Redox Regulation of cAMP-Dependent Protein Kinase and Its Role in Health and Disease DOI Creative Commons

Ese Ekhator,

Marco Fazzari, Robert H. Newman

et al.

Life, Journal Year: 2025, Volume and Issue: 15(4), P. 655 - 655

Published: April 16, 2025

Protein kinase A (PKA) is a key regulator of cellular signaling that regulates physiological processes such as metabolism, cell proliferation, and neuronal function. While its activation by the second messenger 3′,5′-cyclic adenosine triphosphate (cAMP) well characterized, recent research highlights additional regulatory mechanisms, particularly oxidative post-translational modifications, influence PKA’s structure, activity, substrate specificity. Both catalytic subunits PKA are susceptible to redox which have been shown play important roles in regulation functions, including cardiac contractility, lipid immune response. Likewise, redox-dependent modulation has implicated numerous diseases, cardiovascular disorders, diabetes, neurodegenerative conditions, making it potential therapeutic target. However, mechanisms crosstalk between redox- PKA-dependent remain poorly understood. This review examines structural functional PKA, with focus on modifications their impact signaling. deeper understanding these may provide new strategies for targeting stress disease restoring balanced cells.

Language: Английский

Applications and prospects of phosphoproteomics in renal disease research DOI Creative Commons

Xuejia Zheng,

Lingling Zhou, Tiantian Xu

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18950 - e18950

Published: March 20, 2025

Introduction Phosphoproteomics, an advanced branch of molecular biology, utilizes specific techniques such as mass spectrometry, affinity chromatography, and bioinformatics analysis to explore protein phosphorylation, shedding light on the cellular mechanisms that drive various biological processes. This field has become instrumental in advancing our understanding renal diseases, from identifying underlying pinpointing new therapeutic targets. Areas covered review will discuss evolution phosphoproteomics its early experimental observations current application disease research using liquid chromatography-tandem spectrometry (LC-MS/MS). We role identification biomarkers, elucidation pathogenic mechanisms, development novel strategies. Additionally, potential enhancing drug discovery improving treatment outcomes for diseases be highlighted. Expert opinion Phosphoproteomics is rapidly transforming by offering unprecedented insights into Utilizing LC-MS/MS, it enables biomarkers targets, mechanisms. promises significant advancements diagnosis shifting towards more personalized effective As technology evolves, integration clinical practice pivotal revolutionizing healthcare.

Language: Английский

Citations

0
Spleen proteome profile reveals immune responses induced by dietary spray-dried plasma against Salmonella Enteritidis infection in broiler chicks DOI Creative Commons
Tunde E. Ogundare, Theresa Casey, Uma K. Aryal

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105091 - 105091

Published: March 1, 2025

Salmonella infection severely impacts poultry health, productivity, and food safety. Spray dried plasma (SDP) shows good efficacy as an alternative to in-feed antibiotics for broiler chickens. Our objective was identify potential mechanisms of SDP benefits by analyzing effects on spleen proteome in response Enteritidis challenge (SE). A 2 × factor study broilers (n = 240) were assigned CON (control) or (30g/kg) diet at 1 day age, gavaged with sterile tryptic soy 7.46 108 CFU SE /mL, resulting 4 groups 60), CON-SE 60) SDP-SE 60). On d 5 post-challenge, chicks 4/treatment) euthanized, collected isolate proteins, measure using label-free liquid chromatography tandem mass spectrometry. Data mapped Gallus gallus Uniprot database, differentially abundant proteins (DAP; P < 0.05) identified ANOVA. Across all samples, 4,300 identified, treatment resulted 116 DAP. Proteins (56) that decreased associated positive regulation I-kappaß kinase/NF- kappaß signaling apoptosis process. increased (60) integral components endoplasmic reticulum membrane necroptosis pathway. Analysis the interaction between found 119 The (87) RNA binding, ATP mRNA splicing, protein phosphorylation. Proteomic signatures reveals actions are anti-inflammatory anti-apoptotic predicted inhibit pro-inflammatory through inhibition NF-ĸB. However, presence infection, it promotes regulated inflammatory response.

Language: Английский

Citations

0
Deep Learning Techniques Applied to Phosphorylation Site Prediction: A Systematic Review DOI
Antônio Carlos da Silva Júnior, André Massahiro Shimaoka, Luciano Rodrigo Lopes

et al.

SN Computer Science, Journal Year: 2025, Volume and Issue: 6(4)

Published: April 4, 2025

Language: Английский

Citations

0
Controlling pyroptosis through post-translational modifications of gasdermin D DOI
Na Zhang, Daichao Xu

Developmental Cell, Journal Year: 2025, Volume and Issue: 60(7), P. 994 - 1007

Published: April 1, 2025

Language: Английский

Citations

0
Redox Regulation of cAMP-Dependent Protein Kinase and Its Role in Health and Disease DOI Creative Commons

Ese Ekhator,

Marco Fazzari, Robert H. Newman

et al.

Life, Journal Year: 2025, Volume and Issue: 15(4), P. 655 - 655

Published: April 16, 2025

Protein kinase A (PKA) is a key regulator of cellular signaling that regulates physiological processes such as metabolism, cell proliferation, and neuronal function. While its activation by the second messenger 3′,5′-cyclic adenosine triphosphate (cAMP) well characterized, recent research highlights additional regulatory mechanisms, particularly oxidative post-translational modifications, influence PKA’s structure, activity, substrate specificity. Both catalytic subunits PKA are susceptible to redox which have been shown play important roles in regulation functions, including cardiac contractility, lipid immune response. Likewise, redox-dependent modulation has implicated numerous diseases, cardiovascular disorders, diabetes, neurodegenerative conditions, making it potential therapeutic target. However, mechanisms crosstalk between redox- PKA-dependent remain poorly understood. This review examines structural functional PKA, with focus on modifications their impact signaling. deeper understanding these may provide new strategies for targeting stress disease restoring balanced cells.

Language: Английский

Citations

0