Life,
Год журнала:
2025,
Номер
15(4), С. 655 - 655
Опубликована: Апрель 16, 2025
Protein
kinase
A
(PKA)
is
a
key
regulator
of
cellular
signaling
that
regulates
physiological
processes
such
as
metabolism,
cell
proliferation,
and
neuronal
function.
While
its
activation
by
the
second
messenger
3′,5′-cyclic
adenosine
triphosphate
(cAMP)
well
characterized,
recent
research
highlights
additional
regulatory
mechanisms,
particularly
oxidative
post-translational
modifications,
influence
PKA’s
structure,
activity,
substrate
specificity.
Both
catalytic
subunits
PKA
are
susceptible
to
redox
which
have
been
shown
play
important
roles
in
regulation
functions,
including
cardiac
contractility,
lipid
immune
response.
Likewise,
redox-dependent
modulation
has
implicated
numerous
diseases,
cardiovascular
disorders,
diabetes,
neurodegenerative
conditions,
making
it
potential
therapeutic
target.
However,
mechanisms
crosstalk
between
redox-
PKA-dependent
remain
poorly
understood.
This
review
examines
structural
functional
PKA,
with
focus
on
modifications
their
impact
signaling.
deeper
understanding
these
may
provide
new
strategies
for
targeting
stress
disease
restoring
balanced
cells.
PeerJ,
Год журнала:
2025,
Номер
13, С. e18950 - e18950
Опубликована: Март 20, 2025
Introduction
Phosphoproteomics,
an
advanced
branch
of
molecular
biology,
utilizes
specific
techniques
such
as
mass
spectrometry,
affinity
chromatography,
and
bioinformatics
analysis
to
explore
protein
phosphorylation,
shedding
light
on
the
cellular
mechanisms
that
drive
various
biological
processes.
This
field
has
become
instrumental
in
advancing
our
understanding
renal
diseases,
from
identifying
underlying
pinpointing
new
therapeutic
targets.
Areas
covered
review
will
discuss
evolution
phosphoproteomics
its
early
experimental
observations
current
application
disease
research
using
liquid
chromatography-tandem
spectrometry
(LC-MS/MS).
We
role
identification
biomarkers,
elucidation
pathogenic
mechanisms,
development
novel
strategies.
Additionally,
potential
enhancing
drug
discovery
improving
treatment
outcomes
for
diseases
be
highlighted.
Expert
opinion
Phosphoproteomics
is
rapidly
transforming
by
offering
unprecedented
insights
into
Utilizing
LC-MS/MS,
it
enables
biomarkers
targets,
mechanisms.
promises
significant
advancements
diagnosis
shifting
towards
more
personalized
effective
As
technology
evolves,
integration
clinical
practice
pivotal
revolutionizing
healthcare.
Poultry Science,
Год журнала:
2025,
Номер
unknown, С. 105091 - 105091
Опубликована: Март 1, 2025
Salmonella
infection
severely
impacts
poultry
health,
productivity,
and
food
safety.
Spray
dried
plasma
(SDP)
shows
good
efficacy
as
an
alternative
to
in-feed
antibiotics
for
broiler
chickens.
Our
objective
was
identify
potential
mechanisms
of
SDP
benefits
by
analyzing
effects
on
spleen
proteome
in
response
Enteritidis
challenge
(SE).
A
2
×
factor
study
broilers
(n
=
240)
were
assigned
CON
(control)
or
(30g/kg)
diet
at
1
day
age,
gavaged
with
sterile
tryptic
soy
7.46
108
CFU
SE
/mL,
resulting
4
groups
60),
CON-SE
60)
SDP-SE
60).
On
d
5
post-challenge,
chicks
4/treatment)
euthanized,
collected
isolate
proteins,
measure
using
label-free
liquid
chromatography
tandem
mass
spectrometry.
Data
mapped
Gallus
gallus
Uniprot
database,
differentially
abundant
proteins
(DAP;
P
<
0.05)
identified
ANOVA.
Across
all
samples,
4,300
identified,
treatment
resulted
116
DAP.
Proteins
(56)
that
decreased
associated
positive
regulation
I-kappaß
kinase/NF-
kappaß
signaling
apoptosis
process.
increased
(60)
integral
components
endoplasmic
reticulum
membrane
necroptosis
pathway.
Analysis
the
interaction
between
found
119
The
(87)
RNA
binding,
ATP
mRNA
splicing,
protein
phosphorylation.
Proteomic
signatures
reveals
actions
are
anti-inflammatory
anti-apoptotic
predicted
inhibit
pro-inflammatory
through
inhibition
NF-ĸB.
However,
presence
infection,
it
promotes
regulated
inflammatory
response.
Life,
Год журнала:
2025,
Номер
15(4), С. 655 - 655
Опубликована: Апрель 16, 2025
Protein
kinase
A
(PKA)
is
a
key
regulator
of
cellular
signaling
that
regulates
physiological
processes
such
as
metabolism,
cell
proliferation,
and
neuronal
function.
While
its
activation
by
the
second
messenger
3′,5′-cyclic
adenosine
triphosphate
(cAMP)
well
characterized,
recent
research
highlights
additional
regulatory
mechanisms,
particularly
oxidative
post-translational
modifications,
influence
PKA’s
structure,
activity,
substrate
specificity.
Both
catalytic
subunits
PKA
are
susceptible
to
redox
which
have
been
shown
play
important
roles
in
regulation
functions,
including
cardiac
contractility,
lipid
immune
response.
Likewise,
redox-dependent
modulation
has
implicated
numerous
diseases,
cardiovascular
disorders,
diabetes,
neurodegenerative
conditions,
making
it
potential
therapeutic
target.
However,
mechanisms
crosstalk
between
redox-
PKA-dependent
remain
poorly
understood.
This
review
examines
structural
functional
PKA,
with
focus
on
modifications
their
impact
signaling.
deeper
understanding
these
may
provide
new
strategies
for
targeting
stress
disease
restoring
balanced
cells.
