First transcriptome of the Neotropical pest Euschistus heros (Hemiptera: Pentatomidae) with dissection of its siRNA machinery DOI Creative Commons
Deise Cagliari, Naymã Pinto Dias, Ericmar Ávila dos Santos

и другие.

Scientific Reports, Год журнала: 2020, Номер 10(1)

Опубликована: Март 17, 2020

Abstract Over the past few years, use of RNA interference (RNAi) for insect pest management has attracted considerable interest in academia and industry as a pest-specific environment-friendly strategy control. For success this technique, presence core RNAi genes functional silencing machinery is essential. Therefore, aim study was to test whether Neotropical brown stinkbug Euschistus heros main supply dsRNA could generate an efficient gene response. To do this, total mRNA all developmental stages sequenced on Illumina platform, followed by de novo assembly, annotation RNAi-related identification. Once were identified, nuclease activities hemolymph investigated through ex vivo assay. functionality siRNA machinery, E. adults microinjected with ~28 ng per mg targeting V-ATPase-A gene. Mortality, relative transcript levels , expression involved Dicer-2 ( DCR-2 ) Argonaute 2 AGO-2 ), analyzed. Transcriptome sequencing generated more than 126 million reads, these annotated approximately 80,000 contigs. The search resulted 47 three major pathways, absence sid-like homologous. Although incubation showed rapid degradation, there 35% mortality at 4 days after treatment significant reduction expression. These results indicated that although s id-like are lacking, uptake mechanism very efficient. Also, 2-fold 4-fold overexpression respectively, activation machinery. Consequently, proven be sensitive upon injection into its hemocoel. We believe finding together publically available transcriptome validation responsive provide starting point future field applications against one most important soybean pests South America.

Язык: Английский

Ecology, Worldwide Spread, and Management of the Invasive South American Tomato Pinworm, Tuta absoluta: Past, Present, and Future DOI
Antonio Biondi, R. N. C. Guedes,

Fang-Hao Wan

и другие.

Annual Review of Entomology, Год журнала: 2017, Номер 63(1), С. 239 - 258

Опубликована: Окт. 4, 2017

The South American tomato pinworm, Tuta absoluta (Meyrick), is native to the western Neotropics. After invading Spain in 2006, it spread rapidly throughout Afro-Eurasia and has become a major threat world production. Integrated pest management (IPM) strategies have been developed, but widespread insecticide use caused selection for resistance as well undesirable effects on key beneficial arthropods. Augmentation conservation biological control relying omnivorous mirid predators proved successful of T. absoluta, where implementation dependent abiotic, biotic (e.g., alternative prey), anthropogenic factors pesticides). Research carried out larval parasitoids, showing potential further development sustainable control. resistant varieties ongoing, they are not commercially available yet. Knowledge gaps remain be filled optimize IPM packages crops help prevent worldwide.

Язык: Английский

Процитировано

507
Management of insecticide resistance in the major Aedes vectors of arboviruses: Advances and challenges DOI Creative Commons
Isabelle Dusfour, John Vontas, Jean‐Philippe David

и другие.

PLoS neglected tropical diseases, Год журнала: 2019, Номер 13(10), С. e0007615 - e0007615

Опубликована: Окт. 10, 2019

Background The landscape of mosquito-borne disease risk has changed dramatically in recent decades, due to the emergence and reemergence urban transmission cycles driven by invasive Aedes aegypti Ae. albopictus. Insecticide resistance is already widespread yellow fever mosquito, Aegypti; emerging Asian tiger mosquito Albopictus; now threatening global fight against human arboviral diseases such as dengue, fever, chikungunya, Zika. Because panel insecticides available for public health limited, it primary importance preserve efficacy existing upcoming active ingredients. Timely implementation insecticide management (IRM) crucial maintain arsenal effective sustain arbovirus vector control. Methodology principal findings This Review one a series being generated Worldwide Network (WIN) aims at defining principles concepts underlying IRM, identifying main factors affecting evolution resistance, evaluating value tools monitoring. Based on lessons taken from strategies used other species agricultural pests, we propose framework IRM vectors. Conclusions significance Although should be fixture all control programs, currently often absent strategic plans diseases, especially arboviruses. Experiences vectors pests underscore need urgent action implementing mosquitoes. plan developed malaria vectors, here some key activities establish spp.

Язык: Английский

Процитировано

246
Sublethal exposure, insecticide resistance, and community stress DOI Creative Commons
R. N. C. Guedes, Spencer S. Walse, James E. Throne

и другие.

Current Opinion in Insect Science, Год журнала: 2017, Номер 21, С. 47 - 53

Опубликована: Май 22, 2017

Язык: Английский

Процитировано

230
Insecticide resistance in the tomato pinworm Tuta absoluta: patterns, spread, mechanisms, management and outlook DOI Open Access
R. N. C. Guedes, Emmanouil Roditakis, Mateus Ribeiro de Campos

и другие.

Journal of Pest Science, Год журнала: 2019, Номер 92(4), С. 1329 - 1342

Опубликована: Янв. 31, 2019

Язык: Английский

Процитировано

225
Integrated pest management of Tuta absoluta: practical implementations across different world regions DOI
Nicolas Desneux, Peng Han, Ramzi Mansour

и другие.

Journal of Pest Science, Год журнала: 2021, Номер 95(1), С. 17 - 39

Опубликована: Окт. 31, 2021

Язык: Английский

Процитировано

190
Diamide resistance: 10 years of lessons from lepidopteran pests DOI Creative Commons
Ewan Richardson, Bartlomiej J. Troczka,

Oliver Gutbrod

и другие.

Journal of Pest Science, Год журнала: 2020, Номер 93(3), С. 911 - 928

Опубликована: Март 13, 2020

Abstract Diamide insecticides selectively acting on insect ryanodine receptors (RyR) were launched to the market more than 10 years ago, particularly targeted for control of lepidopteran pest species in diverse agronomic and horticultural cropping systems. They are now globally registered many countries provide reliable levels most settings. However, their frequent application, due alternative mode action chemistries often not providing sufficient control, has resulted selection diamide resistance some world’s destructive species, including populations diamondback moth, tomato leafminer, rice stem borer recently beet armyworm. High resistance, compromising efficacy at recommended field rates, been shown be conferred by RyR target-site mutations affecting binding. The present work reviews global status insecticide pests, with special reference alterations. Furthermore, we discuss principles enabling prediction impact spread based population genetics associated fitness costs as influenced known described. In this context, reiterate calls Insecticide Resistance Action Committee implement effective management following a three-step strategy identification, tracking according protocols discussed article.

Язык: Английский

Процитировано

151
A four-year survey on insecticide resistance and likelihood of chemical control failure for tomato leaf miner Tuta absoluta in the European/Asian region DOI
Emmanouil Roditakis, Emmanouil Vasakis,

Lidia García‐Vidal

и другие.

Journal of Pest Science, Год журнала: 2017, Номер 91(1), С. 421 - 435

Опубликована: Июль 10, 2017

Язык: Английский

Процитировано

138
Stored grain pest prevalence and insecticide resistance in Egyptian populations of the red flour beetle Tribolium castaneum (Herbst) and the rice weevil Sitophilus oryzae (L.) DOI
Manal A. Attia, Trandil F. Wahba, Nehal Shaarawy

и другие.

Journal of Stored Products Research, Год журнала: 2020, Номер 87, С. 101611 - 101611

Опубликована: Апрель 7, 2020

Язык: Английский

Процитировано

115
Monitoring of Target-Site Mutations Conferring Insecticide Resistance in Spodoptera frugiperda DOI Creative Commons
Debora Boaventura,

Macarena Martin,

Alberto Pozzebon

и другие.

Insects, Год журнала: 2020, Номер 11(8), С. 545 - 545

Опубликована: Авг. 18, 2020

Fall armyworm (FAW), Spodoptera frugiperda, a major pest of corn and native to the Americas, recently invaded (sub)tropical regions worldwide. The intensive use insecticides high adoption crops expressing Bacillus thuringiensis (Bt) proteins has led many cases resistance. Target-site mutations are among main mechanisms resistance monitoring their frequency is great value for insecticide management. Pyrosequencing PCR-based allelic discrimination assays were developed used genotype target-site alleles in 34 FAW populations from different continents. diagnostic methods revealed acetylcholinesterase, conferring organophosphates carbamates. In voltage-gated sodium channels targeted by pyrethroids, only one population Indonesia showed mutation. No detected ryanodine receptor, suggesting susceptibility diamides. Indels ATP-binding cassette transporter C2 associated with Bt-resistance observed samples collected Puerto Rico Brazil. Additionally, we analyzed all presence markers two sympatric host plant strains. molecular established show robust results across broad geographical range can be support decisions sustainable control applied

Язык: Английский

Процитировано

80
Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana DOI Creative Commons
Giovanni Benelli,

Lucia Pavoni,

Valeria Zeni

и другие.

Nanomaterials, Год журнала: 2020, Номер 10(9), С. 1867 - 1867

Опубликована: Сен. 18, 2020

The growing interest in the development of green pest management strategies is leading to exploitation essential oils (EOs) as promising botanical pesticides. In this respect, nanotechnology could efficiently support use EOs through their encapsulation into stable nanoformulations, such nanoemulsions (NEs), improve stability and efficacy. This technology assures improvement chemical stability, hydrophilicity, environmental persistence EOs, giving an added value for fabrication natural insecticides effective against a wide spectrum insect vectors pests public agronomical importance. Carlina acaulis (Asteraceae) root EO has been recently proposed ingredient new generation insecticides. present study, highly C. acaulis-based NE was developed. Interestingly, nanosystem able encapsulate 6% (w/w) EO, showing mean diameter around 140 nm SOR (surfactant-to-oil ratio) 0.6. Its evaluated storage period six months corroborated by accelerated study. Therefore, were toxicity 1st instar larvae European grapevine moth (EGVM), Lobesia botrana (Denis & Schiffermüller, 1775) (Lepidoptera: Tortricidae), major vineyard pest. composition investigated gas chromatography–mass spectrometry (GC–MS) revealing carlina oxide, polyacetylene, main constituent. assays, both toxic L. larvae, with LC50 values 7.299 9.044 µL/mL NE, respectively. represents option develop management. To date, study first evidence about insecticidal EO-based NEs

Язык: Английский

Процитировано

72