A Study on Chemical Characterization and Biological Abilities of Alstonia boonei Extracts Obtained by Different Techniques

Antioxidants, Год журнала: 2022, Номер 11(11), С. 2171 - 2171

Опубликована: Ноя. 1, 2022

In the quest for novel therapeutic agents from plants, choice of extraction solvent and technique plays a key role. this study, possible differences in phytochemical profile bioactivity (antioxidant enzyme inhibitory activity) Alstonia boonei leaves stem bark extracted using water, ethyl acetate methanol, different techniques, namely infusion, maceration Soxhlet extraction, were investigated. Data collected showed that methanol extracts both A. (48.34-53.08 mg gallic acid equivalent [GAE]/g dry extract) (37.08-45.72 GAE/g possessed higher phenolic content compared to (leaves: 30.64-40.19 GAE/g; bark: 34.25-35.64 GAE/g). The radical scavenging reducing capacity, these findings accordance with results. general, water obtained by infusion poor inhibitors acetylcholinesterase, α-amylase, α-glucosidase, tyrosinase, except butyrylcholinesterase. chemical profiles determined UHPLC-MS presence several compounds, such as acids (caffeic, chlorogenic ferulic acids, etc.), flavonoids (rutin isoquercetin) flavonolignans (Cinchonain isomers). Cell viability was tested human peripheral blood monocytic cell line (THP-1), safe up 25 μg/mL. addition, anti-inflammatory effects investigated releasing IL-6 TNF-α IL-1β. particular, exhibited significant effects. presented study highlight role bioactive secondary metabolites plants. appraises antioxidant action bark, which are extensively used traditional medicine.

Язык: Английский

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Therapeutic effects on H1N1-induced pneumonia in mice and intestinal bacteria biotransformation of four main flavonoids from Houttuynia cordata Thunb.

Journal of Pharmaceutical and Biomedical Analysis, Год журнала: 2023, Номер 233, С. 115469 - 115469

Опубликована: Май 18, 2023

Язык: Английский

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New UHPLC-Q-Orbitrap MS/MS-based Library-comparison method simultaneously distinguishes 22 phytophenol isomers from Desmodium styracifolium

Microchemical Journal, Год журнала: 2023, Номер 191, С. 108938 - 108938

Опубликована: Июнь 3, 2023

Язык: Английский

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Comparative Assessment of the Antioxidant and Anticancer Activities of Plicosepalus acacia and Plicosepalus curviflorus: Metabolomic Profiling and In Silico Studies

Antioxidants, Год журнала: 2022, Номер 11(7), С. 1249 - 1249

Опубликована: Июнь 25, 2022

This study presents a comparison between two mistletoe plants-P. acacia and P. curviflorus-regarding their total phenolic contents antioxidant anticancer activities. curviflorus exhibited higher phenolics content (340.62 ± 19.46 mg GAE/g extract), demonstrated DPPH free radical scavenging activity (IC50 = 48.28 3.41µg/mL), stronger reducing power (1.43 0.54 mMol Fe+2/g) for ferric ions, greater capacity (41.89 3.15 GAE/g) compared to acacia. The cytotoxic effects of methanol extracts were examined on lung (A549), prostate (PC-3), ovarian (A2780) breast (MDA-MB-231) cancer cells. highest potential the was observed PC-3 cells, where more pronounced antiproliferative 25.83 μg/mL) than 34.12 μg/mL). In addition, both tested arrested cell cycle at Pre-G1 G1 phases, induced apoptosis. However, extract possessed apoptotic effect, mediated by upregulation p53, Bax, caspase-3, 8 9, downregulation Bcl-2 expression. pursuit link chemical diversity with bioactivities, its metabolomic profiling achieved LC-ESI-TOF-MS/MS technique. permitted tentative identification several phenolics-chiefly flavonoid derivatives, beside some triterpenes sterols-in extract. Furthermore, all metabolites in inspected binding modes towards CDK-2 EGFR proteins using molecular docking studies an attempt understand superiority over regarding effect Docking supported our experimental results; this taken together, could be regarded as prospect development chemotherapeutics cancer.

Язык: Английский

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Kuwanon H Inhibits Melanoma Growth through Cytotoxic Endoplasmic Reticulum Stress and Impaired Autophagy Flux

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(37), С. 13768 - 13782

Опубликована: Сен. 6, 2023

Although great progress has been made recently in targeted and immune-based therapies, additional treatments are needed for most melanoma patients due to acquired chemoresistance, recurrence, or metastasis. Elevated autophagy is required the pathogenesis of attenuate metabolic stress, protecting cancer cells from chemotherapeutics radiation. Thus, intervention with a promising strategy treatment. Here, we examined novel antimelanoma natural compound named kuwanon H (KuH), which significantly inhibited cell growth invitro/vivo. Mechanistically, KuH induced cytotoxic endoplasmic reticulum (ER) viability apoptosis. Meanwhile, KuH-induced ER stress mediated autophagysome formation through ATF4-DDIT3-TRIB3-AKT-MTOR axis. Importantly, impaired flux, contributed anticancer effects KuH. Finally, our results showed that enhanced sensitivity cisplatin, both invitro vivo, by impairing degradation reactive oxygen species damaged mitochondria. Our findings indicate candidate product therapy.

Язык: Английский

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Unlocking the Potential: Quercetin and Its Natural Derivatives as Promising Therapeutics for Sepsis

Biomedicines, Год журнала: 2024, Номер 12(2), С. 444 - 444

Опубликована: Фев. 16, 2024

Sepsis is a syndrome of organ dysfunction caused by an uncontrolled inflammatory response, which can seriously endanger life. Currently, there still shortage specific therapeutic drugs. Quercetin and its natural derivatives have received lot attention recently for their potential in treating sepsis. Here, we provide comprehensive summary the recent research progress on quercetin derivatives, with focus mechanisms antioxidation anti-inflammation. To obtain necessary information, conducted search PubMed, Web Science, EBSCO, Cochrane library databases using keywords sepsis, anti-inflammatory, antioxidant, anti-infection, quercetin, to identify relevant from 6315 articles published last five years. At present, 11 been intensively studied. They primarily exert anti-inflammation effects through PI3K/AKT/NF-κB, Nrf2/ARE, MAPK pathways. The feasibility these compounds experimental models clinical application were also discussed. In conclusion, good treatment

Язык: Английский

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Overexpression of S30 Ribosomal Protein Leads to Transcriptional and Metabolic Changes That Affect Plant Development and Responses to Stress

Biomolecules, Год журнала: 2024, Номер 14(3), С. 319 - 319

Опубликована: Март 7, 2024

ICT1 is an

Язык: Английский

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The Role of Natural Products from Herbal Medicine in TLR4 Signaling for Colorectal Cancer Treatment

Molecules, Год журнала: 2024, Номер 29(12), С. 2727 - 2727

Опубликована: Июнь 7, 2024

The toll-like receptor 4 (TLR4) signaling pathway constitutes an intricate network of protein interactions primarily involved in inflammation and cancer. This triggers intracellular cascades, modulating transcription factors that regulate gene expression related to immunity malignancy. Previous studies showed colon cancer patients with low TLR4 exhibit extended survival times the holds a significant role CRC pathogenesis. In recent years, traditional Chinese medicines (TCMs) have garnered substantial attention as alternative therapeutic modality for CRC, due their multifaceted composition ability target multiple pathways. Emerging evidence indicates specific TCM products, such andrographolide, rosmarinic acid, baicalin, etc., potential impede development through pathway. Here, we review biochemical processes natural products from TCMs affecting sheds light on treatment strategies utilizing inhibitors which contributes advancement research accelerates clinical integration into treatment.

Язык: Английский

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Impact of extraction techniques and process optimization on antioxidant and antibacterial potential of Kalanchoe pinnata leaf extract

International Journal of Food Properties, Год журнала: 2024, Номер 27(1), С. 909 - 926

Опубликована: Июль 7, 2024

Kalanchoe pinnata (Lam.) Pers. is an ethnomedicinal plant known for its antioxidant, antibacterial, and therapeutic potential. The current study was effort to explore the impact of extraction technique on phytochemical, antibacterial profile extracts. K. leaf extracts were prepared using conventional solvent (100% methanol; 50% methanol: water; 100% water), microwave-assisted at 700-W power (3 min; 6 9 min), supercritical fluid (SFE) (3500 psi; 4500 5500 psi). Best results attained from SFE in terms total phenolic content (240 ± 3 mg GAE/g), flavonoid (165.2 0.4 QE/g), 2,2-diphenyl-1-picrylhydrazyl radical scavenging potential (91.9 0.4%), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid assay value (8.04 0.03 mMol TE/g) ferric reducing antioxidant (471.7 0.8 µg/mL). have been found be most effective against Yersinia pseudotuberculosis (22.20 0.95 mm) least againstProteus vulgaris (0 0 mm). High-performance liquid chromatography analysis extract showed that it contains flavanol quercetin (2.0 0.1 mg/g). It evident findings can effectively treat bacterial infections cure metabolic disorders by oxidative stress. Application advance techniques enhances biological activity

Язык: Английский

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Effect of Liposomal Protium heptaphyllum (Alb.) March Extract in the Treatment of Obesity Induced by High-Calorie Diet

Biology, Год журнала: 2024, Номер 13(7), С. 535 - 535

Опубликована: Июль 17, 2024

Obesity, a chronic disease, resulted from excessive consumption of high-calorie foods, leading to an energy imbalance. Protium heptaphyllum (P. heptaphyllum) was used in folk medicine for its analgesic, anti-inflammatory, and healing properties. The association the extract P. with nanotechnology innovative combining high technology active ingredients that are easily accessible Amazon region. This study evaluated effect liposomes containing ethyl acetate fraction crude leaves on obesity. Male Wistar rats treated diet 8 weeks induce obesity received treatment liposome formulation (1 mg/kg/day, via gavage) 14 days. Morphological, metabolic, redox status, immunological, histological parameters were adipose liver tissue animals. groups divided as follows: C: control; P: extract; O: obese, OP: obese + extract. model increases body weight, caloric intake, fat lipid profile. In tissue, P decreased SOD (superoxide dismutase) activity liver, positive modulation observed relation glucose, amino acids, lactate, hepatoprotective action, anti-inflammatory activity, decrease interleukin 1β (IL-1β) results showed improvement functional inflammatory aspects, but not effective alleviating general changes related obesity, such weight gain, fat, triglycerides, inflammation highlighting complexity responses different organs during heptaphyllum.

Язык: Английский

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