Mechanochemical Approach to Obtaining a Multicomponent Fisetin Delivery System Improving Its Solubility and Biological Activity
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(7), С. 3648 - 3648
Опубликована: Март 25, 2024
In
this
study,
binary
amorphous
solid
dispersions
(ASDs,
fisetin-Eudragit®)
and
ternary
inclusions
(ASIs,
fisetin-Eudragit®-HP-β-cyclodextrin)
of
fisetin
(FIS)
were
prepared
by
the
mechanochemical
method
without
solvent.
The
nature
FIS
in
ASDs
ASIs
was
confirmed
using
XRPD
(X-ray
powder
diffraction).
DSC
(Differential
scanning
calorimetry)
full
miscibility
multicomponent
delivery
systems.
FT-IR
(Fourier-transform
infrared
analysis)
interactions
that
stabilize
FIS's
state
identified
functional
groups
involved.
study
culminated
evaluating
impact
amorphization
on
water
solubility
conducting
vitro
antioxidant
assays:
2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic
acid)-ABTS,
2,2-diphenyl-1-picrylhydrazyl-DPPH,
Cupric
Reducing
Antioxidant
Capacity-CUPRAC,
Ferric
Power-FRAP
neuroprotective
inhibition
acetylcholinesterase-AChE
butyrylcholinesterase-BChE.
addition,
molecular
docking
allowed
for
determination
possible
bonds
between
mentioned
above
enzymes.
best
preparation
turned
out
to
be
ASI_30_EPO
(ASD
fisetin-Eudragit®
containing
30%
combination
with
HP-β-cyclodextrin),
which
showed
an
improvement
apparent
(126.5
±
0.1
µg∙mL-1)
properties
(ABTS:
IC50
=
10.25
µg∙mL-1,
DPPH:
27.69
CUPRAC:
IC0.5
9.52
FRAP:
8.56
(inhibition
AChE:
39.91%,
BChE:
42.62%).
Язык: Английский
The Potential Role of Adipose-Derived Stem Cells in Regeneration of Peripheral Nerves
Neurology International,
Год журнала:
2025,
Номер
17(2), С. 23 - 23
Опубликована: Фев. 6, 2025
Peripheral
nerve
injuries
are
common
complications
in
surgical
and
dental
practices,
often
resulting
functional
deficiencies
reduced
quality
of
life.
Current
treatment
choices,
such
as
autografts,
have
limitations,
including
donor
site
morbidity
suboptimal
outcomes.
Adipose-derived
stem
cells
(ADSCs)
shown
assuring
regenerative
potential
due
to
their
accessibility,
ease
harvesting
propagation,
multipotent
properties.
This
review
investigates
the
therapeutic
ADSCs
peripheral
regeneration,
focusing
on
use
bioengineered
conduits
supportive
microenvironments.
The
analysis
is
constructed
published
case
reports,
organized
reviews,
clinical
trials
from
Phase
I
III
that
investigate
managing
injuries,
emphasizing
both
orofacial
applications.
findings
highlight
advantages
promoting
secretion
angiogenic
neurotrophic
factors,
support
for
cellular
persistence,
supplementing
scaffold-based
tissue
repair.
capabilities
offer
a
novel
approach
augmenting
repair
recovery.
accessibility
adipose
minimally
invasive
nature
ADSC
further
encourage
its
prospective
application
an
autologous
cell
source
medicine.
Future
research
needed
ascertain
standardized
protocols
optimize
outcomes,
paving
way
become
mainstay
regeneration.
Язык: Английский
Optimally Aligned Nerve Scaffolds with Sustained Astaxanthin Release Enhance the Inflammatory Microenvironment Via Mitophagy Activation
Опубликована: Янв. 1, 2025
Язык: Английский
Bridging the Gap: Contemporary Paradigms in Nerve Scaffolds and Regeneration
Advances in Wound Care,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 11, 2025
Peripheral
nerve
injuries,
especially
those
with
complete
transection
of
major
nerves,
create
significant
morbidity
including
debilitating
pain,
loss
protective
haptic
feedback,
and
impaired
volitional
control
musculature.
The
societal
burden
cost
medical
care
for
these
injuries
are
enormous,
estimates
in
the
United
States
alone
excess
$670
million
per
year.
In
clinical
scenarios
a
segmental
gap
where
end-to-end
coaptation
without
tension
is
not
possible,
"bridge"
or
scaffold
must
be
interposed
to
facilitate
communication
between
proximal
distal
stumps
organized
growth
following
Wallerian
degeneration.
A
multitude
constructs
have
been
created
studied
this
regeneration.
Among
three
overall
types
bridge
employed
contemporary
care-conduit/scaffold,
allograft,
autograft-each
has
downsides
ranging
from
limited
successful
ingrowth
donor
site
morbidity.
Despite
tremendous
work
over
last
150
years
biology
technology
treatment
peripheral
injury,
biological
processes
governing
regeneration
remain
incompletely
understood.
Especially
cases
long
gaps,
there
remains
room
improvement.
Ongoing
studies
identified
several
promising
modalities
scaffolds
more
efficient
effective
neuronal
outgrowth
but
still
require
further
investigation.
Here,
we
review
paradigms
interposing
reexamine
physiology,
regulatory
programs
regeneration,
strategic
targets
neurogenic
pathways
that
may
novel
modalities.
Язык: Английский
Optimally Aligned Nerve Scaffolds with Sustained Astaxanthin Release Improve the Inflammatory Microenvironment through Mitophagy Activation
Small,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 15, 2025
Abstract
Effective
repair
of
peripheral
nerve
injury
(PNI)
depends
on
the
scaffold
orientation
and
immunomodulatory
capabilities
functionalized
scaffolds,
both
which
substantially
influence
regeneration.
In
this
study,
composite
scaffolds
incorporating
astaxanthin
(AXT)
polycaprolactone
(PCL)
are
developed
to
investigate
blend
concentration
cellular
behavior,
including
adhesion,
migration,
proliferation.
vitro
analysis
identifies
0.2%
AXT/PCL
fabricated
at
a
rotational
speed
400
rpm
as
optimal
configuration
for
facilitating
directed
cell
growth
guiding
repair.
Moreover,
controlled
release
AXT
improves
microenvironment
by
preserving
mitochondrial
homeostasis,
promoting
mitophagy,
reducing
oxidative
stress
inflammation.
vivo
assessments
reveal
that
group
(0.2%
AXT/PCL‐400)
achieves
better
morphological,
histological,
electrophysiological,
functional
recovery
than
PCL,
AXT/PCL+M0,
AXT/PCL+M4
groups,
approaching
outcomes
observed
in
autograft
(Auto)
group.
demonstrates
regenerative
PCL
AXT/PCL+M0
underscoring
critical
role
mitophagy
regulating
microenvironment.
Язык: Английский
Therapeutic Potential and Challenges of Mesenchymal Stem Cell-Derived Exosomes for Peripheral Nerve Regeneration: A Systematic Review
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(12), С. 6489 - 6489
Опубликована: Июнь 12, 2024
Gap
injuries
to
the
peripheral
nervous
system
result
in
pain
and
loss
of
function,
without
any
particularly
effective
therapeutic
options.
Within
this
context,
mesenchymal
stem
cell
(MSC)-derived
exosomes
have
emerged
as
a
potential
option.
Thus,
focus
study
was
review
currently
available
data
on
MSC-derived
exosome-mounted
scaffolds
nerve
regeneration
order
identify
most
promising
exosome
sources
field
regeneration.
We
conducted
systematic
following
PRISMA
2020
guidelines.
Exosome
origins
varied
(adipose-derived
MSCs,
bone
marrow
gingival
MSC,
induced
pluripotent
cells
purified
product)
similarly
materials
(Matrigel,
alginate
silicone,
acellular
graft
[ANG],
chitosan,
chitin,
hydrogel
fibrin
glue).
The
compound
muscle
action
(CMAP),
sciatic
functional
index
(SFI),
gastrocnemius
wet
weight
histological
analyses
were
used
main
outcome
measures.
Overall,
showed
better
than
alone.
Functionally,
both
exosome-enriched
chitin
ANG
significant
improvement
over
time
sciatica
index,
CMAP
weight.
best
outcomes
found
scaffold
with
high
increase
axonal
diameter
cross-section
area.
Further
studies
are
needed
confirm
efficacy
Язык: Английский
Application and progress of bionic scaffolds in nerve repair: a narrative review
Advanced technology in neuroscience .,
Год журнала:
2024,
Номер
1(1), С. 43 - 50
Опубликована: Сен. 1, 2024
Nerve
injury
can
result
in
severe
damage
and
potentially
permanent
disability,
imposing
substantial
physical,
psychological,
economic
burdens
on
affected
individuals
their
families.
Despite
advances
surgical
repair
techniques,
the
functional
recovery
of
nerves
remains
suboptimal.
The
current
therapeutic
approaches
for
nerve
exhibit
limited
efficacy
restoring
function,
underscoring
imperative
development
innovative
treatment
modalities.
In
recent
years,
bionics
has
emerged
as
a
promising
field
medicine,
particularly
rehabilitation
injuries.
We
review
application
bionic
technology
within
realm
treatment,
encompassing
scaffolds,
regeneration
materials,
modulation
techniques.
delve
into
how
these
technologies
may
facilitate
restoration
tissues,
well
challenges
they
encounter
clinical
translation
prospective
directions
future
development.
Furthermore,
we
explore
convergence
with
existing
strategies
discuss
potential
interdisciplinary
collaboration
to
catalyze
innovation
treatment.
integration
conventional
methods
offer
synergistic
approach,
enhancing
processes.
Язык: Английский
Mechanism and Application of Biomaterials Targeting Reactive Oxygen Species and Macrophages in Inflammation
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
26(1), С. 245 - 245
Опубликована: Дек. 30, 2024
Inflammation,
an
adaptive
reaction
to
harmful
stimuli,
is
a
necessary
immune
system
response
and
can
be
either
acute
or
chronic.
Since
inflammation
tends
eliminate
stimuli
restore
equilibrium,
it
generally
advantageous
the
organism.
Chronic
inflammation,
however,
caused
by
increased
inflammatory
signaling
decreased
pro-anti-inflammatory
signaling.
According
current
studies,
thought
major
factor
in
number
of
chronic
diseases,
including
diabetes,
cancer,
arthritis,
bowel
disease,
obesity.
Consequently,
reducing
essential
for
both
preventing
delaying
diseases.
The
application
biomaterials
treatment
illnesses
has
grown
recent
years.
A
variety
implanted
themselves
conjunction
with
other
bioactive
ingredients
therapeutic
agents.
mechanisms
action
applications
well-known
anti-inflammatory
are
main
topics
this
article.
Язык: Английский