International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(18), С. 9778 - 9778
Опубликована: Сен. 10, 2024
Like
most
tumors,
glioblastoma
multiforme
(GBM),
the
deadliest
brain
tumor
in
human
adulthood,
releases
extracellular
vesicles
(EVs).
Their
content,
reflecting
that
of
origin,
can
be
donated
to
nearby
and
distant
cells
which,
by
acquiring
it,
become
more
aggressive.
Therefore,
study
EV-transported
molecules
has
very
important.
Particular
attention
been
paid
EV
proteins
uncover
new
GBM
biomarkers
potential
druggable
targets.
Proteomic
studies
have
mainly
performed
“bottom-up”
mass
spectrometry
(MS)
analysis
EVs
isolated
different
procedures
from
conditioned
media
cultured
biological
fluids
patients.
Although
a
great
number
dysregulated
identified,
translation
these
findings
into
clinics
remains
elusive,
probably
due
multiple
factors,
including
lack
standardized
for
isolation/characterization
their
proteome.
Thus,
it
is
time
change
research
strategies
adopting,
addition
harmonized
selection
techniques,
MS
methods
aimed
at
identifying
selected
tumoral
protein
mutations
and/or
isoforms
post-translational
modifications,
which
deeply
influence
behavior.
Hopefully,
data
integrated
with
those
other
“omics”
disciplines
will
lead
discovery
pathways
novel
therapies.
Microchimica Acta,
Год журнала:
2025,
Номер
192(4)
Опубликована: Март 5, 2025
The
review
article
provides
a
short
introduction
to
exosomes
with
the
focus
use
as
disease
markers
itself
(i.e.
their
concentration
or
presence
of
some
specific
receptors)
source
biomarkers
such
proteins
and
metabolites.
In
detail,
we
are
discussing
various
methods
exosome
isolation
main
paper
is
on
affinity
capture
exosomes,
since
them
can
be
applied
sub-populations
produced
by
organs.
comprehensive
overview
magnetic
(bio)affinity
detection
exosomal
cargo
using
different
ligands
antibodies,
DNA
aptamers,
peptides,
glycan-based
recognition,
transferrin-based
approaches,
based
recognition
phospholipids
other
approaches
including
electrostatic
interactions.
in
detail
key
analytical
clinical
parameters
form
an
extensive
table
summarising
outcomes
published
last
two
years
(2023-2024).
Finally,
also
conclusions
sections
pros
cons
for
and/or
determination
content.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 17, 2024
Abstract
Extracellular
vesicles
(EVs)
are
now
being
increasingly
recognized
as
an
essential
signaling
entity
in
human
plasma,
linking
them
to
health
and
various
diseases.
Still,
their
core
protein
lipid
componentry,
which
lie
at
the
center
of
EV
form
function,
remains
poorly
defined.
Achieving
this
unmet
milestone
greatly
hindered
by
abundant
non-vesicular
extracellular
plasma
components
(non-EVs)
mass
spectrometry-based
analyses.
Here,
we
performed
high-resolution
density
gradient
fractionation
over
110
samples
isolate
circulating
EVs,
systematically
construct
quantitative
proteome
(4500
proteins)
lipidome
(829
lipids)
landscapes.
This
led
discovery
a
highly
conserved
panel
182
proteins
(ADAM10,
STEAP23,
STX7)
52
lipids
(PS,
PIPs,
Hex2Cer,
PAs),
providing
deep
survey
hallmark
molecular
features
biological
pathways
intrinsic
EVs.
Our
efforts
also
mapped
surfaceome
diversity,
identifying
151
on
surface.
We
further
establish
set
42
114
that
served
non-EV
particles
plasma.
submit
ADAM10
PS(36:1)
markers
precisely
differentiates
between
particles.
findings,
can
be
explored
via
open-source
Shiny
web
tool
(
evmap.shinyapps.io/evmap/
)
will
serve
valuable
repository
research
community
for
clearer
understanding
biology.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 25, 2025
Mass
spectrometry-based
proteomics
is
about
35
years
old,
and
recent
progress
appears
to
be
speeding
up
across
all
subfields.
In
this
review,
we
focus
on
advances
over
the
last
two
in
select
areas
within
bottom-up
proteomics,
including
approaches
high-throughput
experiments,
data
analysis
using
machine
learning,
drug
discovery,
glycoproteomics,
extracellular
vesicle
structural
proteomics.
Proteomes,
Год журнала:
2025,
Номер
13(1), С. 12 - 12
Опубликована: Март 4, 2025
Extracellular
vesicles
(EVs)
are
lipid-bound
secreted
by
cells,
including
exosomes,
microvesicles,
and
apoptotic
bodies.
Proteomic
analyses
of
EVs,
particularly
in
relation
to
cancer,
reveal
specific
biomarkers
crucial
for
diagnosis
therapy.
However,
isolation
techniques
such
as
ultracentrifugation,
size-exclusion
chromatography,
ultrafiltration
face
challenges
regarding
purity,
contamination,
yield.
Contamination
from
other
proteins
complicates
downstream
processing,
leading
difficulties
identifying
interpreting
results.
Future
research
will
focus
on
refining
EV
characterization
diagnostic
therapeutic
applications,
improving
proteomics
tools
greater
accuracy,
exploring
the
use
EVs
drug
delivery
regenerative
medicine.
In
this
review,
we
provide
a
bird’s
eye
view
various
challenges,
starting
with
methods,
yield,
limitations
proteome
analysis
protein
targets.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 25, 2025
Extracellular
vesicles
(EVs)
play
crucial
roles
in
intercellular
communication
and
hold
great
promise
as
biomarkers
for
noninvasive
disease
diagnosis.
Intensive
research
efforts
have
been
devoted
to
discovering
the
EV
subpopulations
responsible
specific
functions
or
with
enhanced
effectiveness
markers,
through
extensive
purification
content
analysis.
However,
their
high
heterogeneity
size
cargo
composition
poses
significant
challenges
reaching
such
goals.
Isolation
methods
like
ultracentrifugation
size-exclusion
chromatography,
well
analysis
approaches
polymerase
chain
reaction
enzyme-linked
immunosorbent
assay,
made
contributions
improving
our
understanding
of
biology.
Nonetheless,
these
face
limitations
isolation
efficiency,
purity,
detection
sensitivity
specificity
due
issues
large
sample
consumption,
unsatisfactory
insufficient
resolution
subtyping.
Microfluidic
technology
presents
promising
solutions
challenges,
leveraging
intrinsic
capabilities
precise
flow
external
energy
field
manipulation,
compartmentalization,
signal
enhancement
at
micro-
nanoscale.
Hence,
this
review
summarizes
recent
developments
microfluidics-enabled
analysis,
paying
special
attention
unique
microfluidic
features
exploited.
Strategies
viscoelastic
inertial
flow,
fluid
mixing,
external-field-assisted
purification,
compartmentalization
micro/nanostructures
enhancing
detection,
are
examined.
Furthermore,
current
potential
future
directions
discussed
inspire
advancements
rapidly
developing
field.
Clinical Science,
Год журнала:
2024,
Номер
138(15), С. 941 - 962
Опубликована: Июль 17, 2024
Abstract
Atrial
fibrillation
(AF)
remains
challenging
to
prevent
and
treat.
A
key
feature
of
AF
is
atrial
enlargement.
However,
not
all
enlargement
progresses
AF.
in
response
physiological
stimuli
such
as
exercise
typically
benign
reversible.
Understanding
the
differences
function
molecular
profile
underpinning
pathological
remodelling
will
be
critical
for
identifying
new
strategies
The
discovery
mechanisms
responsible
ventricular
hypertrophy
has
uncovered
drug
targets
heart
failure.
Studies
atria
have
been
limited
comparison.
Here,
we
characterised
mouse
from
(1)
a
model
(cardiomyocyte-specific
transgenic
(Tg)
that
develops
dilated
cardiomyopathy
[DCM]
due
reduced
protective
signalling
[PI3K];
DCM-dnPI3K),
(2)
Tg
with
an
enlarged
increased
insulin-like
growth
factor
1
receptor;
IGF1R).
Both
models
presented
increase
mass,
but
displayed
distinct
functional,
cellular,
histological
phenotypes.
DCM-dnPI3K
Tg,
IGF1R
was
associated
dysfunction,
fibrosis
failure
gene
expression
pattern.
proteomics
identified
protein
networks
related
cardiac
contractility,
sarcomere
assembly,
metabolism,
mitochondria,
extracellular
matrix
which
were
differentially
regulated
models;
many
co-identified
data
sets
human
In
summary,
are
features,
proteomic
dataset
provides
resource
study
potential
regulators
biology
function,
biomarkers
Abstract
The
ability
of
trophectodermal
cells
(outer
layer
the
embryo)
to
attach
endometrial
and
subsequently
invade
underlying
matrix
are
critical
stages
embryo
implantation
during
successful
pregnancy
establishment.
Extracellular
vesicles
(EVs)
have
been
implicated
in
embryo‐maternal
crosstalk,
capable
reprogramming
towards
a
pro‐implantation
signature
phenotype.
However,
challenges
associated
with
EV
yield
direct
loading
biomolecules
limit
their
therapeutic
potential.
We
previously
established
generation
cell‐derived
nanovesicles
(NVs)
from
human
(hTSCs)
capacity
reprogram
enhance
adhesion
blastocyst
outgrowth.
Here,
we
employed
rapid
NV
strategy
encapsulate
potent
molecules
such
as
HB‐EGF
(NV
HBEGF
).
show
these
loaded
NVs
elicit
EGFR‐mediated
effects
recipient
cells,
activating
kinase
phosphorylation
sites
that
modulate
activity
(AKT
S124/129,
MAPK1
T185/Y187),
downstream
signalling
pathways
processes
signal
transduction,
GTPase
activity).
Importantly,
they
enhanced
target
cell
attachment
invasion.
phosphoproteomics
proteomics
approach
highlight
‐mediated
short‐term
patterns
long‐term
capabilities
on
which
functionally
trophectodermal‐endometrial
interactions.
This
proof‐of‐concept
study
demonstrates
feasibility
enhancing
functional
potency
context
implantation.
