Immunomodulating melatonin-decorated silica nanoparticles suppress bacterial wilt (Ralstonia solanacearum) in tomato (Solanum lycopersicum L.) through fine-tuning of oxidative signaling and rhizosphere bacterial community
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Окт. 12, 2024
Tomato
(Solanum
lycopersicum
L.)
production
is
severely
threatened
by
bacterial
wilt,
caused
the
phytopathogenic
bacterium
Ralstonia
solanacearum.
Recently,
nano-enabled
strategies
have
shown
tremendous
potential
in
crop
disease
management.
This
study
investigates
efficacy
of
biogenic
nanoformulations
(BNFs),
comprising
silica
nanoparticles
(SiNPs)
and
melatonin
(MT),
controlling
wilt
tomato.
SiNPs
were
synthesized
using
Zizania
latifolia
leaves
extract.
Further,
MT
containing
BNFs
through
one-pot
approach.
Nanomaterials
characterized
standard
characterization
techniques.
Greenhouse
assays
conducted
to
assess
impact
on
tomato
plant
immunity
resistance
wilt.
The
exhibited
a
spherical
morphology,
with
particle
sizes
ranging
from
13.02
nm
22.33
for
17.63
21.79
BNFs,
indicating
relatively
uniform
size
distribution
consistent
shape
across
both
materials.
experiments
revealed
that
soil
application
outperformed
SiNPs,
significantly
enhancing
reducing
incidence
78.29%
plants
maintaining
oxidative
stress
homeostasis
via
increasing
activities
antioxidant
enzymes
such
as
superoxide
dismutase
(31.81%),
peroxidase
(32.9%),
catalase
(32.65%),
ascorbate
(47.37%)
compared
untreated
infected
plants.
Additionally,
induced
salicylic
acid
activating
defense-related
genes
(e.g.,
SlPAL1,
SlICS1,
SlNPR1,
SlEDS,
SlPD4,
SlSARD1)
involved
phytohormones
signaling
High-throughput
16
S
rRNA
sequencing
promoted
growth
beneficial
rhizosphere
bacteria
(Gemmatimonadaceae,
Ramlibacter,
Microscillaceae,
Anaerolineaceae,
Chloroplast
Phormidium)
healthy
diseased
plants,
while
suppressing
R.
solanacearum
abundance
Overall,
these
findings
suggest
offer
more
promising
sustainable
approach
managing
Язык: Английский
Resistance to the SDHI Fungicide Pydiflumetofen in Fusarium solani: Risk Assessment and Resistance-Related Point Mutation in FsSdhC Gene
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(44), С. 24325 - 24335
Опубликована: Окт. 28, 2024
Peanut
root
rot
(PRR)
is
a
prevalent
and
destructive
plant
disease
attributed
to
Язык: Английский
Genomic Insights into the Bactericidal and Fungicidal Potential of Bacillus mycoides b12.3 Isolated in the Soil of Olkhon Island in Lake Baikal, Russia
Microorganisms,
Год журнала:
2024,
Номер
12(12), С. 2450 - 2450
Опубликована: Ноя. 28, 2024
The
dispersal
of
plant
pathogens
is
a
threat
to
the
global
economy
and
food
industry
which
necessitates
need
discover
efficient
biocontrol
agents
such
as
bacteria,
fungi,
etc.,
inhibiting
them.
Here,
we
describe
Bacillus
mycoides
strain
b12.3
isolated
from
soil
Olkhon
Island
in
Lake
Baikal,
Russia.
By
applying
co-cultivation
technique,
found
that
inhibits
growth
pathogens,
bacteria
Xanthomonas
campestris,
Clavibacter
michiganensis,
Pectobacterium
atrospecticum,
well
fungus
Alternaria
solani.
To
elucidate
genomic
fundament
explaining
these
activities,
leveraged
next-generation
whole-genome
sequencing
obtained
high-quality
assembly
based
on
short
reads.
isolate
bore
seven
known
BGCs
(biosynthetic
gene
clusters),
including
those
responsible
for
producing
bacillibactin,
fengycin,
petrobactin.
Moreover,
genome
contained
insecticidal
genes
encoding
App4Aa1,
Tpp78Ba1,
Spp1Aa1
toxins,
thus
implicating
possible
pesticidal
potential.
We
compared
with
50
closest
assemblies
enriched
BGCs.
analysis
also
revealed
architecture
corresponds
experimentally
observed
activity
spectrum
implying
combination
produced
secondary
metabolites
delineates
range
inhibited
phytopathogens
Therefore,
this
study
deepens
our
knowledge
biology
ecology
B.
residing
Baikal
region.
Язык: Английский