Immunomodulating melatonin-decorated silica nanoparticles suppress bacterial wilt (Ralstonia solanacearum) in tomato (Solanum lycopersicum L.) through fine-tuning of oxidative signaling and rhizosphere bacterial community

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 12, 2024

Tomato (Solanum lycopersicum L.) production is severely threatened by bacterial wilt, caused the phytopathogenic bacterium Ralstonia solanacearum. Recently, nano-enabled strategies have shown tremendous potential in crop disease management. This study investigates efficacy of biogenic nanoformulations (BNFs), comprising silica nanoparticles (SiNPs) and melatonin (MT), controlling wilt tomato. SiNPs were synthesized using Zizania latifolia leaves extract. Further, MT containing BNFs through one-pot approach. Nanomaterials characterized standard characterization techniques. Greenhouse assays conducted to assess impact on tomato plant immunity resistance wilt. The exhibited a spherical morphology, with particle sizes ranging from 13.02 nm 22.33 for 17.63 21.79 BNFs, indicating relatively uniform size distribution consistent shape across both materials. experiments revealed that soil application outperformed SiNPs, significantly enhancing reducing incidence 78.29% plants maintaining oxidative stress homeostasis via increasing activities antioxidant enzymes such as superoxide dismutase (31.81%), peroxidase (32.9%), catalase (32.65%), ascorbate (47.37%) compared untreated infected plants. Additionally, induced salicylic acid activating defense-related genes (e.g., SlPAL1, SlICS1, SlNPR1, SlEDS, SlPD4, SlSARD1) involved phytohormones signaling High-throughput 16 S rRNA sequencing promoted growth beneficial rhizosphere bacteria (Gemmatimonadaceae, Ramlibacter, Microscillaceae, Anaerolineaceae, Chloroplast Phormidium) healthy diseased plants, while suppressing R. solanacearum abundance Overall, these findings suggest offer more promising sustainable approach managing

Language: Английский

Genomic Insights into the Bactericidal and Fungicidal Potential of Bacillus mycoides b12.3 Isolated in the Soil of Olkhon Island in Lake Baikal, Russia

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2450 - 2450

Published: Nov. 28, 2024

The dispersal of plant pathogens is a threat to the global economy and food industry which necessitates need discover efficient biocontrol agents such as bacteria, fungi, etc., inhibiting them. Here, we describe Bacillus mycoides strain b12.3 isolated from soil Olkhon Island in Lake Baikal, Russia. By applying co-cultivation technique, found that inhibits growth pathogens, bacteria Xanthomonas campestris, Clavibacter michiganensis, Pectobacterium atrospecticum, well fungus Alternaria solani. To elucidate genomic fundament explaining these activities, leveraged next-generation whole-genome sequencing obtained high-quality assembly based on short reads. isolate bore seven known BGCs (biosynthetic gene clusters), including those responsible for producing bacillibactin, fengycin, petrobactin. Moreover, genome contained insecticidal genes encoding App4Aa1, Tpp78Ba1, Spp1Aa1 toxins, thus implicating possible pesticidal potential. We compared with 50 closest assemblies enriched BGCs. analysis also revealed architecture corresponds experimentally observed activity spectrum implying combination produced secondary metabolites delineates range inhibited phytopathogens Therefore, this study deepens our knowledge biology ecology B. residing Baikal region.

Language: Английский

Resistance to the SDHI Fungicide Pydiflumetofen in Fusarium solani: Risk Assessment and Resistance-Related Point Mutation in FsSdhC Gene

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(44), P. 24325 - 24335

Published: Oct. 28, 2024

Peanut root rot (PRR) is a prevalent and destructive plant disease attributed to

Language: Английский