A novel antibacterial hydrogel containing aminophylline as a versatile platform for neural differentiation of hWJMSCs through the CREB pathway DOI Creative Commons

Akram Sadat Seyedi Jasbi,

Hossein Aghamollaei, Zohreh Jangravi

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Май 24, 2025

This study aims to develop a novel antibacterial hydrogel scaffold composed of gelatin (Gel), amniotic membrane extract (AME), and aminophylline (AMP) for neural regeneration. We investigate its ability sustain AMP release, inhibit bacterial growth, promote differentiation human Wharton's jelly mesenchymal stem cells (hWJMSCs) via the CREB pathway, addressing unmet needs in tissue engineering. The composite hydrogels were synthesized characterized using various methods techniques, including X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), porosity, contact angle, water uptake, thermogravimetric analysis (TGA), biodegradation, tensile strength, drug activity. Biocompatibility tests (MTT assay, AO/EB staining) confirmed > 95% viability hWJMSCs over six days their was analyzed through immunocytochemistry (ICC) staining real-time reverse transcription-polymerase chain reaction (RT-PCR) at different time points. results demonstrate successful synthesis porous with desirable properties, hydrophilicity, thermal stability, biodegradability, mechanical strength. support sustained release (53.18% 336 h) exhibit activity against Pseudomonas aeruginosa (90.52 ± 0.26%) Staphylococcus aureus (93.06 0.34%) due presence penicillin streptomycin (P-S) antibiotics. biocompatibility show that do not have cytotoxic effect on WJMSCs. WJMSCs seeded surface by evaluating specific markers both protein gene levels. In conclusion, new gel-based can after further evaluation, be introduced as candidate repair applications.

Язык: Английский

Liquid Metal‐Based Conductive Nerve Guidance Conduit Combined With Electrical Stimulation Boosts Peripheral Nerve Repair DOI Open Access
Yujie Zhu, Chenchen Song, Dongdong Yao

и другие.

Journal of Biomedical Materials Research Part A, Год журнала: 2025, Номер 113(2)

Опубликована: Фев. 1, 2025

The combination of nerve guide conduits (NGCs) and electrical stimulation (ES) is an effective treatment for peripheral injury (PNI). Flexible conductive materials with mechanical properties similar to those biological tissues have been shown better long-term biointegration functionality than rigid materials. In this study, liquid metal (LM)-based polycaprolactone/gelatin/polypyrrole/LM (PCL/Gel/PPy/LM, PGPL) NGC was combined exogenous ES repair PNI. PGPL membranes had good hydrophilicity, degradability, properties, its conductivity reached 0.66 ± 0.02 S/m. vitro studies showed that the (2 Hz, 100 mV/cm, 30 min/d) could significantly increase expression neuromarkers a pro-neural differentiation effect. vivo demonstrated NGCs in 200 mV/mm, effectively promote morphological reconstruction functional recovery sciatic rats. At 3 months post-surgery, restored conduction velocity 73.85% 5.45% normal value. LM-based prepared study long defects, which may further expand application LM field tissue engineering.

Язык: Английский

Процитировано

2
A novel antibacterial hydrogel containing aminophylline as a versatile platform for neural differentiation of hWJMSCs through the CREB pathway DOI Creative Commons

Akram Sadat Seyedi Jasbi,

Hossein Aghamollaei, Zohreh Jangravi

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Май 24, 2025

This study aims to develop a novel antibacterial hydrogel scaffold composed of gelatin (Gel), amniotic membrane extract (AME), and aminophylline (AMP) for neural regeneration. We investigate its ability sustain AMP release, inhibit bacterial growth, promote differentiation human Wharton's jelly mesenchymal stem cells (hWJMSCs) via the CREB pathway, addressing unmet needs in tissue engineering. The composite hydrogels were synthesized characterized using various methods techniques, including X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), porosity, contact angle, water uptake, thermogravimetric analysis (TGA), biodegradation, tensile strength, drug activity. Biocompatibility tests (MTT assay, AO/EB staining) confirmed > 95% viability hWJMSCs over six days their was analyzed through immunocytochemistry (ICC) staining real-time reverse transcription-polymerase chain reaction (RT-PCR) at different time points. results demonstrate successful synthesis porous with desirable properties, hydrophilicity, thermal stability, biodegradability, mechanical strength. support sustained release (53.18% 336 h) exhibit activity against Pseudomonas aeruginosa (90.52 ± 0.26%) Staphylococcus aureus (93.06 0.34%) due presence penicillin streptomycin (P-S) antibiotics. biocompatibility show that do not have cytotoxic effect on WJMSCs. WJMSCs seeded surface by evaluating specific markers both protein gene levels. In conclusion, new gel-based can after further evaluation, be introduced as candidate repair applications.

Язык: Английский

Процитировано

0