Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 24, 2024
Double-stranded
RNA
(dsRNA)
has
emerged
as
key
player
in
gene
silencing
for
the
past
two
decades.
Tailor-made
dsRNA
is
now
recognized
a
versatile
raw
material,
suitable
wide
range
of
applications
biopesticide
formulations,
including
insect
control
to
pesticide
resistance
management.
The
mechanism
interference
(RNAi)
acts
at
messenger
(mRNA)
level,
utilizing
sequence-dependent
approach
that
makes
it
unique
term
effectiveness
and
specificity
compared
conventional
agrochemicals.
Two
primary
categories
small
RNAs,
known
short
interfering
RNAs
(siRNAs)
microRNAs
(miRNAs),
function
both
somatic
germline
lineages
broad
eukaryotic
species
regulate
endogenous
genes
defend
genome
from
invasive
nucleic
acids.
Furthermore,
application
RNAi
crop
protection
can
be
achieved
by
employing
plant-incorporated
protectants
through
plant
transformation,
but
also
non-transformative
strategies
such
use
formulations
sprayable
direct
agents,
factor
repressors
or
developmental
disruptors.
This
review
explores
agricultural
RNAi,
delving
into
its
successes
pest-insect
considering
broader
potential
managing
pathogens,
nematodes,
pests.
Additionally,
tool
addressing
pesticide-resistant
weeds
insects
reviewed,
along
with
an
evaluation
production
costs
environmental
implications.
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 28, 2025
Camphora
longepaniculata
is
an
important
economic
crop
renowned
for
its
rich
volatile
terpene
compounds.
Terpene
synthases
(TPS)
are
key
enzymes
in
the
biosynthesis
of
these
compounds,
playing
significant
roles
plant
growth,
development,
and
secondary
metabolism.
In
this
study,
a
total
86
TPS
genes
were
identified
,
which
classified
into
five
groups
based
on
their
evolutionary
relationships.
Analysis
cis-regulatory
elements
revealed
associations
between
processes
related
to
environmental
stress
responses.
Gene
Ontology
(GO)
enrichment
analysis
indicated
that
predominantly
linked
various
enzymatic
activities.
Furthermore,
duplication
events
tandem
duplications
(TD)
whole
genome
(WGD)
major
driving
forces
evolution
gene
family.
Notably,
18
found
be
upregulated
high
essential
oil
content
varieties
.
RT-qPCR
validation
further
confirmed
TPS26
TPS28
TPS47
exhibit
expression
during
leaf
highlighting
potential
involvement
crucial
developmental
stage.
These
findings
lay
solid
foundation
exploration
functions
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 20, 2025
Expression
of
double-stranded
RNAs
(dsRNAs)
in
plants
is
an
emerging
strategy
to
efficiently
control
insects.
RNA
interference
(RNAi)-mediated
pest
takes
advantage
that
can
suppress
the
expression
one
or
more
insect
genes
encode
key
proteins.
Virus-induced
gene
silencing
(VIGS)
a
useful
tool
for
plant
dsRNAs
pests
without
altering
plant's
genome.
Trehalase
(TRE)
and
chitin
synthase
(CHS)
are
very
important
In
this
study,
we
first
demonstrated
spraying
targeting
CHS
TRE
increased
mortality
rate
peach
aphid
Myzus
persicae
treated
with
pathogenic
fungus
Metarhizium
anisopliae.
When
mpTRE
mpCHS
were
expressed
via
VIGS,
was
reduced
aphids,
their
fertility
survival
rates
decreased.
These
results
indicate
VIGS-mediated
powerful
approach
effectively
aphids
had
higher
when
M.
anisopliae
sprayed.
Abstract
Trehalose-6-phosphate
(T6P),
an
intermediate
in
trehalose
metabolic
pathways,
is
ubiquitously
present
nearly
all
cellular
organisms
except
vertebrates.
The
most
well-characterized
route
involves
its
synthesis
by
trehalose-6-phosphate
synthase
(TPS)
and
dephosphorylation
to
phosphatase
(TPP)
the
TPS/TPP
pathway.
Besides,
alternative
pathways
aslo
exist.
In
addition
being
precursor
of
synthesis,
T6P
functions
as
a
signal
molecule
regulating
various
biological
processes.
plants,
inhibits
SnRK1
(Sucrose-nonfermenting
1
Related
Kinase
1),
while
fungi,
primarily
hexokinase
regulates
glycolysis.
Notably,
TPS
TPP
themselves
also
have
some
regulatory
functions.
Genetic
studies
reveal
that
deletion
or
usually
causes
developmental
virulence
defects
bacteria
invertebrates.
Given
important
pathogenic
fungi
but
are
absent
humans
vertebrates,
they
ideal
targets
for
fungicide
development.
This
review
summarizes
multifaceted
roles
invertebrates,
providing
comprehensive
overview
Additionally,
it
discusses
reported
inhibitor
offer
insights
pathogen
control
strategies.
Insects,
Год журнала:
2023,
Номер
14(12), С. 903 - 903
Опубликована: Ноя. 23, 2023
Rapid
cold
hardening
(RCH)
is
known
to
rapidly
enhance
the
tolerance
of
insects.
Trehalose
has
been
demonstrated
be
a
cryoprotectant
in
Lissorhoptrus
oryzophilus,
an
important
invasive
pest
rice
China.
synthesis
mainly
occurs
through
Trehalose-6-phosphate
synthase
(TPS)/trehalose-6-phosphate
phosphatase
(TPP)
pathway
In
this
study,
TPS
gene
from
L.
oryzophilus
(LoTPS)
was
cloned
and
characterized
for
first
time.
Its
expression
trehalose
content
changes
elicited
by
RCH
were
investigated.
Our
results
revealed
that
not
only
increased
survival
rate
adults
but
also
upregulated
level
LoTPS
under
low
temperature.
We
hypothesized
promoted
accumulation
protect
low-temperature
damage.
To
further
verify
function
gene,
we
employed
RNA
interference
(RNAi)
technology.
findings
showed
efficiency
disappeared
did
increase
when
fed
dsRNA
LoTPS.
Additionally,
inhibiting
resulted
no
significant
difference
between
non-RCH
treatments.
Furthermore,
patterns
transporter
(TRET)
trehalase
(TRE)
affected.
Collectively,
these
indicate
critical
role
resistance
after
induction.
can
ability
regulating
metabolism.
These
contribute
understanding
insect
invasiveness
oryzophilus.
Moreover,
RNAi
opens
up
possibilities
novel
control
strategies
against
future.
RCH
is
known
rapidly
enhance
cold
tolerance
of
insects.
Trehalose
has
been
demonstrated
to
be
one
the
cryoprotectant
in
L.
oryzophilus,
an
important
invasive
pest
rice
China.
synthesis
mainly
occurs
through
TPS/TPP
pathway
In
this
study,
LoTPS
gene
was
cloned
and
characterized
for
first
time.
Its
expression
trehalose
content
changes
elicited
by
were
investigated.
Our
results
revealed
that
not
only
increased
survival
rate
adults
but
also
upregulated
level
under
low
temperature.
We
hypothesized
promoted
accumulation
protect
from
low-temperature
damage.
To
further
verify
function
gene,
we
employed
RNAi
technology.
findings
showed
efficiency
disappeared
failed
increase
when
fed
dsRNA
LoTPS.
Additionally,
inhibiting
resulted
no
significant
difference
between
non-RCH
treatments.
Furthermore,
patterns
TRET
TRE
affected.
Collectively,
these
indicate
critical
role
oryzophilus
resistance
after
induction.
can
ability
regulating
metabolism.
These
contribute
understanding
TPS
insect
invasiveness
oryzophilus.
Moreover,
opens
up
possibilities
novel
control
strategies
against
future.
Journal of Applied Entomology,
Год журнала:
2023,
Номер
147(8), С. 611 - 621
Опубликована: Июнь 7, 2023
Abstract
RNA
interference
(RNAi)
is
a
technique
employed
to
investigate
the
functions
of
various
genes
in
whitefly
and
other
insects.
The
effectiveness
RNAi
depends
upon
delivery
dsRNA
into
insects
it
has
been
achieved
through
microinjection,
oral
feeding,
egg
soaking,
transfection,
topical
application,
spraying
with
nanoparticles,
leaf‐mediated
feeding
or
plant‐mediated/
transgenic
plants.
In
Bemisia
tabaci
Gennadius
(Hemiptera:
Aleyrodidae),
most
studies
on
were
conducted
adult
either
injection
feeding.
There
need
develop
system
deliver
nymphs
because
first
instar
after
hatching
find
suitable
place
leaf
feed
same
till
emergence.
So,
we
evaluated
nine
different
techniques
using
sponges,
tubes,
and,
Petri
plates
for
nymphs.
detached
survived
maximum
number
days
(8.25
days)
minimum
nymphal
mortality
(12.01%)
Whitefly
Nymphal
Feeding
System‐1
(WNFS‐1)
treatment;
therefore,
WNFS‐1
was
selected
B
.
WNFS‐1,
blue
food
dye
used
confirm
that
being
fed,
results
evidenced
by
blue‐coloured
bodies.
juvenile
hormone
esterase
(
jhe
)
gene
silenced
order
successfully
resulting
much
higher
(68.36%).
Therefore,
reliable
method
delivering
purpose
silencing
stages
whiteflies.
WNFS
may
also
be
extensively
evaluate
any
chemistry
(insecticides,
hormones,
biopesticides,
etc.)
whitefly.
