal pattern of intracortical microstimulation pulses elicits distinct temporal and spatial recruitment of cortical neuropil and neurons DOI

James R. Eles,

Kevin C. Stieger, Takashi D.Y. Kozai

и другие.

Journal of Neural Engineering, Год журнала: 2020, Номер 18(1), С. 015001 - 015001

Опубликована: Окт. 19, 2020

Abstract Objective. The temporal spacing or distribution of stimulation pulses in therapeutic neurostimulation waveforms—referred to here as the Temporal Pattern (TP)—has emerged an important parameter for tuning response deep-brain and intracortical microstimulation (ICMS). While it has long been assumed that modulating TP ICMS may be effective by altering rate coding neural response, is unclear how alters at network level. present study designed elucidate Approach . We use vivo two-photon imaging mice expressing calcium sensor Thy1 -GCaMP glutamate hSyn -iGluSnFr examine layer II/III with different TPs. neuronal TPs same average frequency (10 Hz) total charge injection, but varying degrees bursting. also investigate one control pattern 100 Hz 10X injection. Main Results Stimulation trains injection distinct recruit sets neurons. More than half (60% 309 cells) neurons prefer over other. Despite their spatial recruitment patterns, cells exhibit similar ability follow 30 s both without failing, they levels release during stimulation. Both entrain bursting pattern, a ∼21-fold increase relative power Bursting results statistically significant elevation correlation between somatic activity neuropil activity, which we explore metric inhibitory-excitatory tone. Interestingly, soma-neuropil predictor cell preference TP, exposes key link Finally, using mesoscale imaging, show result distal inhibition stimulation, reveals complex interactions tone ICMS. Significance Our ultimately suggest valuable space modulate This presents broader mechanism action coding, previously thought. By implicating these additional mechanisms, have utility clinic should pursued expand efficacy therapies.

Язык: Английский

Characterizing the short-latency evoked response to intracortical microstimulation across a multi-electrode array DOI Creative Commons
Joseph Sombeck, Juliet Heye, Karthik Kumaravelu

и другие.

Journal of Neural Engineering, Год журнала: 2022, Номер 19(2), С. 026044 - 026044

Опубликована: Апрель 1, 2022

Persons with tetraplegia can use brain-machine interfaces to make visually guided reaches robotic arms. Without somatosensory feedback, these movements will likely be slow and imprecise, like those of persons who retain movement but have lost proprioception. Intracortical microstimulation (ICMS) has promise for providing artificial feedback. ICMS that mimics naturally occurring neural activity, may allow afferent are more informative easier learn than stimulation evoking unnaturalistic activity. To develop such biomimetic patterns, it is important characterize the responses neurons ICMS.

Язык: Английский

Процитировано

34
Self‐Powered Programming of Fibroblasts into Neurons via a Scalable Magnetoelastic Generator Array DOI
Alberto Libanori, Jennifer Soto, Jing Xu

и другие.

Advanced Materials, Год журнала: 2022, Номер 35(7)

Опубликована: Дек. 5, 2022

Developing scalable electrical stimulating platforms for cell and tissue engineering applications is limited by external power source dependency, wetting resistance, microscale size requirements, suitable flexibility. Here, a versatile platform developed to enable tunable stimulation biological harnessing the giant magnetoelastic effect in soft systems, converting gentle air pressure (100-400 kPa) yield current of up 10.5 mA voltage 9.5 mV. The can be easily manufactured scaled integration multiwell plates via 3D printing. authors demonstrate that generated this enhances conversion fibroblasts into neurons 2-fold (104%) subsequent neuronal maturation 3-fold (251%). This configurable device has broad high throughput organ-on-a-chip paves way future development neural engineering, including cellular therapy implantable self-powered devices.

Язык: Английский

Процитировано

34
In vivo spatiotemporal dynamics of astrocyte reactivity following neural electrode implantation DOI
Sajishnu Savya, Fan Li, Stephanie Lam

и другие.

Biomaterials, Год журнала: 2022, Номер 289, С. 121784 - 121784

Опубликована: Сен. 2, 2022

Язык: Английский

Процитировано

31
Activity-dependent recruitment of inhibition and excitation in the awake mammalian cortex during electrical stimulation DOI Creative Commons
Maria C. Dadarlat, Yujiao Jennifer Sun, Michael P. Stryker

и другие.

Neuron, Год журнала: 2023, Номер 112(5), С. 821 - 834.e4

Опубликована: Дек. 21, 2023

Язык: Английский

Процитировано

19
Dynamic amplitude modulation of microstimulation evokes biomimetic onset and offset transients and reduces depression of evoked calcium responses in sensory cortices DOI Creative Commons
Christopher Hughes, Takashi D.Y. Kozai

Brain stimulation, Год журнала: 2023, Номер 16(3), С. 939 - 965

Опубликована: Май 1, 2023

Intracortical microstimulation (ICMS) is an emerging approach to restore sensation people with neurological injury or disease. Biomimetic microstimulation, stimulus trains that mimic neural activity in the brain through encoding of onset and offset transients, could improve utility ICMS for brain-computer interface (BCI) applications, but how biomimetic affects activation not understood. Current "biomimetic" aim reproduce strong transients evoked by sensory input dynamic modulation parameters. Stimulus induced depression (decreases intensity over time) also a potential barrier clinical implementation feedback, may reduce this effect.

Язык: Английский

Процитировано

18
The frequency of cortical microstimulation shapes artificial touch DOI Creative Commons
Thierri Callier, Nathan W. Brantly,

Attilio Caravelli

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2019, Номер 117(2), С. 1191 - 1200

Опубликована: Дек. 26, 2019

Intracortical microstimulation (ICMS) of the somatosensory cortex evokes vivid tactile sensations and can be used to convey sensory feedback from brain-controlled bionic hands. Changes in ICMS frequency lead changes resulting sensation, but discriminability has only been investigated over a narrow range low frequencies. Furthermore, correlates remain poorly understood. Specifically, it remains elucidated whether modulate sensation magnitude-as do amplitude-or they also quality sensation. To fill these gaps, we trained monkeys discriminate pulse trains wide frequencies (from 10 400 Hz). amplitude varied across stimuli dissociate magnitude ensure that animals could not make judgments based on magnitude. We found consistently up ∼200 Hz were highly electrode dependent: On some electrodes, perceptually distinguishable amplitude-seemingly giving rise change quality; others, not. discuss implications our findings for neural coding

Язык: Английский

Процитировано

53
Intracellular ATP levels in mouse cortical excitatory neurons varies with sleep–wake states DOI Creative Commons
Akiyo Natsubori, Tomomi Tsunematsu, Akihiro Karashima

и другие.

Communications Biology, Год журнала: 2020, Номер 3(1)

Опубликована: Сен. 7, 2020

Whilst the brain is assumed to exert homeostatic functions keep cellular energy status constant under physiological conditions, this has not been experimentally proven. Here, we conducted in vivo optical recordings of intracellular concentration adenosine 5'-triphosphate (ATP), major metabolite, using a genetically encoded sensor mouse brain. We demonstrate that ATP levels cortical excitatory neurons fluctuate cortex-wide manner depending on sleep-wake states, correlating with arousal. Interestingly, profoundly decreased during rapid eye movement sleep, suggesting negative balance despite simultaneous increase cerebral hemodynamics for supply. The reduction was also observed response local electrical stimulation neuronal activation, whereas were simultaneously enhanced. These observations indicate metabolism may always meet demands, consequently resulting fluctuations neurons.

Язык: Английский

Процитировано

41
Invasive and Non-invasive Neurostimulation for OCD DOI
Isidoor O. Bergfeld, Eva Dijkstra, Ilse Graat

и другие.

Current topics in behavioral neurosciences, Год журнала: 2021, Номер unknown, С. 399 - 436

Опубликована: Янв. 1, 2021

Язык: Английский

Процитировано

40
MesoNet allows automated scaling and segmentation of mouse mesoscale cortical maps using machine learning DOI Creative Commons
Dongsheng Xiao, Brandon J. Forys, Matthieu P. Vanni

и другие.

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Окт. 13, 2021

Abstract Understanding the basis of brain function requires knowledge cortical operations over wide spatial scales and quantitative analysis activity in well-defined regions. Matching an anatomical atlas to functional data substantial labor expertise. Here, we developed automated machine learning-based registration segmentation approach for mouse mesoscale images. A deep learning model identifies nine landmarks using only a single raw fluorescent image. Another fully convolutional network was adapted delimit boundaries. This alignment extended by adding three approaches that use sensory maps or spatial-temporal motifs. We present this methodology as MesoNet, robust user-friendly pipeline pre-trained models segment regions defined Allen Mouse Brain Atlas. Python-based toolbox can also be combined with existing methods facilitate high-throughput analysis.

Язык: Английский

Процитировано

39
Effects of stimulus pulse rate on somatosensory adaptation in the human cortex DOI Creative Commons
Christopher Hughes, Sharlene N. Flesher, Robert A. Gaunt

и другие.

Brain stimulation, Год журнала: 2022, Номер 15(4), С. 987 - 995

Опубликована: Июнь 4, 2022

Intracortical microstimulation (ICMS) of the somatosensory cortex can restore sensation to people with neurological diseases. However, many aspects ICMS are poorly understood, including effect stimulation on percept intensity over time.Here, we evaluate how tactile percepts evoked by in a human participant adapt time.We delivered continuous and intermittent assessed reported time participant. Experiments were conducted approximately one year linear mixed effects models used assess significance.Continuous at high frequencies led rapid decreases intensity, while low frequency maintained for longer periods. Burst-modulated extended before began decrease, but all protocols ultimately resulted complete loss within 1 min. Intermittent paradigms several seconds between stimulus trains also electrodes, never extinction after 3 min stimulation. Longer breaks each pulse train some recovery stimulus-evoked percepts. For had almost no perceived intensity.Intermittent more effective maintaining Given that transient neural activity dominates response during mechanical contact onsets offsets, providing brief these times may closely represent natural cortical have additional benefit prolonging ability evoke sensations

Язык: Английский

Процитировано

26