Nanotechnology in the life sciences, Год журнала: 2024, Номер unknown, С. 77 - 92
Опубликована: Янв. 1, 2024
Язык: Английский
Journal of Basic Microbiology, Год журнала: 2024, Номер 64(8)
Опубликована: Май 21, 2024
Abstract Entomopathogenic fungi are the most effective control remedy against a wide range of medical and agricultural important pests. The present study aimed to isolate, identify, assess virulence Metarhizium rileyi Spodoptera litura frugiperda pupae under soil conditions. biotechnological methods were used identify isolate as M. . fungal conidial pathogenicity (2.0 × 10 7 , 2.0 8 9 11 conidia/mL −1 ) was tested prepupae S. at 3, 6, 9, 12 days after treatments. Additionally, artificial soil‐conidial assay performed on nontarget species earthworm Eudrilus eugeniae using conidia. results showed that caused significant mortality rates in (61‐90%), more susceptible than (46%–73%) day posttreatment. LC 50 90 3.4 14 –9.9 17 6.6 5 –4.6 respectively. conidia did not exhibit any sublethal effect adult stage E. Artemia salina following 12‐day treatment period. Moreover, histopathological evaluation no discernible harm observed gut tissues including lumen epithelial cells, well muscles, setae, nucleus, mitochondria, coelom. findings provide clear evidence can be foundation for development bio‐insecticides combat
Язык: Английский
Процитировано
12
Environmental Quality Management, Год журнала: 2024, Номер 34(1)
Опубликована: Апрель 2, 2024
Abstract Chemical pesticides are synthetic substances created to manage plant pests. Frequent chemical usage causes extensive damage crops and contributes pest resistance, leading environmental contamination, harm non‐target organisms, the emergence of pesticide‐resistant species, potential health hazards for humans. The aim this study is isolate, identify, characterize biocontrol agents Trichoderma harzianum evaluate their effects on various phytopathogens. T. were tested against fungal phytopathogens, including Sclerotium rolfsii , Alternaria sp ., Pythium aphanidermatum Macrophomina phaseolina Fusarium oxysporum Helminthosporium sativum Aspergillus niger . results clearly showed that, compared other TVK1 demonstrated highest ability suppress with a growth inhibition rate 2.48 cm per day. TVK2 grows at 1.96 day, whereas TVK5 1.98 level antifungal volatile organic compounds produced by spores was higher pathogens species examined. This compound inhibits pathogens. can eliminate 100% S. Pathogens reduced 92.02% A. 94% F. P. 94.07% H. M. In detached leaf assay, inhibited in leaves, tomato, mustard, eggplant, when both commercially available fungicide control. confirms that isolate combat Early application reduce risk phytopathogenic attacks leaves promote growth.
Язык: Английский
Процитировано
10
Frontiers in Microbiology, Год журнала: 2024, Номер 15
Опубликована: Авг. 7, 2024
, known as the South American tomato leaf miner, significantly impacts plants (
Язык: Английский
Процитировано
8
Pesticide Biochemistry and Physiology, Год журнала: 2025, Номер 208, С. 106304 - 106304
Опубликована: Янв. 20, 2025
Язык: Английский
Процитировано
1
Heliyon, Год журнала: 2024, Номер 10(11), С. e31550 - e31550
Опубликована: Май 18, 2024
Agro-environmental sustainability is based upon the adoption of efficient resources in agro-practices that have a nominal impact on ecosystem. Insect pests are responsible for causing severe impacts crop productivity. Wide ranges agro-chemicals been employed over last 50 years to overcome yield losses due insect pests. But better knowledge about hazards chemical pesticides and other pest resistance resurgence issues necessitates an alternative control. The applications biological offer best alternate safe, cost-effective, easy successful against various pathogens. Like organisms, insects can get wide range diseases from microbes, such as bacteria, fungi, viruses, protozoa, nematodes. In order create agricultural management practices environmentally beneficial, bacterial entomopathogens being thoroughly studied. Utilization biopesticides has adopted protection products. different types toxin complexes released by microorganisms their mechanisms action recapitulated. present review described diversity biocontrol prospective certain bacteria summarised potential pests, insects, phytopathogenic practices.
Язык: Английский
Процитировано
6
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Фев. 28, 2025
The current study aimed to isolate Beauveria brongniartii conidia from forest soils, identify the fungus, and evaluate its effectiveness on eggs, larvae, pupae, adults of Spodoptera litura. Insect mortality rates were recorded every 3, 6, 9, 12 days. identification entomopathogenic fungi was carried out using molecular techniques, including PCR, DNA sequencing, markers, detect species-specific 18 S rDNA genetic sequences, all performed under aseptic conditions. results indicated that higher concentrations (2.7 × 109 conidia/mL) exhibited greater virulence, with eggs showing a rate 98.66%, followed by larvae 96%, 90.66%, pupae 77.33% after Probit analysis revealed minimal LC50 LC90 values: (5.5 102; 1.0 106 spores/mL), (8.2 1.2 107 (9.6 104; 7.3 1010 (1.0 103; 2.0 108 spores/mL). total hemocyte counts detailed observational B. induces cellular breakdown cell lysis in S. litura producing enzymes degrade cuticle membranes. Earthworm bioindicator studies showed effects compared controls, while chemical treatments resulted 96% at 100 ppm. Histopathological examinations no significant differences gut tissue between earthworms treated fungal those control group, unlike substantial damage caused treatments. Biochemical alterations enzyme activity, reduced levels phosphatase catalase, as well increased lipid peroxides superoxide dismutase. This highlights controlling litura, demonstrating potential viable biocontrol agent promoting eco-friendly alternatives pesticides, risk non-target species or environment.
Язык: Английский
Процитировано
0
Southwestern Entomologist, Год журнала: 2025, Номер 50(1)
Опубликована: Март 12, 2025
Язык: Английский
Процитировано
0
International Journal of Tropical Insect Science, Год журнала: 2025, Номер unknown
Опубликована: Март 27, 2025
Язык: Английский
Процитировано
0
Bulletin of Entomological Research, Год журнала: 2025, Номер unknown, С. 1 - 17
Опубликована: Апрель 24, 2025
Abstract Phycodes radiata is a significant pest of fig trees, causing extensive damage to orchards in the Semnan region Iran recent years. egg masses P. were collected from four Ficus carica cultivars, Zard Semnan, Ghermez Garmsar, and sp., subsequently reared under laboratory conditions. The study investigated biological parameters when fed on different cultivars. feeding index significantly higher cultivar than others. Hemocyte types observed larval hemolymph included prohemocytes, plasmatocytes, granulocytes, oenocytoids, as identified via light microscopy. pathogenicity five isolates entomopathogenic fungi: three Beauveria bassiana (B1, B2, B3), one isolate Metarhizium anisopliae, Lecanicillium muscarium assessed against larvae. larvae directly immersed fungal suspension for 6 seconds. Control treated similarly with Tween 80 (0.01%) distilled water. Larval mortality was highest B1, B3 isolates, respectively. To evaluate hemocyte density response pathogenic fungi, spores injected into insect hemolymph, type changes recorded at 1, 3, 6, 12, 24, 48, 72 hours post-injection. most pronounced effects B1 B2 3 Overall, findings indicate that fungi can effectively disrupt growth defense mechanisms radiata, leading impaired development increased mortality.
Язык: Английский
Процитировано
0
Frontiers in Microbiology, Год журнала: 2024, Номер 15
Опубликована: Окт. 8, 2024
This review provides a comprehensive analysis of the classification, biology, and management Drosophila species (Diptera: Drosophilidae) with focus on entomopathogenic fungi (EPF) as biocontrol strategy. species, particularly suzukii , melanogaster have emerged significant pests in various agricultural systems, causing extensive damage to fruit crops. Understanding their taxonomic classification biological traits is crucial for developing effective strategies. delves into life cycle, behavior, ecological interactions highlighting challenges posed by rapid reproduction adaptability. The further explores potential EPF an eco-friendly alternative chemical pesticides. mode action against examined, including spore adhesion, germination, penetration insect cuticle, leading host death. Factors influencing efficacy EPF, such environmental conditions, fungal virulence, specificity, are discussed detail. By synthesizing current research, this aims provide valuable insights application identify future research directions enhancing effectiveness EPF-based control measures species.
Язык: Английский
Процитировано
3