Role of mitochondrial complex I genes in host plant expansion of Bactrocera tau (Tephritidae: Diptera) by CRISPR/Cas9 system
Insect Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 19, 2025
Abstract
Host
expansion
facilitates
tephritid
flies
to
expand
their
ranges.
Unraveling
the
mechanisms
of
host
will
help
efficiently
control
these
pests.
Our
previous
works
showed
mitochondrial
complex
I
genes
Ndufs1
,
Ndufs3
and
Ndufa7
being
upregulated
during
Bactrocera
tau
(Walker),
one
highly
hazardous
species
tephritids.
However,
roles
in
B.
remain
unknown.
Here,
using
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
/
CRISPR‐associated
nuclease
9
(Cas9)
editing
system
for
first
time,
a
stable
homozygous
strain
(
Btndufa7
−/−
),
heterozygous
Btndufs1
+/−
strains
Btndufs3
)
were
obtained
from
F3
generation
after
gene
knockout.
Reduced
sizes
larvae
pupae
knockout
observed.
Notably,
mean
values
fitness
estimation
(pupal
numbers,
single‐pupal
weight
emergence
rate)
expression
slightly
reduced
on
2
native
hosts
(summer
squash
cucumber),
while
it
sharply
decreased
novel
banana
potential
pitaya,
compared
with
those
wild‐type
strain.
Furthermore,
did
not
survive
guava.
These
results
suggested
that
disturbs
survival
hosts,
further
.
Homozygous
lethality
occurred
or
suggesting
play
role
early
development
This
study
revealed
is
target
management
tephritids
opens
new
avenue
pest
research.
Язык: Английский
Alternative fumigation technologies for Bursaphelenchus xylophilus‐infected woods: reducing reliance on methyl bromide
Hao Yang,
Yuqi Hua,
Tao Liu
и другие.
Pest Management Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 25, 2025
Abstract
BACKGROUND
Pine
wilt
disease
(PWD),
caused
by
the
pine
wood
nematode
(PWN)
Bursaphelenchus
xylophilus
(Steiner
&
Buhrer)
Nickle,
poses
a
significant
global
threat
to
forests.
This
study
investigated
nematicidal
efficacy
of
four
fumigants—methyl
bromide
(MB),
phosphine
(PH
3
),
sulfuryl
fluoride
(SF),
and
methyl
isothiocyanate
(MITC)—applied
both
individually
in
combination
+MB,
SF+MB,
MITC+MB,
PH
+SF,
+MITC,
MITC+SF)
against
PWN.
RESULTS
Under
uniform
fumigation
conditions,
,
SF,
MITC
demonstrated
effects
comparable
with
MB.
Furthermore,
these
fumigants
MB
exhibited
synergistic
eradicating
Compared
treatments
individual
fumigants,
incorporating
small
amounts
or
reduced
required
dosage
at
least
60%
achieve
same
mortality
rate.
Optimal
efficiency
was
observed
ratios
1:9
for
:MB,
SF:MB,
3:7
MITC:MB.
Although
SF
showed
an
insignificant
effect,
either
pronounced
synergy,
optimal
being
:MITC
5:5
SF:MITC.
CONCLUSION
highlights
as
potential
alternatives
controlling
Moreover,
strong
interactions
when
combining
offer
promising
strategy
reduce
dependency.
notable
synergy
between
further
supports
their
utility
integrated
approaches
PWN
management.
©
2025
Society
Chemical
Industry.
Язык: Английский
Toxicity of Ethyl Formate to Tribolium castaneum (Herbst) Exhibiting Different Levels of Phosphine Resistance and Its Influence on Metabolite Profiles
Agriculture,
Год журнала:
2024,
Номер
14(2), С. 323 - 323
Опубликована: Фев. 18, 2024
Ethyl
formate
(EF),
a
naturally
occurring
fumigant,
has
attracted
widespread
attention
owing
to
its
low
toxicity
in
mammals.
Here,
Direct
Immersion
Solid-Phase
Microextraction
(DI-SPME)
was
employed
for
sample
preparation
mass
spectrometry-based
untargeted
metabolomics
evaluate
the
effects
on
Tribolium
castaneum
(Herbst)
strains
with
different
levels
of
PH3
resistance
(sensitive,
TC-S;
moderately
resistant,
TC-M;
strongly
TC-SR)
when
exposed
sub-lethal
concentration
(LC30)
EF.
The
bioassay
indicated
that
T.
varying
did
not
confer
cross-resistance
A
metabolomic
analysis
revealed
exposure
sublethal
doses
EF
significantly
altered
23
metabolites
castaneum,
including
2
are
unique
species
which
remained
unaffected
by
external
conditions,
while
11
compounds
showed
strong
response.
pathway
topology
caused
changes
several
metabolic
pathways,
mainly
involving
fatty
acids
and
their
related
pathways.
This
study
can
induce
highly
similar
responses
insects
across
resistance,
suggesting
mechanisms
driving
distinct.
These
insights
extend
our
knowledge
toxic
provide
direct
evidence
efficacy
treatment
managing
insects.
Язык: Английский
Development and Metabolomic Profiles of Bactrocera dorsalis (Diptera: Tephritidae) Larvae Exposed to Phytosanitary Irradiation Dose in Hypoxic Environment Using DI-SPME-GC/MS
Insects,
Год журнала:
2024,
Номер
15(3), С. 177 - 177
Опубликована: Март 6, 2024
X-ray
irradiation
and
modified
atmospheres
(MAs)
provide
eco-friendly,
chemical-free
methods
for
pest
management.
Although
a
low-oxygen
atmospheric
treatment
improves
the
performance
of
some
irradiated
insects,
its
influence
on
quarantine
insects
impacts
control
efficacy
have
yet
to
be
investigated.
Based
bioassay
results,
this
study
employed
direct
immersion
solid-phase
microextraction
(DI-SPME)
combined
with
gas
chromatography-mass
spectrometry
(GC-MS)
determine
metabolic
profiles
late
third-instar
B.
dorsalis
larvae
under
normoxia
(CON,
Air),
hypoxia
(95%
N2
+
5%
O2,
HY),
super-hypoxia
(99.5%
0.5%
Sup-HY),
irradiation-alone
(116
Gy,
IR-alone),
(HY
IR)
(Sup-HY
IR).
Our
findings
reveal
that,
compared
IR-alone
group,
IR
HY
Sup-HY
increases
larval
pupation
dorsalis,
weakens
delaying
effect
developmental
stage.
However,
these
3
groups
further
hinder
adult
emergence
phytosanitary
dose
116
Gy.
Moreover,
all
IR-treated
groups,
including
IR-alone,
IR,
lead
insect
death
as
coarctate
or
pupae.
Pathway
analysis
identified
changed
pathways
across
groups.
Specifically,
changes
in
lipid
metabolism-related
were
observed:
vs.
CON,
2
5
each
CON.
The
treatments
induce
comparable
modifications
pathways.
demonstrate
significantly
fewer
changes.
research
suggests
that
environment
Sup-HY)
might
enhance
radiation
tolerance
by
stabilizing
metabolism
at
biologically
feasible
levels.
Additionally,
our
indicate
current
contributes
effective
management
without
being
influenced
radioprotective
effects.
These
results
hold
significant
importance
understanding
biological
effects
developing
IR-specific
regulatory
guidelines
MA
environments.
Язык: Английский
Sorption and residue analysis of phosphine in fruits and vegetables
New Zealand Journal of Crop and Horticultural Science,
Год журнала:
2024,
Номер
unknown, С. 1 - 11
Опубликована: Сен. 30, 2024
Язык: Английский
Phosphine and phosphine plus ethyl formate for controlling papaya mealybug (Hemiptera: Pseudococcidae) on succulents
Hang Zou,
Li Li,
Baishu Li
и другие.
Journal of Economic Entomology,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 2, 2024
Abstract
The
papaya
mealybug,
Paracoccus
marginatus
(Hemiptera:
Pseudococcidae),
poses
a
serious
threat
to
global
trade
and
is
classified
as
quarantine
pest
in
some
countries.
Phosphine
(PH3)
considered
an
alternative
methyl
bromide
for
postharvest
control
of
P.
marginatus.
However,
reports
addressing
the
impact
PH3
treatment
on
succulent
plants
are
lacking.
This
study
aims
investigate
effects
fumigation
alone
combination
with
ethyl
formate
(EF)
insecticidal
activity
across
all
developmental
stages
its
influence
quality
plant
products.
Tolerance
tests
showed
that
among
stages,
egg
stage
most
tolerant
treatment.
A
concentration
1
g
m−3
8
h
exposure
at
25
°C
achieved
99.9968%
(Probit-9)
mortality
rate
eggs.
Additionally,
we
examined
synergistic
combining
EF
PH3.
Combined
0.5
2
did
not
adversely
affect
lifespan
three
species,
achieving
effective
dose
0.99991.
These
findings
suggest
fumigation,
or
without
EF,
treatments
plants.
Язык: Английский