Phosphine and phosphine plus ethyl formate for controlling papaya mealybug (Hemiptera: Pseudococcidae) on succulents DOI

Hang Zou,

Li Li,

Baishu Li

et al.

Journal of Economic Entomology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 2, 2024

Abstract The papaya mealybug, Paracoccus marginatus (Hemiptera: Pseudococcidae), poses a serious threat to global trade and is classified as quarantine pest in some countries. Phosphine (PH3) considered an alternative methyl bromide for postharvest control of P. marginatus. However, reports addressing the impact PH3 treatment on succulent plants are lacking. This study aims investigate effects fumigation alone combination with ethyl formate (EF) insecticidal activity across all developmental stages its influence quality plant products. Tolerance tests showed that among stages, egg stage most tolerant treatment. A concentration 1 g m−3 8 h exposure at 25 °C achieved 99.9968% (Probit-9) mortality rate eggs. Additionally, we examined synergistic combining EF PH3. Combined 0.5 2 did not adversely affect lifespan three species, achieving effective dose 0.99991. These findings suggest fumigation, or without EF, treatments plants.

Language: Английский

Role of mitochondrial complex I genes in host plant expansion of Bactrocera tau (Tephritidae: Diptera) by CRISPR/Cas9 system DOI Creative Commons
Wei Shi, Linsheng He, Ruixiang Li

et al.

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 19, 2025

Abstract Host expansion facilitates tephritid flies to expand their ranges. Unraveling the mechanisms of host will help efficiently control these pests. Our previous works showed mitochondrial complex I genes Ndufs1 , Ndufs3 and Ndufa7 being upregulated during Bactrocera tau (Walker), one highly hazardous species tephritids. However, roles in B. remain unknown. Here, using clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR‐associated nuclease 9 (Cas9) editing system for first time, a stable homozygous strain ( Btndufa7 −/− ), heterozygous Btndufs1 +/− strains Btndufs3 ) were obtained from F3 generation after gene knockout. Reduced sizes larvae pupae knockout observed. Notably, mean values fitness estimation (pupal numbers, single‐pupal weight emergence rate) expression slightly reduced on 2 native hosts (summer squash cucumber), while it sharply decreased novel banana potential pitaya, compared with those wild‐type strain. Furthermore, did not survive guava. These results suggested that disturbs survival hosts, further . Homozygous lethality occurred or suggesting play role early development This study revealed is target management tephritids opens new avenue pest research.

Language: Английский

Citations

0
Toxicity of Ethyl Formate to Tribolium castaneum (Herbst) Exhibiting Different Levels of Phosphine Resistance and Its Influence on Metabolite Profiles DOI Creative Commons
Changyao Shan, X. Z. You, Li Li

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 14(2), P. 323 - 323

Published: Feb. 18, 2024

Ethyl formate (EF), a naturally occurring fumigant, has attracted widespread attention owing to its low toxicity in mammals. Here, Direct Immersion Solid-Phase Microextraction (DI-SPME) was employed for sample preparation mass spectrometry-based untargeted metabolomics evaluate the effects on Tribolium castaneum (Herbst) strains with different levels of PH3 resistance (sensitive, TC-S; moderately resistant, TC-M; strongly TC-SR) when exposed sub-lethal concentration (LC30) EF. The bioassay indicated that T. varying did not confer cross-resistance A metabolomic analysis revealed exposure sublethal doses EF significantly altered 23 metabolites castaneum, including 2 are unique species which remained unaffected by external conditions, while 11 compounds showed strong response. pathway topology caused changes several metabolic pathways, mainly involving fatty acids and their related pathways. This study can induce highly similar responses insects across resistance, suggesting mechanisms driving distinct. These insights extend our knowledge toxic provide direct evidence efficacy treatment managing insects.

Language: Английский

Citations

3
Development and Metabolomic Profiles of Bactrocera dorsalis (Diptera: Tephritidae) Larvae Exposed to Phytosanitary Irradiation Dose in Hypoxic Environment Using DI-SPME-GC/MS DOI Creative Commons
Changyao Shan,

Baishu Li,

Li Li

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(3), P. 177 - 177

Published: March 6, 2024

X-ray irradiation and modified atmospheres (MAs) provide eco-friendly, chemical-free methods for pest management. Although a low-oxygen atmospheric treatment improves the performance of some irradiated insects, its influence on quarantine insects impacts control efficacy have yet to be investigated. Based bioassay results, this study employed direct immersion solid-phase microextraction (DI-SPME) combined with gas chromatography-mass spectrometry (GC-MS) determine metabolic profiles late third-instar B. dorsalis larvae under normoxia (CON, Air), hypoxia (95% N2 + 5% O2, HY), super-hypoxia (99.5% 0.5% Sup-HY), irradiation-alone (116 Gy, IR-alone), (HY IR) (Sup-HY IR). Our findings reveal that, compared IR-alone group, IR HY Sup-HY increases larval pupation dorsalis, weakens delaying effect developmental stage. However, these 3 groups further hinder adult emergence phytosanitary dose 116 Gy. Moreover, all IR-treated groups, including IR-alone, IR, lead insect death as coarctate or pupae. Pathway analysis identified changed pathways across groups. Specifically, changes in lipid metabolism-related were observed: vs. CON, 2 5 each CON. The treatments induce comparable modifications pathways. demonstrate significantly fewer changes. research suggests that environment Sup-HY) might enhance radiation tolerance by stabilizing metabolism at biologically feasible levels. Additionally, our indicate current contributes effective management without being influenced radioprotective effects. These results hold significant importance understanding biological effects developing IR-specific regulatory guidelines MA environments.

Language: Английский

Citations

0
Sorption and residue analysis of phosphine in fruits and vegetables DOI
Anisha Kathpalia, Sumitra Arora, Jai Gopal Sharma

et al.

New Zealand Journal of Crop and Horticultural Science, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 11

Published: Sept. 30, 2024

Language: Английский

Citations

0
Phosphine and phosphine plus ethyl formate for controlling papaya mealybug (Hemiptera: Pseudococcidae) on succulents DOI

Hang Zou,

Li Li,

Baishu Li

et al.

Journal of Economic Entomology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 2, 2024

Abstract The papaya mealybug, Paracoccus marginatus (Hemiptera: Pseudococcidae), poses a serious threat to global trade and is classified as quarantine pest in some countries. Phosphine (PH3) considered an alternative methyl bromide for postharvest control of P. marginatus. However, reports addressing the impact PH3 treatment on succulent plants are lacking. This study aims investigate effects fumigation alone combination with ethyl formate (EF) insecticidal activity across all developmental stages its influence quality plant products. Tolerance tests showed that among stages, egg stage most tolerant treatment. A concentration 1 g m−3 8 h exposure at 25 °C achieved 99.9968% (Probit-9) mortality rate eggs. Additionally, we examined synergistic combining EF PH3. Combined 0.5 2 did not adversely affect lifespan three species, achieving effective dose 0.99991. These findings suggest fumigation, or without EF, treatments plants.

Language: Английский

Citations

0