NAPTUNE: nucleic acids and protein biomarkers testing via ultra-sensitive nucleases escalation

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 4, 2025

Язык: Английский

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Nanoconfined Silver Nanoclusters Combined with X-Shaped DNA Recognizer-Triggered Cascade Amplification for Electrochemiluminescence Detection of APE1

Analytical Chemistry, Год журнала: 2024, Номер 96(28), С. 11383 - 11389

Опубликована: Июль 1, 2024

Apurinic/apyrimidinic endonuclease 1 (APE1), as a vital base excision repair enzyme, is essential for maintaining genomic integrity and stability, its abnormal expression closely associated with malignant tumors. Herein, we constructed an electrochemiluminescence (ECL) biosensor detecting APE1 activity by combining nanoconfined ECL silver nanoclusters (Ag NCs) X-shaped DNA recognizer-triggered cascade amplification. Specifically, the Ag NCs were prepared confined in glutaraldehyde-cross-linked chitosan hydrogel network using one-pot method, resulting strong response exceptional stability comparison discrete NCs. Furthermore, self-assembled recognizers designed detection, which not only improved reaction kinetics due to ordered arrangement of recognition sites but also achieved high sensitivity utilizing amplification strand displacement (SDA) DNAzyme catalysis. As expected, this sensitive detection range 1.0 × 10–3 U·μL–1 10–10 limit 2.21 10–11 U·μL–1, rendering it desirable approach biomarker detection.

Язык: Английский

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MiRNA and mRNA-Controlled Double-Cascaded Amplifying Circuit Nanosensor for Accurate Discrimination of Breast Cancers in Living Cells, Animals, and Organoids

Analytical Chemistry, Год журнала: 2024, Номер 96(10), С. 4154 - 4162

Опубликована: Март 1, 2024

Metastasis is the leading cause of death in patients with breast cancer. Detecting high-risk cancer, including micrometastasis, at an early stage vital for customizing right and efficient therapies. In this study, we propose enzyme-free isothermal cascade amplification-based DNA logic circuit situ biomineralization nanosensor, HDNAzyme@ZIF-8, simultaneous imaging multidimensional biomarkers live cells. Taking miR-21 Ki-67 mRNA as dual detection targets achieved sensitive operations molecular recognition through hybridization chain reaction DNAzyme. The HDNAzyme@ZIF-8 nanosensor has ability to accurately differentiate cancer cells their subtypes by comparing relative fluorescence intensities. Of note, our can also achieve visualization within organoids, faithfully recapitulating functional characteristics parental tumor. Overall, combination these techniques offers a universal strategy detecting cancers high sensitivity holds vast potential clinical diagnosis.

Язык: Английский

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Novel samarium-based metal-organic frameworks with antenna effect-induced electrochemiluminescence for acetamiprid assay

Sensors and Actuators B Chemical, Год журнала: 2025, Номер 430, С. 137343 - 137343

Опубликована: Янв. 29, 2025

Язык: Английский

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Label-free colorimetric detection platform based on catalytic hairpin self-assembly and G-quadruplex/hemin DNAzyme for comprehensive biomarker profiling

Talanta, Год журнала: 2024, Номер 272, С. 125835 - 125835

Опубликована: Фев. 25, 2024

Язык: Английский

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An ultrasensitive electrochemical biosensor based on logic transcription circuit-activated DNAzyme amplifier for flap endonuclease 1 activity detection

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 415, С. 135986 - 135986

Опубликована: Май 21, 2024

Язык: Английский

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Engineering Multi-activator-encoded DNA Nanonet to Accelerate CRISPR-Cas12a Activation for Rapid and Sensitive Electrochemiluminescence Bioassay

Talanta, Год журнала: 2025, Номер 288, С. 127724 - 127724

Опубликована: Фев. 11, 2025

Язык: Английский

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A dual-switch fluorescence biosensor with entropy-driven and DNA walker cascade amplification circuit for sensitive microRNA detection

Nanoscale, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

To achieve precise diagnosis of tumor cells, designing nucleic acid amplification circuits with intelligent multi-switch responsiveness for the specific and sensitive detection miRNA within cells is an important strategy. Here, we developed a dual-switch fluorescence biosensor that integrates two-step cascade signal circuit entropy-driven (EDC) DNA walkers onto gold nanoparticles highly quantitative target in cells. The dual switches trigger are APE1 enzyme, both which upregulated be specific, miRNA21 triggers upstream EDC, releasing strands serve as downstream circuit. Under cleavage-driven action walker walk along track on surface AuNPs, strong presenting good linearity concentration range 40 pM-100 nM. This presents specificity, anti-interference ability against multiple RNAs enzymes can effectively distinguish cancer from normal cell lysates. Overall, this provides recognition efficient strategy tumors, indicating its potential clinical applications disease diagnosis.

Язык: Английский

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Nicking Enzyme Assisted Amplification Combined with CRISPR-Cas12a System for One-Pot Sensitive Detection of APE1

The Analyst, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Apurinic/apyrimidinic endonuclease 1 (APE1) is a critical enzyme in the base excision repair (BER) pathway, essential for preserving cellular equilibrium. Variations APE1 activity within blood or tissues can provide significant insights clinical cancer screening and disease diagnosis. Consequently, detection of diagnostics. However, there currently deficiency rapid, straightforward, sensitive methods detection. To address this issue, we developed method that integrates Nicking Enzyme Assisted Amplification (NEAA) with CRISPR-Cas12a signal amplification, enabling one-pot activity. This utilizes NEAA to produce substantial quantity target DNA complementary crRNA, thereby triggering trans-cleavage Cas12a. The activated Cas12a then amplifies emits signals by cleaving reporter probe. Our strategy allows swift precise APE1, only 3 h, threshold × 10-6 U mL-1 linear range 5 0.1 mL-1. It has been effectively utilized biological samples.

Язык: Английский

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Isotope Dilution DNA Logic Circuits for Multiple Output and Absolute Quantification

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 6, 2025

DNA logic circuits have gained great success in the past, thanks to their distinct performance regarding scalability and correctness of computation. However, there are still two challenges often considered for circuit-based First, mainstream optical probes subject spectral overlapping interference complex multitask analysis outputs. Second, absolute quantification results traceable primary international system units mission impossible, especially interlaboratory comparisons quality assurances. Herein, we constructed encoded with lanthanide isotopes decoded by elemental mass spectrometry. The 155Gd-enriched isotope 145Nd-enriched were incorporated dilution-based microRNAs. proposed isotopic greatly enhance multiplexity computation accuracy, which poses a potential cancer biomarker-related diagnosis.

Язык: Английский

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